Effects of myeloperoxidase on inflammatory responses with hypoxia in Citrobacter rodentium‐infectious mice
Abstract Purpose Myeloperoxidase (MPO) has been identified as a mediator in various inflammatory diseases. Bacterial infection of the intestine and hypoxia can both lead to inflammatory responses, but the role of MPO in these phenomena remains unclear. Methods By building the MPO‐/‐ mice, we evaluat...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-02-01
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| Series: | Immunity, Inflammation and Disease |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/iid3.1157 |
| _version_ | 1827339230190239744 |
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| author | Xiang Gao Yu Zhang Qinfang Zhu Ying Han Ruhan Jia Wei Zhang |
| author_facet | Xiang Gao Yu Zhang Qinfang Zhu Ying Han Ruhan Jia Wei Zhang |
| author_sort | Xiang Gao |
| collection | DOAJ |
| description | Abstract Purpose Myeloperoxidase (MPO) has been identified as a mediator in various inflammatory diseases. Bacterial infection of the intestine and hypoxia can both lead to inflammatory responses, but the role of MPO in these phenomena remains unclear. Methods By building the MPO‐/‐ mice, we evaluated relevant inflammatory factors and tissue damage in mice with intestinal Citrobacter rodentium infection and hypoxia. The body weight and excreted microorganisms were monitored. Intestinal tissues were collected 7 days after bacterial infection under hypoxia to undergo haematoxylin‐eosin staining and assess the degree of pathological damage. ELISA assays were performed to quantify the serum levels of TNF‐α, IFN‐γ, IL‐6, and IL‐1β inflammatory cytokines. PCR, WB, and IF assays were conducted to determine the expression of chemokines MCP1, MIP2, and KC in the colon and spleen. Results The C. rodentium infection and hypoxia caused weight loss, intestinal colitis, and splenic inflammatory cells active proliferation in wild‐type mice. MPO deficiency alleviated this phenomenon. MPO‐/‐ mice also displayed a significant decline in bacteria clearing ability. The level of TNF‐α in the serum and spleen was both lower in MPO‐/‐ hypoxia C. rodentium‐infected mice than that in wild‐type mice. The chemokines expression levels of MIP2, KC, and MCP1 in the spleen and colon of each bacterial infected group were significantly increased (p < .05), while in hypoxia, the factors in the spleen and colon were decreased (p < .05). MPO deficiency was found to lower the levels of these chemokines compared with wild‐type mice. Conclusion MPO plays an important role of the inflammatory responses in infectious enteritis and hypoxia in mice, and the loss of MPO may greatly reduce the body's inflammatory responses to fight diseases. |
| first_indexed | 2024-03-07T19:47:35Z |
| format | Article |
| id | doaj.art-a7885c031cc94f739f78ee6e5ea59a8f |
| institution | Directory Open Access Journal |
| issn | 2050-4527 |
| language | English |
| last_indexed | 2024-03-07T19:47:35Z |
| publishDate | 2024-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Immunity, Inflammation and Disease |
| spelling | doaj.art-a7885c031cc94f739f78ee6e5ea59a8f2024-02-28T18:18:31ZengWileyImmunity, Inflammation and Disease2050-45272024-02-01122n/an/a10.1002/iid3.1157Effects of myeloperoxidase on inflammatory responses with hypoxia in Citrobacter rodentium‐infectious miceXiang Gao0Yu Zhang1Qinfang Zhu2Ying Han3Ruhan Jia4Wei Zhang5Department of Basic Medical Sciences, Medical College Qinghai University Xining Qinghai ChinaDepartment of Basic Medical Sciences, Medical College Qinghai University Xining Qinghai ChinaResearch Centre for High Altitude Medicine, Research Centre for High Altitude Medicine Qinghai University Xining Qinghai ChinaDepartment of Basic Medical Sciences, Medical College Qinghai University Xining Qinghai ChinaDepartment of Basic Medical Sciences, Medical College Qinghai University Xining Qinghai ChinaDepartment of Basic Medical Sciences, Medical College Qinghai University Xining Qinghai ChinaAbstract Purpose Myeloperoxidase (MPO) has been identified as a mediator in various inflammatory diseases. Bacterial infection of the intestine and hypoxia can both lead to inflammatory responses, but the role of MPO in these phenomena remains unclear. Methods By building the MPO‐/‐ mice, we evaluated relevant inflammatory factors and tissue damage in mice with intestinal Citrobacter rodentium infection and hypoxia. The body weight and excreted microorganisms were monitored. Intestinal tissues were collected 7 days after bacterial infection under hypoxia to undergo haematoxylin‐eosin staining and assess the degree of pathological damage. ELISA assays were performed to quantify the serum levels of TNF‐α, IFN‐γ, IL‐6, and IL‐1β inflammatory cytokines. PCR, WB, and IF assays were conducted to determine the expression of chemokines MCP1, MIP2, and KC in the colon and spleen. Results The C. rodentium infection and hypoxia caused weight loss, intestinal colitis, and splenic inflammatory cells active proliferation in wild‐type mice. MPO deficiency alleviated this phenomenon. MPO‐/‐ mice also displayed a significant decline in bacteria clearing ability. The level of TNF‐α in the serum and spleen was both lower in MPO‐/‐ hypoxia C. rodentium‐infected mice than that in wild‐type mice. The chemokines expression levels of MIP2, KC, and MCP1 in the spleen and colon of each bacterial infected group were significantly increased (p < .05), while in hypoxia, the factors in the spleen and colon were decreased (p < .05). MPO deficiency was found to lower the levels of these chemokines compared with wild‐type mice. Conclusion MPO plays an important role of the inflammatory responses in infectious enteritis and hypoxia in mice, and the loss of MPO may greatly reduce the body's inflammatory responses to fight diseases.https://doi.org/10.1002/iid3.1157colitishypoxiainflammatory responsesMPO |
| spellingShingle | Xiang Gao Yu Zhang Qinfang Zhu Ying Han Ruhan Jia Wei Zhang Effects of myeloperoxidase on inflammatory responses with hypoxia in Citrobacter rodentium‐infectious mice Immunity, Inflammation and Disease colitis hypoxia inflammatory responses MPO |
| title | Effects of myeloperoxidase on inflammatory responses with hypoxia in Citrobacter rodentium‐infectious mice |
| title_full | Effects of myeloperoxidase on inflammatory responses with hypoxia in Citrobacter rodentium‐infectious mice |
| title_fullStr | Effects of myeloperoxidase on inflammatory responses with hypoxia in Citrobacter rodentium‐infectious mice |
| title_full_unstemmed | Effects of myeloperoxidase on inflammatory responses with hypoxia in Citrobacter rodentium‐infectious mice |
| title_short | Effects of myeloperoxidase on inflammatory responses with hypoxia in Citrobacter rodentium‐infectious mice |
| title_sort | effects of myeloperoxidase on inflammatory responses with hypoxia in citrobacter rodentium infectious mice |
| topic | colitis hypoxia inflammatory responses MPO |
| url | https://doi.org/10.1002/iid3.1157 |
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