Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cells
Apico-basal polarity is the defining characteristic of epithelial cells. In Drosophila, apical membrane identity is established and regulated through interactions between the highly conserved Par complex (Bazooka/Par3, atypical protein kinase C and Par6), and the Crumbs complex (Crumbs, Stardust and...
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2015-03-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/4/4/528 |
| _version_ | 1819071071649792000 |
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| author | Jaffer Shahab Manu D. Tiwari Mona Honemann-Capito Michael P. Krahn Andreas Wodarz |
| author_facet | Jaffer Shahab Manu D. Tiwari Mona Honemann-Capito Michael P. Krahn Andreas Wodarz |
| author_sort | Jaffer Shahab |
| collection | DOAJ |
| description | Apico-basal polarity is the defining characteristic of epithelial cells. In Drosophila, apical membrane identity is established and regulated through interactions between the highly conserved Par complex (Bazooka/Par3, atypical protein kinase C and Par6), and the Crumbs complex (Crumbs, Stardust and PATJ). It has been proposed that Bazooka operates at the top of a genetic hierarchy in the establishment and maintenance of apico-basal polarity. However, there is still ambiguity over the correct sequence of events and cross-talk with other pathways during this process. In this study, we reassess this issue by comparing the phenotypes of the commonly used baz4 and baz815-8 alleles with those of the so far uncharacterized bazXR11 and bazEH747 null alleles in different Drosophila epithelia. While all these baz alleles display identical phenotypes during embryonic epithelial development, we observe strong discrepancies in the severity and penetrance of polarity defects in the follicular epithelium: polarity is mostly normal in bazEH747 and bazXR11 while baz4 and baz815-8 show loss of polarity, severe multilayering and loss of epithelial integrity throughout the clones. Further analysis reveals that the chromosomes carrying the baz4 and baz815-8 alleles may contain additional mutations that enhance the true baz loss-of-function phenotype in the follicular epithelium. This study clearly shows that Baz is dispensable for the regulation of polarity in the follicular epithelium, and that the requirement for key regulators of cell polarity is highly dependent on developmental context and cell type. |
| first_indexed | 2024-12-21T17:16:00Z |
| format | Article |
| id | doaj.art-a77ab8e632774578a68e6428826586b4 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-21T17:16:00Z |
| publishDate | 2015-03-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-a77ab8e632774578a68e6428826586b42022-12-21T18:56:18ZengThe Company of BiologistsBiology Open2046-63902015-03-014452854110.1242/bio.201410934201410934Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cellsJaffer Shahab0Manu D. Tiwari1Mona Honemann-Capito2Michael P. Krahn3Andreas Wodarz4 Stammzellbiologie, Institut für Anatomie und Zellbiologie, Georg-August Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany Stammzellbiologie, Institut für Anatomie und Zellbiologie, Georg-August Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany Stammzellbiologie, Institut für Anatomie und Zellbiologie, Georg-August Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany Stammzellbiologie, Institut für Anatomie und Zellbiologie, Georg-August Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany Stammzellbiologie, Institut für Anatomie und Zellbiologie, Georg-August Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany Apico-basal polarity is the defining characteristic of epithelial cells. In Drosophila, apical membrane identity is established and regulated through interactions between the highly conserved Par complex (Bazooka/Par3, atypical protein kinase C and Par6), and the Crumbs complex (Crumbs, Stardust and PATJ). It has been proposed that Bazooka operates at the top of a genetic hierarchy in the establishment and maintenance of apico-basal polarity. However, there is still ambiguity over the correct sequence of events and cross-talk with other pathways during this process. In this study, we reassess this issue by comparing the phenotypes of the commonly used baz4 and baz815-8 alleles with those of the so far uncharacterized bazXR11 and bazEH747 null alleles in different Drosophila epithelia. While all these baz alleles display identical phenotypes during embryonic epithelial development, we observe strong discrepancies in the severity and penetrance of polarity defects in the follicular epithelium: polarity is mostly normal in bazEH747 and bazXR11 while baz4 and baz815-8 show loss of polarity, severe multilayering and loss of epithelial integrity throughout the clones. Further analysis reveals that the chromosomes carrying the baz4 and baz815-8 alleles may contain additional mutations that enhance the true baz loss-of-function phenotype in the follicular epithelium. This study clearly shows that Baz is dispensable for the regulation of polarity in the follicular epithelium, and that the requirement for key regulators of cell polarity is highly dependent on developmental context and cell type.http://bio.biologists.org/content/4/4/528PolarityPar complexEpithelia |
| spellingShingle | Jaffer Shahab Manu D. Tiwari Mona Honemann-Capito Michael P. Krahn Andreas Wodarz Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cells Biology Open Polarity Par complex Epithelia |
| title | Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cells |
| title_full | Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cells |
| title_fullStr | Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cells |
| title_full_unstemmed | Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cells |
| title_short | Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cells |
| title_sort | bazooka par3 is dispensable for polarity in drosophila follicular epithelial cells |
| topic | Polarity Par complex Epithelia |
| url | http://bio.biologists.org/content/4/4/528 |
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