Cadmium resistance of endophytic bacteria and rizosféricas bacteria isolated from Oriza sativa in Colombia
The present study had as objective to evaluate in vitro the resistance of endophytic bacteria and rizospheric bacteria to different concentrations of Cadmium.This bacteria were isolated fron different tissues of commercial rice varieties and from bacteria isolated from the rhizosphere in rice planta...
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Format: | Article |
Language: | English |
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Universidad de Sucre
2017-12-01
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Series: | Revista Colombiana de Ciencia Animal Recia |
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Online Access: | http://revistas.unisucre.edu.co/index.php/recia/article/view/610 |
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author | Nataly Ayubb T Armando Cerra G Leonardo Chamorro A Alexander Pérez C |
author_facet | Nataly Ayubb T Armando Cerra G Leonardo Chamorro A Alexander Pérez C |
author_sort | Nataly Ayubb T |
collection | DOAJ |
description | The present study had as objective to evaluate in vitro the resistance of endophytic bacteria and rizospheric bacteria to different concentrations of Cadmium.This bacteria were isolated fron different tissues of commercial rice varieties and from bacteria isolated from the rhizosphere in rice plantations of the Nechí (Antioquía) and Achí (Bolivar). Plant growth promotion was evaluated in vitro by nitrogen fixation, phosphate solubilization and siderophores production of endophytic bacteria. Of each tissue isolated from rice plants was carried out isolation in culture medium for endophytic bacteria, and the soil samples were serially diluted in peptone water. Each sample was determined the population density by counting in CFU / g of tissue and morphotypes were separated by shape, color, size and appearance in culture media. Significant differences were observed for density population of bacteria with respect to tissue, with higher values in root (4x1011 g/root), followed of the stem (3x1010g/etem), leaf (5x109 g/ leaf), flag leaf (3x109 g/ flag leaf) and with less density in panicle (4x108 g/panicle). The results of the identification with kit API were confirmed the presence of endophytic bacteria Burkholderia cepaceae and rizospheric bacteria Pseudomona fluorescens With the ability to tolerate different concentrations of Cd, fix nitrogen, solubilize phosphates and produce siderophores. |
first_indexed | 2024-12-11T06:57:30Z |
format | Article |
id | doaj.art-a795ce0f55ad4d60ab9cc3d8835478d5 |
institution | Directory Open Access Journal |
issn | 2027-4297 |
language | English |
last_indexed | 2024-12-11T06:57:30Z |
publishDate | 2017-12-01 |
publisher | Universidad de Sucre |
record_format | Article |
series | Revista Colombiana de Ciencia Animal Recia |
spelling | doaj.art-a795ce0f55ad4d60ab9cc3d8835478d52022-12-22T01:16:44ZengUniversidad de SucreRevista Colombiana de Ciencia Animal Recia2027-42972017-12-019228129310.24188/recia.v9.n2.2017.610538Cadmium resistance of endophytic bacteria and rizosféricas bacteria isolated from Oriza sativa in ColombiaNataly Ayubb T0Armando Cerra G1Leonardo Chamorro A2Alexander Pérez C3Universidad de Sucre, Facultad de Educación y Ciencias, Ciencias Ambientales.Universidad de Sucre, Facultad de Educación y Ciencias, Ciencias Ambientales.Universidad de Sucre, Facultad de Ciencias Agropecuarias, Grupo Bioprospección Agropecuaria.Universidad de Sucre, Facultad de Ciencias Agropecuarias, Grupo Bioprospección AgropecuariaThe present study had as objective to evaluate in vitro the resistance of endophytic bacteria and rizospheric bacteria to different concentrations of Cadmium.This bacteria were isolated fron different tissues of commercial rice varieties and from bacteria isolated from the rhizosphere in rice plantations of the Nechí (Antioquía) and Achí (Bolivar). Plant growth promotion was evaluated in vitro by nitrogen fixation, phosphate solubilization and siderophores production of endophytic bacteria. Of each tissue isolated from rice plants was carried out isolation in culture medium for endophytic bacteria, and the soil samples were serially diluted in peptone water. Each sample was determined the population density by counting in CFU / g of tissue and morphotypes were separated by shape, color, size and appearance in culture media. Significant differences were observed for density population of bacteria with respect to tissue, with higher values in root (4x1011 g/root), followed of the stem (3x1010g/etem), leaf (5x109 g/ leaf), flag leaf (3x109 g/ flag leaf) and with less density in panicle (4x108 g/panicle). The results of the identification with kit API were confirmed the presence of endophytic bacteria Burkholderia cepaceae and rizospheric bacteria Pseudomona fluorescens With the ability to tolerate different concentrations of Cd, fix nitrogen, solubilize phosphates and produce siderophores.http://revistas.unisucre.edu.co/index.php/recia/article/view/610Bacteria endófitarizósferatoleranciaCadmio |
spellingShingle | Nataly Ayubb T Armando Cerra G Leonardo Chamorro A Alexander Pérez C Cadmium resistance of endophytic bacteria and rizosféricas bacteria isolated from Oriza sativa in Colombia Revista Colombiana de Ciencia Animal Recia Bacteria endófita rizósfera tolerancia Cadmio |
title | Cadmium resistance of endophytic bacteria and rizosféricas bacteria isolated from Oriza sativa in Colombia |
title_full | Cadmium resistance of endophytic bacteria and rizosféricas bacteria isolated from Oriza sativa in Colombia |
title_fullStr | Cadmium resistance of endophytic bacteria and rizosféricas bacteria isolated from Oriza sativa in Colombia |
title_full_unstemmed | Cadmium resistance of endophytic bacteria and rizosféricas bacteria isolated from Oriza sativa in Colombia |
title_short | Cadmium resistance of endophytic bacteria and rizosféricas bacteria isolated from Oriza sativa in Colombia |
title_sort | cadmium resistance of endophytic bacteria and rizosfericas bacteria isolated from oriza sativa in colombia |
topic | Bacteria endófita rizósfera tolerancia Cadmio |
url | http://revistas.unisucre.edu.co/index.php/recia/article/view/610 |
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