Recombinant Photolyase-Thymine Alleviated UVB-Induced Photodamage in Mice by Repairing CPD Photoproducts and Ameliorating Oxidative Stress
Cyclobutane pyrimidine dimers (CPDs) are the main mutagenic DNA photoproducts caused by ultraviolet B (UVB) radiation and represent the major cause of photoaging and skin carcinogenesis. CPD photolyase can efficiently and rapidly repair CPD products. Therefore, they are candidates for the prevention...
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MDPI AG
2022-11-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/12/2312 |
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| author | Zhaoyang Wang Ziyi Li Yaling Lei Yuan Liu Yuqing Feng Derong Chen Siying Ma Ziyan Xiao Meirong Hu Jingxian Deng Yuxin Wang Qihao Zhang Yadong Huang Yan Yang |
| author_facet | Zhaoyang Wang Ziyi Li Yaling Lei Yuan Liu Yuqing Feng Derong Chen Siying Ma Ziyan Xiao Meirong Hu Jingxian Deng Yuxin Wang Qihao Zhang Yadong Huang Yan Yang |
| author_sort | Zhaoyang Wang |
| collection | DOAJ |
| description | Cyclobutane pyrimidine dimers (CPDs) are the main mutagenic DNA photoproducts caused by ultraviolet B (UVB) radiation and represent the major cause of photoaging and skin carcinogenesis. CPD photolyase can efficiently and rapidly repair CPD products. Therefore, they are candidates for the prevention of photodamage. However, these photolyases are not present in placental mammals. In this study, we produced a recombinant photolyase-thymine (rPHO) from <i>Thermus thermophilus</i> (<i>T. thermophilus</i>). The rPHO displayed CPD photorepair activity. It prevented UVB-induced DNA damage by repairing CPD photoproducts to pyrimidine monomers. Furthermore, it inhibited UVB-induced ROS production, lipid peroxidation, inflammatory responses, and apoptosis. UVB-induced wrinkle formation, epidermal hyperplasia, and collagen degradation in mice skin was significantly inhibited when the photolyase was applied topically to the skin. These results demonstrated that rPHO has promising protective effects against UVB-induced photodamage and may contribute to the development of anti-UVB skin photodamage drugs and cosmetic products. |
| first_indexed | 2024-03-09T17:24:15Z |
| format | Article |
| id | doaj.art-a79080d77d1c4215b2e02ee9308cd790 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T17:24:15Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-a79080d77d1c4215b2e02ee9308cd7902023-11-24T12:55:53ZengMDPI AGAntioxidants2076-39212022-11-011112231210.3390/antiox11122312Recombinant Photolyase-Thymine Alleviated UVB-Induced Photodamage in Mice by Repairing CPD Photoproducts and Ameliorating Oxidative StressZhaoyang Wang0Ziyi Li1Yaling Lei2Yuan Liu3Yuqing Feng4Derong Chen5Siying Ma6Ziyan Xiao7Meirong Hu8Jingxian Deng9Yuxin Wang10Qihao Zhang11Yadong Huang12Yan Yang13Department of Cell Biology, Jinan University, Guangzhou 510632, ChinaTYRAN Cosmetics Innovation Research Institute, Jinan University, Guangzhou 511447, ChinaDepartment of Cell Biology, Jinan University, Guangzhou 510632, ChinaDepartment of Cell Biology, Jinan University, Guangzhou 510632, ChinaDepartment of Pharmacology, Jinan University, Guangzhou 510632, ChinaDepartment of Cell Biology, Jinan University, Guangzhou 510632, ChinaDepartment of Cell Biology, Jinan University, Guangzhou 510632, ChinaDepartment of Cell Biology, Jinan University, Guangzhou 510632, ChinaDepartment of Cell Biology, Jinan University, Guangzhou 510632, ChinaDepartment of Pharmacology, Jinan University, Guangzhou 510632, ChinaDepartment of Cell Biology, Jinan University, Guangzhou 510632, ChinaDepartment of Cell Biology, Jinan University, Guangzhou 510632, ChinaDepartment of Cell Biology, Jinan University, Guangzhou 510632, ChinaDepartment of Cell Biology, Jinan University, Guangzhou 510632, ChinaCyclobutane pyrimidine dimers (CPDs) are the main mutagenic DNA photoproducts caused by ultraviolet B (UVB) radiation and represent the major cause of photoaging and skin carcinogenesis. CPD photolyase can efficiently and rapidly repair CPD products. Therefore, they are candidates for the prevention of photodamage. However, these photolyases are not present in placental mammals. In this study, we produced a recombinant photolyase-thymine (rPHO) from <i>Thermus thermophilus</i> (<i>T. thermophilus</i>). The rPHO displayed CPD photorepair activity. It prevented UVB-induced DNA damage by repairing CPD photoproducts to pyrimidine monomers. Furthermore, it inhibited UVB-induced ROS production, lipid peroxidation, inflammatory responses, and apoptosis. UVB-induced wrinkle formation, epidermal hyperplasia, and collagen degradation in mice skin was significantly inhibited when the photolyase was applied topically to the skin. These results demonstrated that rPHO has promising protective effects against UVB-induced photodamage and may contribute to the development of anti-UVB skin photodamage drugs and cosmetic products.https://www.mdpi.com/2076-3921/11/12/2312recombinant photolyasephotorepairDNA damageoxidative stress |
| spellingShingle | Zhaoyang Wang Ziyi Li Yaling Lei Yuan Liu Yuqing Feng Derong Chen Siying Ma Ziyan Xiao Meirong Hu Jingxian Deng Yuxin Wang Qihao Zhang Yadong Huang Yan Yang Recombinant Photolyase-Thymine Alleviated UVB-Induced Photodamage in Mice by Repairing CPD Photoproducts and Ameliorating Oxidative Stress Antioxidants recombinant photolyase photorepair DNA damage oxidative stress |
| title | Recombinant Photolyase-Thymine Alleviated UVB-Induced Photodamage in Mice by Repairing CPD Photoproducts and Ameliorating Oxidative Stress |
| title_full | Recombinant Photolyase-Thymine Alleviated UVB-Induced Photodamage in Mice by Repairing CPD Photoproducts and Ameliorating Oxidative Stress |
| title_fullStr | Recombinant Photolyase-Thymine Alleviated UVB-Induced Photodamage in Mice by Repairing CPD Photoproducts and Ameliorating Oxidative Stress |
| title_full_unstemmed | Recombinant Photolyase-Thymine Alleviated UVB-Induced Photodamage in Mice by Repairing CPD Photoproducts and Ameliorating Oxidative Stress |
| title_short | Recombinant Photolyase-Thymine Alleviated UVB-Induced Photodamage in Mice by Repairing CPD Photoproducts and Ameliorating Oxidative Stress |
| title_sort | recombinant photolyase thymine alleviated uvb induced photodamage in mice by repairing cpd photoproducts and ameliorating oxidative stress |
| topic | recombinant photolyase photorepair DNA damage oxidative stress |
| url | https://www.mdpi.com/2076-3921/11/12/2312 |
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