Biosynthesis of Ag nanoparticles using Salicornia bigelovii and its antibacterial activity
Background and aim: In recent years, the field of nanotechnology has become the most active area of research in modern material science. While many chemical- as well as physical methods are also used, green synthesis of nanoparticles is becoming the most evolved method of synthesis. In this study,...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Electronic Physician
2018-04-01
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| Series: | Electronic Physician |
| Subjects: | |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5984030/ |
| _version_ | 1818153647224127488 |
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| author | Mehrdad Khatami Fatemeh Golshan Noor Saeed Ahmadi Mohammadreza Aflatoonian |
| author_facet | Mehrdad Khatami Fatemeh Golshan Noor Saeed Ahmadi Mohammadreza Aflatoonian |
| author_sort | Mehrdad Khatami |
| collection | DOAJ |
| description | Background and aim: In recent years, the field of nanotechnology has become the most active area of research
in modern material science. While many chemical- as well as physical methods are also used, green synthesis of
nanoparticles is becoming the most evolved method of synthesis. In this study, we synthesized silver
nanoparticles from the seed extract of Salicornia bigelovii.
Methods: This experimental study was conducted from December 2017 to January 2018 in Kerman University of
Medical Sciences, Kerman, Iran. The effects of two concentrations (1m M and 4mM) on the synthesis of
nanoparticles were studied. Characterizations were done using different methods including ultraviolet (UV)
visible spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform
infrared spectroscopy (FTIR). Antibacterial activity of Ag nanoparticles against Staphylococcus aureus and
Escherichia coli was studied using microdilution method. The data were analyzed using Probit test in SPSS
(Version 20, USA).
Results: Formation of the AgNPs was confirmed by surface plasmon spectra using UV–Vis spectrophotometer
and absorbance peaks at 434 nm. The FTIR spectra showed the possible role of the functional group like carbonyl
groups in reduction of silver ions to silver nanoparticles. The XRD analysis showed that the synthesized silver
nanoparticles are of face-centered cubic structure. The TEM showed the formation of silver nanoparticles ranging
in diameter from 1 to 50 nm. The minimal inhibitory concentration and minimal bactericidal concentration of
AgNPs were determined for both S. aureus and E. coli 6.25 and 12.5 µg/mL, respectively.
Conclusion: An environmentally friendly approach is more affordable than chemical methods. Physicochemical
approaches can be harmful to the environment and to human health. Thus, the green synthesis methods are
simple, less expensive, and can cut consumption of energy; they can be used for synthesis of fixed nanoparticles
with preferred shape and size, without the use of toxic chemical agents. |
| first_indexed | 2024-12-11T14:13:56Z |
| format | Article |
| id | doaj.art-a77a234c62f048969f24b8f703cb9445 |
| institution | Directory Open Access Journal |
| issn | 2008-5842 2008-5842 |
| language | English |
| last_indexed | 2024-12-11T14:13:56Z |
| publishDate | 2018-04-01 |
| publisher | Electronic Physician |
| record_format | Article |
| series | Electronic Physician |
| spelling | doaj.art-a77a234c62f048969f24b8f703cb94452022-12-22T01:03:16ZengElectronic PhysicianElectronic Physician2008-58422008-58422018-04-011046733674010.19082/6733Biosynthesis of Ag nanoparticles using Salicornia bigelovii and its antibacterial activityMehrdad KhatamiFatemeh Golshan NoorSaeed AhmadiMohammadreza AflatoonianBackground and aim: In recent years, the field of nanotechnology has become the most active area of research in modern material science. While many chemical- as well as physical methods are also used, green synthesis of nanoparticles is becoming the most evolved method of synthesis. In this study, we synthesized silver nanoparticles from the seed extract of Salicornia bigelovii. Methods: This experimental study was conducted from December 2017 to January 2018 in Kerman University of Medical Sciences, Kerman, Iran. The effects of two concentrations (1m M and 4mM) on the synthesis of nanoparticles were studied. Characterizations were done using different methods including ultraviolet (UV) visible spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). Antibacterial activity of Ag nanoparticles against Staphylococcus aureus and Escherichia coli was studied using microdilution method. The data were analyzed using Probit test in SPSS (Version 20, USA). Results: Formation of the AgNPs was confirmed by surface plasmon spectra using UV–Vis spectrophotometer and absorbance peaks at 434 nm. The FTIR spectra showed the possible role of the functional group like carbonyl groups in reduction of silver ions to silver nanoparticles. The XRD analysis showed that the synthesized silver nanoparticles are of face-centered cubic structure. The TEM showed the formation of silver nanoparticles ranging in diameter from 1 to 50 nm. The minimal inhibitory concentration and minimal bactericidal concentration of AgNPs were determined for both S. aureus and E. coli 6.25 and 12.5 µg/mL, respectively. Conclusion: An environmentally friendly approach is more affordable than chemical methods. Physicochemical approaches can be harmful to the environment and to human health. Thus, the green synthesis methods are simple, less expensive, and can cut consumption of energy; they can be used for synthesis of fixed nanoparticles with preferred shape and size, without the use of toxic chemical agents.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5984030/X-Ray Absorption SpectroscopyBiosynthesisSilver |
| spellingShingle | Mehrdad Khatami Fatemeh Golshan Noor Saeed Ahmadi Mohammadreza Aflatoonian Biosynthesis of Ag nanoparticles using Salicornia bigelovii and its antibacterial activity Electronic Physician X-Ray Absorption Spectroscopy Biosynthesis Silver |
| title | Biosynthesis of Ag nanoparticles using Salicornia bigelovii and its antibacterial activity |
| title_full | Biosynthesis of Ag nanoparticles using Salicornia bigelovii and its antibacterial activity |
| title_fullStr | Biosynthesis of Ag nanoparticles using Salicornia bigelovii and its antibacterial activity |
| title_full_unstemmed | Biosynthesis of Ag nanoparticles using Salicornia bigelovii and its antibacterial activity |
| title_short | Biosynthesis of Ag nanoparticles using Salicornia bigelovii and its antibacterial activity |
| title_sort | biosynthesis of ag nanoparticles using salicornia bigelovii and its antibacterial activity |
| topic | X-Ray Absorption Spectroscopy Biosynthesis Silver |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5984030/ |
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