Changes in oral microflora following 0.3% cetylpyridinium chloride‐containing mouth spray intervention in adult volunteers after professional oral care: Randomized clinical study
Abstract Objectives This study explored the changes in bacterial flora composition and total bacterial count in the saliva and tongue coating, along with the change in the tongue coating index (TCI) following an intervention with 0.3% cetylpyridinium chloride (CPC) mouth spray after professional ora...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-12-01
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| Series: | Clinical and Experimental Dental Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cre2.810 |
| _version_ | 1797386650470318080 |
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| author | Ai Fujimoto Kana Fujii Hirohisa Suido Hisae Fukuike Naoko Miyake Hidenori Suzuki Toru Eguchi Haruko Tobata |
| author_facet | Ai Fujimoto Kana Fujii Hirohisa Suido Hisae Fukuike Naoko Miyake Hidenori Suzuki Toru Eguchi Haruko Tobata |
| author_sort | Ai Fujimoto |
| collection | DOAJ |
| description | Abstract Objectives This study explored the changes in bacterial flora composition and total bacterial count in the saliva and tongue coating, along with the change in the tongue coating index (TCI) following an intervention with 0.3% cetylpyridinium chloride (CPC) mouth spray after professional oral care. Materials and Methods Fifty‐two adult volunteers aged 30–60 years were equally divided into CPC spray (n = 26) and control (n = 26) groups. All subjects underwent scaling and polishing. The CPC spray group was administered four puffs of CPC spray to the tongue dorsum four times a day for 3 weeks. The control group performed only routine daily oral care (brushing) and did not use any other spray. Bacteriological evaluation of saliva and tongue coating was performed using 16S ribosomal RNA gene sequencing and quantitative polymerase chain reaction. The tongue coating was evaluated to calculate the TCI. A per‐protocol analysis was conducted for 44 subjects (CPC spray group, n = 23; control group, n = 21). Results At 1 and 3 weeks after CPC spray use, the flora of the saliva and tongue coating changed; the genus Haemophilus was dominant in the CPC spray group, whereas the genus Saccharibacteria was dominant in the control group. The sampling time differed among individual participants, which may have affected the bacterial counts. There was no significant intragroup change in TCI in either group. Conclusions CPC spray affected the bacterial flora in the saliva and tongue coating, particularly with respect to an increase in the abundance of Haemophilus. However, CPC spray did not change the TCI. These results suggest that it may be optimal to combine CPC spray with a physical cleaning method such as using a tongue brush or scraper. Clinical Trial Registration: University Hospital Medical Information Network UMIN000041140. |
| first_indexed | 2024-03-08T22:12:35Z |
| format | Article |
| id | doaj.art-a79db48213d84398af1fa8bb7951267d |
| institution | Directory Open Access Journal |
| issn | 2057-4347 |
| language | English |
| last_indexed | 2024-03-08T22:12:35Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Experimental Dental Research |
| spelling | doaj.art-a79db48213d84398af1fa8bb7951267d2023-12-19T05:49:29ZengWileyClinical and Experimental Dental Research2057-43472023-12-01961034104310.1002/cre2.810Changes in oral microflora following 0.3% cetylpyridinium chloride‐containing mouth spray intervention in adult volunteers after professional oral care: Randomized clinical studyAi Fujimoto0Kana Fujii1Hirohisa Suido2Hisae Fukuike3Naoko Miyake4Hidenori Suzuki5Toru Eguchi6Haruko Tobata7Research and Development, Sunstar Inc. Osaka JapanResearch and Development, Sunstar Inc. Osaka JapanDepartment of Health and Nutrition, Faculty of Health Science Kyoto Koka Women's University Kyoto JapanOral Health Promotion, Affiliated with the Sunstar Foundation Osaka JapanSunstar Senri Dental Clinic, Affiliated with the Sunstar Foundation Osaka JapanSunstar Senri Dental Clinic, Affiliated with the Sunstar Foundation Osaka JapanResearch and Development, Sunstar Inc. Osaka JapanResearch and Development, Sunstar Inc. Osaka JapanAbstract Objectives This study explored the changes in bacterial flora composition and total bacterial count in the saliva and tongue coating, along with the change in the tongue coating index (TCI) following an intervention with 0.3% cetylpyridinium chloride (CPC) mouth spray after professional oral care. Materials and Methods Fifty‐two adult volunteers aged 30–60 years were equally divided into CPC spray (n = 26) and control (n = 26) groups. All subjects underwent scaling and polishing. The CPC spray group was administered four puffs of CPC spray to the tongue dorsum four times a day for 3 weeks. The control group performed only routine daily oral care (brushing) and did not use any other spray. Bacteriological evaluation of saliva and tongue coating was performed using 16S ribosomal RNA gene sequencing and quantitative polymerase chain reaction. The tongue coating was evaluated to calculate the TCI. A per‐protocol analysis was conducted for 44 subjects (CPC spray group, n = 23; control group, n = 21). Results At 1 and 3 weeks after CPC spray use, the flora of the saliva and tongue coating changed; the genus Haemophilus was dominant in the CPC spray group, whereas the genus Saccharibacteria was dominant in the control group. The sampling time differed among individual participants, which may have affected the bacterial counts. There was no significant intragroup change in TCI in either group. Conclusions CPC spray affected the bacterial flora in the saliva and tongue coating, particularly with respect to an increase in the abundance of Haemophilus. However, CPC spray did not change the TCI. These results suggest that it may be optimal to combine CPC spray with a physical cleaning method such as using a tongue brush or scraper. Clinical Trial Registration: University Hospital Medical Information Network UMIN000041140.https://doi.org/10.1002/cre2.81016S rRNACetylpyridinium ChlorideClinical Studies as TopicHuman Microbiome |
| spellingShingle | Ai Fujimoto Kana Fujii Hirohisa Suido Hisae Fukuike Naoko Miyake Hidenori Suzuki Toru Eguchi Haruko Tobata Changes in oral microflora following 0.3% cetylpyridinium chloride‐containing mouth spray intervention in adult volunteers after professional oral care: Randomized clinical study Clinical and Experimental Dental Research 16S rRNA Cetylpyridinium Chloride Clinical Studies as Topic Human Microbiome |
| title | Changes in oral microflora following 0.3% cetylpyridinium chloride‐containing mouth spray intervention in adult volunteers after professional oral care: Randomized clinical study |
| title_full | Changes in oral microflora following 0.3% cetylpyridinium chloride‐containing mouth spray intervention in adult volunteers after professional oral care: Randomized clinical study |
| title_fullStr | Changes in oral microflora following 0.3% cetylpyridinium chloride‐containing mouth spray intervention in adult volunteers after professional oral care: Randomized clinical study |
| title_full_unstemmed | Changes in oral microflora following 0.3% cetylpyridinium chloride‐containing mouth spray intervention in adult volunteers after professional oral care: Randomized clinical study |
| title_short | Changes in oral microflora following 0.3% cetylpyridinium chloride‐containing mouth spray intervention in adult volunteers after professional oral care: Randomized clinical study |
| title_sort | changes in oral microflora following 0 3 cetylpyridinium chloride containing mouth spray intervention in adult volunteers after professional oral care randomized clinical study |
| topic | 16S rRNA Cetylpyridinium Chloride Clinical Studies as Topic Human Microbiome |
| url | https://doi.org/10.1002/cre2.810 |
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