KIOM-79 protects AGE-induced retinal pericyte apoptosis via inhibition of NF-kappaB activation in vitro and in vivo.
KIOM-79 is an herbal mixture of parched Puerariae radix, gingered Magnoliae cortex, Glycyrrhizae radix and Euphorbiae radix. In the present study, we determined the efficacy and possible mechanism of KIOM-79 on the advanced glycation end product (AGE)-modified bovine serum albumin (BSA)-induced apop...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3423361?pdf=render |
| _version_ | 1819113871478095872 |
|---|---|
| author | Junghyun Kim Chan-Sik Kim Eunjin Sohn Yun Mi Lee Kyuhyung Jo Jin Sook Kim |
| author_facet | Junghyun Kim Chan-Sik Kim Eunjin Sohn Yun Mi Lee Kyuhyung Jo Jin Sook Kim |
| author_sort | Junghyun Kim |
| collection | DOAJ |
| description | KIOM-79 is an herbal mixture of parched Puerariae radix, gingered Magnoliae cortex, Glycyrrhizae radix and Euphorbiae radix. In the present study, we determined the efficacy and possible mechanism of KIOM-79 on the advanced glycation end product (AGE)-modified bovine serum albumin (BSA)-induced apoptosis of cultured bovine retinal pericytes and rat retinal pericytes in Zucker diabetic fatty (ZDF) rats. Seven-week-old male ZDF rats were treated with KIOM-79 (50 mg/kg body weight) once a day orally for 13 weeks. KIOM-79 significantly inhibited pericyte apoptosis which were induced by the AGE-BSA treatment. The KIOM-79 treatment markedly suppressed the activation of nuclear factor-kappaB (NF-κB) through the inhibition of inhibitory κB kinase complex. In addition, the oral administration of KIOM-79 inhibited the changes in retinal vasculature (vascular hyperpermeability, acellular capillary). KIOM-79 strongly inhibited pericyte apoptosis, NF-κB activation and the expression of pro-apoptotic Bax and tumor necrosis factor-α. Our results suggest that KIOM-79 may exert inhibitory effects on AGE-induced pericyte apoptosis by blocking NF-κB activation, thereby ameliorating retinal microvascular dysfunction. |
| first_indexed | 2024-12-22T04:36:17Z |
| format | Article |
| id | doaj.art-a785496efa9b44f99474590ac5563273 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T04:36:17Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a785496efa9b44f99474590ac55632732022-12-21T18:38:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4359110.1371/journal.pone.0043591KIOM-79 protects AGE-induced retinal pericyte apoptosis via inhibition of NF-kappaB activation in vitro and in vivo.Junghyun KimChan-Sik KimEunjin SohnYun Mi LeeKyuhyung JoJin Sook KimKIOM-79 is an herbal mixture of parched Puerariae radix, gingered Magnoliae cortex, Glycyrrhizae radix and Euphorbiae radix. In the present study, we determined the efficacy and possible mechanism of KIOM-79 on the advanced glycation end product (AGE)-modified bovine serum albumin (BSA)-induced apoptosis of cultured bovine retinal pericytes and rat retinal pericytes in Zucker diabetic fatty (ZDF) rats. Seven-week-old male ZDF rats were treated with KIOM-79 (50 mg/kg body weight) once a day orally for 13 weeks. KIOM-79 significantly inhibited pericyte apoptosis which were induced by the AGE-BSA treatment. The KIOM-79 treatment markedly suppressed the activation of nuclear factor-kappaB (NF-κB) through the inhibition of inhibitory κB kinase complex. In addition, the oral administration of KIOM-79 inhibited the changes in retinal vasculature (vascular hyperpermeability, acellular capillary). KIOM-79 strongly inhibited pericyte apoptosis, NF-κB activation and the expression of pro-apoptotic Bax and tumor necrosis factor-α. Our results suggest that KIOM-79 may exert inhibitory effects on AGE-induced pericyte apoptosis by blocking NF-κB activation, thereby ameliorating retinal microvascular dysfunction.http://europepmc.org/articles/PMC3423361?pdf=render |
| spellingShingle | Junghyun Kim Chan-Sik Kim Eunjin Sohn Yun Mi Lee Kyuhyung Jo Jin Sook Kim KIOM-79 protects AGE-induced retinal pericyte apoptosis via inhibition of NF-kappaB activation in vitro and in vivo. PLoS ONE |
| title | KIOM-79 protects AGE-induced retinal pericyte apoptosis via inhibition of NF-kappaB activation in vitro and in vivo. |
| title_full | KIOM-79 protects AGE-induced retinal pericyte apoptosis via inhibition of NF-kappaB activation in vitro and in vivo. |
| title_fullStr | KIOM-79 protects AGE-induced retinal pericyte apoptosis via inhibition of NF-kappaB activation in vitro and in vivo. |
| title_full_unstemmed | KIOM-79 protects AGE-induced retinal pericyte apoptosis via inhibition of NF-kappaB activation in vitro and in vivo. |
| title_short | KIOM-79 protects AGE-induced retinal pericyte apoptosis via inhibition of NF-kappaB activation in vitro and in vivo. |
| title_sort | kiom 79 protects age induced retinal pericyte apoptosis via inhibition of nf kappab activation in vitro and in vivo |
| url | http://europepmc.org/articles/PMC3423361?pdf=render |
| work_keys_str_mv | AT junghyunkim kiom79protectsageinducedretinalpericyteapoptosisviainhibitionofnfkappabactivationinvitroandinvivo AT chansikkim kiom79protectsageinducedretinalpericyteapoptosisviainhibitionofnfkappabactivationinvitroandinvivo AT eunjinsohn kiom79protectsageinducedretinalpericyteapoptosisviainhibitionofnfkappabactivationinvitroandinvivo AT yunmilee kiom79protectsageinducedretinalpericyteapoptosisviainhibitionofnfkappabactivationinvitroandinvivo AT kyuhyungjo kiom79protectsageinducedretinalpericyteapoptosisviainhibitionofnfkappabactivationinvitroandinvivo AT jinsookkim kiom79protectsageinducedretinalpericyteapoptosisviainhibitionofnfkappabactivationinvitroandinvivo |