Serum biomarkers and anti-flavivirus antibodies at presentation as indicators of severe dengue.
<h4>Background</h4>Dengue is the most common vector-borne viral disease worldwide. Most cases are mild, but some evolve into severe dengue (SD), with high lethality. Therefore, it is important to identify biomarkers of severe disease to improve outcomes and judiciously utilize resources....
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-02-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0010750 |
| _version_ | 1797866794071883776 |
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| author | Cynthia Bernal Sara Ping Alejandra Rojas Oliver Caballero Victoria Stittleburg Yvalena de Guillén Patricia Langjahr Benjamin A Pinsky Marta Von-Horoch Patricia Luraschi Sandra Cabral María Cecilia Sánchez Aurelia Torres Fátima Cardozo Jesse J Waggoner |
| author_facet | Cynthia Bernal Sara Ping Alejandra Rojas Oliver Caballero Victoria Stittleburg Yvalena de Guillén Patricia Langjahr Benjamin A Pinsky Marta Von-Horoch Patricia Luraschi Sandra Cabral María Cecilia Sánchez Aurelia Torres Fátima Cardozo Jesse J Waggoner |
| author_sort | Cynthia Bernal |
| collection | DOAJ |
| description | <h4>Background</h4>Dengue is the most common vector-borne viral disease worldwide. Most cases are mild, but some evolve into severe dengue (SD), with high lethality. Therefore, it is important to identify biomarkers of severe disease to improve outcomes and judiciously utilize resources.<h4>Methods/principal findings</h4>One hundred forty-five confirmed dengue cases (median age, 42; range <1-91 years), enrolled from February 2018 to March 2020, were selected from an ongoing study of suspected arboviral infections in metropolitan Asunción, Paraguay. Cases included dengue virus types 1, 2, and 4, and severity was categorized according to the 2009 World Health Organization guidelines. Testing for anti-dengue virus IgM and IgG and serum biomarkers (lipopolysaccharide binding protein and chymase) was performed on acute-phase sera in plate-based ELISAs; in addition, a multiplex ELISA platform was used to measure anti-dengue virus and anti-Zika virus IgM and IgG. Complete blood counts and chemistries were performed at the discretion of the care team. Age, gender, and pre-existing comorbidities were associated with SD vs. dengue with/without warning signs in logistic regression with odds ratios (ORs) of 1.07 (per year; 95% confidence interval, 1.03, 1.11), 0.20 (female; 0.05,0.77), and 2.09 (presence; 1.26, 3.48) respectively. In binary logistic regression, for every unit increase in anti-DENV IgG in the multiplex platform, odds of SD increased by 2.54 (1.19-5.42). Platelet count, lymphocyte percent, and elevated chymase were associated with SD in a combined logistic regression model with ORs of 0.99 (1,000/μL; 0.98,0.999), 0.92 (%; 0.86,0.98), and 1.17 (mg/mL; 1.03,1.33) respectively.<h4>Conclusions</h4>Multiple, readily available factors were associated with SD in this population. These findings will aid in the early detection of potentially severe dengue cases and inform the development of new prognostics for use in acute-phase and serial samples from dengue cases. |
| first_indexed | 2024-04-09T23:31:01Z |
| format | Article |
| id | doaj.art-a795043f02d24e2fb09a2e50eca8ab91 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-04-09T23:31:01Z |
| publishDate | 2023-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-a795043f02d24e2fb09a2e50eca8ab912023-03-21T05:32:53ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352023-02-01172e001075010.1371/journal.pntd.0010750Serum biomarkers and anti-flavivirus antibodies at presentation as indicators of severe dengue.Cynthia BernalSara PingAlejandra RojasOliver CaballeroVictoria StittleburgYvalena de GuillénPatricia LangjahrBenjamin A PinskyMarta Von-HorochPatricia LuraschiSandra CabralMaría Cecilia SánchezAurelia TorresFátima CardozoJesse J Waggoner<h4>Background</h4>Dengue is the most common vector-borne viral disease worldwide. Most cases are mild, but some evolve into severe dengue (SD), with high lethality. Therefore, it is important to identify biomarkers of severe disease to improve outcomes and judiciously utilize resources.<h4>Methods/principal findings</h4>One hundred forty-five confirmed dengue cases (median age, 42; range <1-91 years), enrolled from February 2018 to March 2020, were selected from an ongoing study of suspected arboviral infections in metropolitan Asunción, Paraguay. Cases included dengue virus types 1, 2, and 4, and severity was categorized according to the 2009 World Health Organization guidelines. Testing for anti-dengue virus IgM and IgG and serum biomarkers (lipopolysaccharide binding protein and chymase) was performed on acute-phase sera in plate-based ELISAs; in addition, a multiplex ELISA platform was used to measure anti-dengue virus and anti-Zika virus IgM and IgG. Complete blood counts and chemistries were performed at the discretion of the care team. Age, gender, and pre-existing comorbidities were associated with SD vs. dengue with/without warning signs in logistic regression with odds ratios (ORs) of 1.07 (per year; 95% confidence interval, 1.03, 1.11), 0.20 (female; 0.05,0.77), and 2.09 (presence; 1.26, 3.48) respectively. In binary logistic regression, for every unit increase in anti-DENV IgG in the multiplex platform, odds of SD increased by 2.54 (1.19-5.42). Platelet count, lymphocyte percent, and elevated chymase were associated with SD in a combined logistic regression model with ORs of 0.99 (1,000/μL; 0.98,0.999), 0.92 (%; 0.86,0.98), and 1.17 (mg/mL; 1.03,1.33) respectively.<h4>Conclusions</h4>Multiple, readily available factors were associated with SD in this population. These findings will aid in the early detection of potentially severe dengue cases and inform the development of new prognostics for use in acute-phase and serial samples from dengue cases.https://doi.org/10.1371/journal.pntd.0010750 |
| spellingShingle | Cynthia Bernal Sara Ping Alejandra Rojas Oliver Caballero Victoria Stittleburg Yvalena de Guillén Patricia Langjahr Benjamin A Pinsky Marta Von-Horoch Patricia Luraschi Sandra Cabral María Cecilia Sánchez Aurelia Torres Fátima Cardozo Jesse J Waggoner Serum biomarkers and anti-flavivirus antibodies at presentation as indicators of severe dengue. PLoS Neglected Tropical Diseases |
| title | Serum biomarkers and anti-flavivirus antibodies at presentation as indicators of severe dengue. |
| title_full | Serum biomarkers and anti-flavivirus antibodies at presentation as indicators of severe dengue. |
| title_fullStr | Serum biomarkers and anti-flavivirus antibodies at presentation as indicators of severe dengue. |
| title_full_unstemmed | Serum biomarkers and anti-flavivirus antibodies at presentation as indicators of severe dengue. |
| title_short | Serum biomarkers and anti-flavivirus antibodies at presentation as indicators of severe dengue. |
| title_sort | serum biomarkers and anti flavivirus antibodies at presentation as indicators of severe dengue |
| url | https://doi.org/10.1371/journal.pntd.0010750 |
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