Study on Doxorubicin Loading on Differently Functionalized Iron Oxide Nanoparticles: Implications for Controlled Drug-Delivery Application
Nanoplatforms applied for the loading of anticancer drugs is a cutting-edge approach for drug delivery to tumors and reduction of toxic effects on healthy cells. In this study, we describe the synthesis and compare the sorption properties of four types of potential doxorubicin-carriers, in which iro...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/5/4480 |
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| author | Vladislav R. Khabibullin Margarita R. Chetyrkina Sergei I. Obydennyy Sergey V. Maksimov Gennady V. Stepanov Sergei N. Shtykov |
| author_facet | Vladislav R. Khabibullin Margarita R. Chetyrkina Sergei I. Obydennyy Sergey V. Maksimov Gennady V. Stepanov Sergei N. Shtykov |
| author_sort | Vladislav R. Khabibullin |
| collection | DOAJ |
| description | Nanoplatforms applied for the loading of anticancer drugs is a cutting-edge approach for drug delivery to tumors and reduction of toxic effects on healthy cells. In this study, we describe the synthesis and compare the sorption properties of four types of potential doxorubicin-carriers, in which iron oxide nanoparticles (IONs) are functionalized with cationic (polyethylenimine, PEI), anionic (polystyrenesulfonate, PSS), and nonionic (dextran) polymers, as well as with porous carbon. The IONs are thoroughly characterized by X-ray diffraction, IR spectroscopy, high resolution TEM (HRTEM), SEM, magnetic susceptibility, and the zeta-potential measurements in the pH range of 3–10. The degree of doxorubicin loading at pH 7.4, as well as the degree of desorption at pH 5.0, distinctive to cancerous tumor environment, are measured. Particles modified with PEI were shown to exhibit the highest loading capacity, while the greatest release at pH 5 (up to 30%) occurs from the surface of magnetite decorated with PSS. Such a slow release of the drug would imply a prolonged tumor-inhibiting action on the affected tissue or organ. Assessment of the toxicity (using Neuro2A cell line) for PEI- and PSS-modified IONs showed no negative effect. In conclusion, the preliminary evaluation of the effects of IONs coated with PSS and PEI on the rate of blood clotting was carried out. The results obtained can be taken into account when developing new drug delivery platforms. |
| first_indexed | 2024-03-11T07:23:47Z |
| format | Article |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T07:23:47Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a79e3c22eed54d2c84e486c83ac6c7ac2023-11-17T07:49:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01245448010.3390/ijms24054480Study on Doxorubicin Loading on Differently Functionalized Iron Oxide Nanoparticles: Implications for Controlled Drug-Delivery ApplicationVladislav R. Khabibullin0Margarita R. Chetyrkina1Sergei I. Obydennyy2Sergey V. Maksimov3Gennady V. Stepanov4Sergei N. Shtykov5Chemistry Department, Lomonosov Moscow State University, Lenin Hills, 119991 Moscow, RussiaSkolkovo Institute of Science and Technology, 143026 Moscow, RussiaDmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, 117198 Moscow, RussiaChemistry Department, Lomonosov Moscow State University, Lenin Hills, 119991 Moscow, RussiaState Scientific Center of the Russian Federation, Joint Stock Company “State Order of the Red Banner of Labor Research Institute of Chemistry and Technology of Organoelement Compounds”, 105118 Moscow, RussiaDepartment of Analytical Chemistry and Chemical Ecology, Institute of Chemistry, Saratov State University, 410012 Saratov, RussiaNanoplatforms applied for the loading of anticancer drugs is a cutting-edge approach for drug delivery to tumors and reduction of toxic effects on healthy cells. In this study, we describe the synthesis and compare the sorption properties of four types of potential doxorubicin-carriers, in which iron oxide nanoparticles (IONs) are functionalized with cationic (polyethylenimine, PEI), anionic (polystyrenesulfonate, PSS), and nonionic (dextran) polymers, as well as with porous carbon. The IONs are thoroughly characterized by X-ray diffraction, IR spectroscopy, high resolution TEM (HRTEM), SEM, magnetic susceptibility, and the zeta-potential measurements in the pH range of 3–10. The degree of doxorubicin loading at pH 7.4, as well as the degree of desorption at pH 5.0, distinctive to cancerous tumor environment, are measured. Particles modified with PEI were shown to exhibit the highest loading capacity, while the greatest release at pH 5 (up to 30%) occurs from the surface of magnetite decorated with PSS. Such a slow release of the drug would imply a prolonged tumor-inhibiting action on the affected tissue or organ. Assessment of the toxicity (using Neuro2A cell line) for PEI- and PSS-modified IONs showed no negative effect. In conclusion, the preliminary evaluation of the effects of IONs coated with PSS and PEI on the rate of blood clotting was carried out. The results obtained can be taken into account when developing new drug delivery platforms.https://www.mdpi.com/1422-0067/24/5/4480doxorubicinsorptioniron oxide nanoparticlessurface functionalizationmagnetic propertiescell viability |
| spellingShingle | Vladislav R. Khabibullin Margarita R. Chetyrkina Sergei I. Obydennyy Sergey V. Maksimov Gennady V. Stepanov Sergei N. Shtykov Study on Doxorubicin Loading on Differently Functionalized Iron Oxide Nanoparticles: Implications for Controlled Drug-Delivery Application International Journal of Molecular Sciences doxorubicin sorption iron oxide nanoparticles surface functionalization magnetic properties cell viability |
| title | Study on Doxorubicin Loading on Differently Functionalized Iron Oxide Nanoparticles: Implications for Controlled Drug-Delivery Application |
| title_full | Study on Doxorubicin Loading on Differently Functionalized Iron Oxide Nanoparticles: Implications for Controlled Drug-Delivery Application |
| title_fullStr | Study on Doxorubicin Loading on Differently Functionalized Iron Oxide Nanoparticles: Implications for Controlled Drug-Delivery Application |
| title_full_unstemmed | Study on Doxorubicin Loading on Differently Functionalized Iron Oxide Nanoparticles: Implications for Controlled Drug-Delivery Application |
| title_short | Study on Doxorubicin Loading on Differently Functionalized Iron Oxide Nanoparticles: Implications for Controlled Drug-Delivery Application |
| title_sort | study on doxorubicin loading on differently functionalized iron oxide nanoparticles implications for controlled drug delivery application |
| topic | doxorubicin sorption iron oxide nanoparticles surface functionalization magnetic properties cell viability |
| url | https://www.mdpi.com/1422-0067/24/5/4480 |
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