Changes in circulating microRNA and arterial stiffness following high‐intensity interval and moderate intensity continuous exercise
Abstract High‐intensity interval (HII) exercise elicits distinct vascular responses compared to a matched dose of moderate intensity continuous (MOD) exercise. However, the acute effects of HII compared to MOD exercise on arterial stiffness are incompletely understood. Circulating microRNAs (ci‐miRs...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-05-01
|
| Series: | Physiological Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.14814/phy2.14431 |
| _version_ | 1818529065015967744 |
|---|---|
| author | Ryan M. Sapp Catalina A. Chesney Lauren E. Eagan William S. Evans Evelyn M. Zietowski Steven J. Prior James M. Hagberg Sushant M. Ranadive |
| author_facet | Ryan M. Sapp Catalina A. Chesney Lauren E. Eagan William S. Evans Evelyn M. Zietowski Steven J. Prior James M. Hagberg Sushant M. Ranadive |
| author_sort | Ryan M. Sapp |
| collection | DOAJ |
| description | Abstract High‐intensity interval (HII) exercise elicits distinct vascular responses compared to a matched dose of moderate intensity continuous (MOD) exercise. However, the acute effects of HII compared to MOD exercise on arterial stiffness are incompletely understood. Circulating microRNAs (ci‐miRs) may contribute to the vascular effects of exercise. We sought to determine exercise intensity‐dependent changes in ci‐miR potentially underlying changes in arterial stiffness. Ten young, healthy men underwent well‐matched, 30‐min HII and MOD exercise bouts. RT‐qPCR was used to determine the levels of seven vascular‐related ci‐miRs in serum obtained immediately before and after exercise. Arterial stiffness measures including carotid to femoral pulse wave velocity (cf‐PWV), carotid arterial compliance and β‐stiffness, and augmentation index (AIx and AIx75) were taken before, 10min after and 60min after exercise. Ci‐miR‐21‐5p, 126‐3p, 126‐5p, 150‐5p, 155‐5p, and 181b‐5p increased after HII exercise (p < .05), while ci‐miR‐150‐5p and 221‐3p increased after MOD exercise (p = .03 and 0.056). One hour after HII exercise, cf‐PWV trended toward being lower compared to baseline (p = .056) and was significantly lower compared to 60min after MOD exercise (p = .04). Carotid arterial compliance was increased 60min after HII exercise (p = .049) and was greater than 60min after MOD exercise (p = .02). AIx75 increased 10 min after both HII and MOD exercise (p < .05). There were significant correlations between some of the exercise‐induced changes in individual ci‐miRs and changes in cf‐PWV and AIx/AIx75. These results support the hypotheses that arterial stiffness and ci‐miRs are altered in an exercise intensity‐dependent manner, and ci‐miRs may contribute to changes in arterial stiffness. |
| first_indexed | 2024-12-11T06:58:15Z |
| format | Article |
| id | doaj.art-a77b9e54a9374a77829324c16741bf28 |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-12-11T06:58:15Z |
| publishDate | 2020-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-a77b9e54a9374a77829324c16741bf282022-12-22T01:16:42ZengWileyPhysiological Reports2051-817X2020-05-0189n/an/a10.14814/phy2.14431Changes in circulating microRNA and arterial stiffness following high‐intensity interval and moderate intensity continuous exerciseRyan M. Sapp0Catalina A. Chesney1Lauren E. Eagan2William S. Evans3Evelyn M. Zietowski4Steven J. Prior5James M. Hagberg6Sushant M. Ranadive7Department of Kinesiology School of Public Health University of Maryland College Park MD USADepartment of Kinesiology School of Public Health University of Maryland College Park MD USADepartment of Kinesiology School of Public Health University of Maryland College Park MD USADepartment of Kinesiology School of Public Health University of Maryland College Park MD USADepartment of Kinesiology School of Public Health University of Maryland College Park MD USADepartment of Kinesiology School of Public Health University of Maryland College Park MD USADepartment of Kinesiology School of Public Health University of Maryland College Park MD USADepartment of Kinesiology School of Public Health University of Maryland College Park MD USAAbstract High‐intensity interval (HII) exercise elicits distinct vascular responses compared to a matched dose of moderate intensity continuous (MOD) exercise. However, the acute effects of HII compared to MOD exercise on arterial stiffness are incompletely understood. Circulating microRNAs (ci‐miRs) may contribute to the vascular effects of exercise. We sought to determine exercise intensity‐dependent changes in ci‐miR potentially underlying changes in arterial stiffness. Ten young, healthy men underwent well‐matched, 30‐min HII and MOD exercise bouts. RT‐qPCR was used to determine the levels of seven vascular‐related ci‐miRs in serum obtained immediately before and after exercise. Arterial stiffness measures including carotid to femoral pulse wave velocity (cf‐PWV), carotid arterial compliance and β‐stiffness, and augmentation index (AIx and AIx75) were taken before, 10min after and 60min after exercise. Ci‐miR‐21‐5p, 126‐3p, 126‐5p, 150‐5p, 155‐5p, and 181b‐5p increased after HII exercise (p < .05), while ci‐miR‐150‐5p and 221‐3p increased after MOD exercise (p = .03 and 0.056). One hour after HII exercise, cf‐PWV trended toward being lower compared to baseline (p = .056) and was significantly lower compared to 60min after MOD exercise (p = .04). Carotid arterial compliance was increased 60min after HII exercise (p = .049) and was greater than 60min after MOD exercise (p = .02). AIx75 increased 10 min after both HII and MOD exercise (p < .05). There were significant correlations between some of the exercise‐induced changes in individual ci‐miRs and changes in cf‐PWV and AIx/AIx75. These results support the hypotheses that arterial stiffness and ci‐miRs are altered in an exercise intensity‐dependent manner, and ci‐miRs may contribute to changes in arterial stiffness.https://doi.org/10.14814/phy2.14431Arterial stiffnessAugmentation indexHigh‐intensity interval exerciseMicroRNAPulse wave velocity |
| spellingShingle | Ryan M. Sapp Catalina A. Chesney Lauren E. Eagan William S. Evans Evelyn M. Zietowski Steven J. Prior James M. Hagberg Sushant M. Ranadive Changes in circulating microRNA and arterial stiffness following high‐intensity interval and moderate intensity continuous exercise Physiological Reports Arterial stiffness Augmentation index High‐intensity interval exercise MicroRNA Pulse wave velocity |
| title | Changes in circulating microRNA and arterial stiffness following high‐intensity interval and moderate intensity continuous exercise |
| title_full | Changes in circulating microRNA and arterial stiffness following high‐intensity interval and moderate intensity continuous exercise |
| title_fullStr | Changes in circulating microRNA and arterial stiffness following high‐intensity interval and moderate intensity continuous exercise |
| title_full_unstemmed | Changes in circulating microRNA and arterial stiffness following high‐intensity interval and moderate intensity continuous exercise |
| title_short | Changes in circulating microRNA and arterial stiffness following high‐intensity interval and moderate intensity continuous exercise |
| title_sort | changes in circulating microrna and arterial stiffness following high intensity interval and moderate intensity continuous exercise |
| topic | Arterial stiffness Augmentation index High‐intensity interval exercise MicroRNA Pulse wave velocity |
| url | https://doi.org/10.14814/phy2.14431 |
| work_keys_str_mv | AT ryanmsapp changesincirculatingmicrornaandarterialstiffnessfollowinghighintensityintervalandmoderateintensitycontinuousexercise AT catalinaachesney changesincirculatingmicrornaandarterialstiffnessfollowinghighintensityintervalandmoderateintensitycontinuousexercise AT laureneeagan changesincirculatingmicrornaandarterialstiffnessfollowinghighintensityintervalandmoderateintensitycontinuousexercise AT williamsevans changesincirculatingmicrornaandarterialstiffnessfollowinghighintensityintervalandmoderateintensitycontinuousexercise AT evelynmzietowski changesincirculatingmicrornaandarterialstiffnessfollowinghighintensityintervalandmoderateintensitycontinuousexercise AT stevenjprior changesincirculatingmicrornaandarterialstiffnessfollowinghighintensityintervalandmoderateintensitycontinuousexercise AT jamesmhagberg changesincirculatingmicrornaandarterialstiffnessfollowinghighintensityintervalandmoderateintensitycontinuousexercise AT sushantmranadive changesincirculatingmicrornaandarterialstiffnessfollowinghighintensityintervalandmoderateintensitycontinuousexercise |