CircRNA GFRA1 promotes hepatocellular carcinoma progression by modulating the miR-498/NAP1L3 axis
Abstract Circular RNAs (circRNAs) play essential roles in tumorigenesis and tumor progression. CircRNA GFRA1 (circGFRA1) was dysregulated in many cancer samples and acted as an independent marker for prediction of survivals in various cancer patients. However, the functions and molecular mechanisms...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-79321-y |
| _version_ | 1818716218753810432 |
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| author | Shuai Lv Yingxia Li Hanbing Ning Meihui Zhang Qiaoyu Jia Xijuan Wang |
| author_facet | Shuai Lv Yingxia Li Hanbing Ning Meihui Zhang Qiaoyu Jia Xijuan Wang |
| author_sort | Shuai Lv |
| collection | DOAJ |
| description | Abstract Circular RNAs (circRNAs) play essential roles in tumorigenesis and tumor progression. CircRNA GFRA1 (circGFRA1) was dysregulated in many cancer samples and acted as an independent marker for prediction of survivals in various cancer patients. However, the functions and molecular mechanisms of circGFRA1 in hepatocellular carcinoma (HCC) remain unclear. We collected 62 HCC tissues and normal adjacent tissues to evaluate the expression of circGFRA1 and the relationship between circGFRA1 expression and HCC patients’ survival. We carried out a list of characterization experiments to investigate the roles and underling mechanisms of circGFRA1 and miR-498 in HCC progressions. CircGFRA1 was greatly increased in HCC tissues and cells, and the over-expression of circGFRA1 was intimately related with the advanced clinical stage and poor survival of HCC patients. The expression of circGFRA1 was negatively correlated with the expression of miR-498, but a positive correlation was found between circGFRA1 and NAP1L3 expression in HCC tissues. Silencing circGFRA1 inhibited the growth and invasion of hepatocellular carcinoma. Moreover, miR-498 over-expression or NAP1L3 inhibition could abrogate the oncogene role of circGFRA1 in HCC in vivo. Our findings indicated that circGFRA1 contributed to HCC progression by modulating the miR-498/NAP1L3 axis in HCC. |
| first_indexed | 2024-12-17T19:15:46Z |
| format | Article |
| id | doaj.art-a78df13550b34a77af16de39faa15a30 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-17T19:15:46Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a78df13550b34a77af16de39faa15a302022-12-21T21:35:45ZengNature PortfolioScientific Reports2045-23222021-01-0111111010.1038/s41598-020-79321-yCircRNA GFRA1 promotes hepatocellular carcinoma progression by modulating the miR-498/NAP1L3 axisShuai Lv0Yingxia Li1Hanbing Ning2Meihui Zhang3Qiaoyu Jia4Xijuan Wang5Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou UniversityDepartment of Gastroenterology, The First Affiliated Hospital of Zhengzhou UniversityDepartment of Gastroenterology, The First Affiliated Hospital of Zhengzhou UniversityDepartment of Pediatrics, Henan Provincial People’s Hospital; Zhengzhou University People’s Hospital; Henan University People’s HospitalDepartment of Gastroenterology, The First Affiliated Hospital of Zhengzhou UniversityDepartment of Pediatrics, Henan Provincial People’s Hospital; Zhengzhou University People’s Hospital; Henan University People’s HospitalAbstract Circular RNAs (circRNAs) play essential roles in tumorigenesis and tumor progression. CircRNA GFRA1 (circGFRA1) was dysregulated in many cancer samples and acted as an independent marker for prediction of survivals in various cancer patients. However, the functions and molecular mechanisms of circGFRA1 in hepatocellular carcinoma (HCC) remain unclear. We collected 62 HCC tissues and normal adjacent tissues to evaluate the expression of circGFRA1 and the relationship between circGFRA1 expression and HCC patients’ survival. We carried out a list of characterization experiments to investigate the roles and underling mechanisms of circGFRA1 and miR-498 in HCC progressions. CircGFRA1 was greatly increased in HCC tissues and cells, and the over-expression of circGFRA1 was intimately related with the advanced clinical stage and poor survival of HCC patients. The expression of circGFRA1 was negatively correlated with the expression of miR-498, but a positive correlation was found between circGFRA1 and NAP1L3 expression in HCC tissues. Silencing circGFRA1 inhibited the growth and invasion of hepatocellular carcinoma. Moreover, miR-498 over-expression or NAP1L3 inhibition could abrogate the oncogene role of circGFRA1 in HCC in vivo. Our findings indicated that circGFRA1 contributed to HCC progression by modulating the miR-498/NAP1L3 axis in HCC.https://doi.org/10.1038/s41598-020-79321-y |
| spellingShingle | Shuai Lv Yingxia Li Hanbing Ning Meihui Zhang Qiaoyu Jia Xijuan Wang CircRNA GFRA1 promotes hepatocellular carcinoma progression by modulating the miR-498/NAP1L3 axis Scientific Reports |
| title | CircRNA GFRA1 promotes hepatocellular carcinoma progression by modulating the miR-498/NAP1L3 axis |
| title_full | CircRNA GFRA1 promotes hepatocellular carcinoma progression by modulating the miR-498/NAP1L3 axis |
| title_fullStr | CircRNA GFRA1 promotes hepatocellular carcinoma progression by modulating the miR-498/NAP1L3 axis |
| title_full_unstemmed | CircRNA GFRA1 promotes hepatocellular carcinoma progression by modulating the miR-498/NAP1L3 axis |
| title_short | CircRNA GFRA1 promotes hepatocellular carcinoma progression by modulating the miR-498/NAP1L3 axis |
| title_sort | circrna gfra1 promotes hepatocellular carcinoma progression by modulating the mir 498 nap1l3 axis |
| url | https://doi.org/10.1038/s41598-020-79321-y |
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