To Evaluate the Different Rapid Screening Tests for Diagnosis of Leptospirosis
Introduction: Leptospirosis is an acute febrile disease, in tropical and sub-tropical regions of world. It has been underreported in India, due to presence of non-specific symptoms and unavailability of appropriate laboratory diagnostic facilities in most part of the country. The diagnosis of lep...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2015-02-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/5587/11188_CE(RA1)_F(T)_PF1(NJAK)_PFA(AK)_PF2(PAG).pdf |
| _version_ | 1828849035548557312 |
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| author | Tanvi Panwala Sangeeta Rajdev Summaiya Mulla |
| author_facet | Tanvi Panwala Sangeeta Rajdev Summaiya Mulla |
| author_sort | Tanvi Panwala |
| collection | DOAJ |
| description | Introduction: Leptospirosis is an acute febrile disease, in
tropical and sub-tropical regions of world. It has been underreported in India, due to presence of non-specific symptoms
and unavailability of appropriate laboratory diagnostic facilities in
most part of the country. The diagnosis of leptospirosis is usually
based on demonstration of antibodies by different serological
tests.
Aim: The present study aims to evaluate and compare commercially available rapid test.
Design and Settings: Case control study.
Materials and Methods: Three screening tests (Leptocheck WB,
Latex agglutination test and SD leptospira) were compared by
using 100 serum samples randomly obtained from clinical cases
of Leptospirosis admitted in new civil hospital, Surat, Gujarat.
All the patients with acute Leptospirosis were included in this
4-months pilot study from July 2011 to October 2011. All the
results were compared with IgM ELISA and MAT for confirmation
of diagnosis.
Results: Leptocheck WB, Latex agglutination test and SD
leptospira had sensitivities of 84.8%, 84.8% and 72.7% &
specificities of 37.3%, 71.2% and 71.2% respectively as compared to MAT. Leptocheck WB, Latex agglutination test and
SD leptospira had sensitivities of 90.7%, 89.7% and 53.7% &
specificities of 93.4%, 90.9% and 60% respectively as compared
to IgM ELISA.
Conclusion: Latex agglutination test kit and Leptocheck WB
were found to be highly sensitive and specific. Neither of these
tests require specialized equipment, and could be performed in
peripheral laboratories with relatively little expertise. |
| first_indexed | 2024-12-12T22:42:39Z |
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| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-12T22:42:39Z |
| publishDate | 2015-02-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-a79ca0b95d4b4c6ba6ebe8d823ad22a62022-12-22T00:09:18ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2015-02-0192DC21DC2410.7860/JCDR/2015/11188.5587To Evaluate the Different Rapid Screening Tests for Diagnosis of LeptospirosisTanvi Panwala0Sangeeta Rajdev1Summaiya Mulla2Assistant Professor, Department of Microbiology, Government Medical College, Surat, India.Assistant Professor, Department of Microbiology, Government Medical College, Surat, India.Professor and Head, Department of Microbiology, Government Medical College, Surat, India.Introduction: Leptospirosis is an acute febrile disease, in tropical and sub-tropical regions of world. It has been underreported in India, due to presence of non-specific symptoms and unavailability of appropriate laboratory diagnostic facilities in most part of the country. The diagnosis of leptospirosis is usually based on demonstration of antibodies by different serological tests. Aim: The present study aims to evaluate and compare commercially available rapid test. Design and Settings: Case control study. Materials and Methods: Three screening tests (Leptocheck WB, Latex agglutination test and SD leptospira) were compared by using 100 serum samples randomly obtained from clinical cases of Leptospirosis admitted in new civil hospital, Surat, Gujarat. All the patients with acute Leptospirosis were included in this 4-months pilot study from July 2011 to October 2011. All the results were compared with IgM ELISA and MAT for confirmation of diagnosis. Results: Leptocheck WB, Latex agglutination test and SD leptospira had sensitivities of 84.8%, 84.8% and 72.7% & specificities of 37.3%, 71.2% and 71.2% respectively as compared to MAT. Leptocheck WB, Latex agglutination test and SD leptospira had sensitivities of 90.7%, 89.7% and 53.7% & specificities of 93.4%, 90.9% and 60% respectively as compared to IgM ELISA. Conclusion: Latex agglutination test kit and Leptocheck WB were found to be highly sensitive and specific. Neither of these tests require specialized equipment, and could be performed in peripheral laboratories with relatively little expertise.https://jcdr.net/articles/PDF/5587/11188_CE(RA1)_F(T)_PF1(NJAK)_PFA(AK)_PF2(PAG).pdfigm elisaleptospirosisleptorapideleptocheckmatsd leptospira igm/igg |
| spellingShingle | Tanvi Panwala Sangeeta Rajdev Summaiya Mulla To Evaluate the Different Rapid Screening Tests for Diagnosis of Leptospirosis Journal of Clinical and Diagnostic Research igm elisa leptospirosis leptorapide leptocheck mat sd leptospira igm/igg |
| title | To Evaluate the Different Rapid Screening Tests for Diagnosis of Leptospirosis |
| title_full | To Evaluate the Different Rapid Screening Tests for Diagnosis of Leptospirosis |
| title_fullStr | To Evaluate the Different Rapid Screening Tests for Diagnosis of Leptospirosis |
| title_full_unstemmed | To Evaluate the Different Rapid Screening Tests for Diagnosis of Leptospirosis |
| title_short | To Evaluate the Different Rapid Screening Tests for Diagnosis of Leptospirosis |
| title_sort | to evaluate the different rapid screening tests for diagnosis of leptospirosis |
| topic | igm elisa leptospirosis leptorapide leptocheck mat sd leptospira igm/igg |
| url | https://jcdr.net/articles/PDF/5587/11188_CE(RA1)_F(T)_PF1(NJAK)_PFA(AK)_PF2(PAG).pdf |
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