Rodents as intermediate hosts of cestode parasites of mammalian carnivores and birds of prey in Poland, with the first data on the life-cycle of Mesocestoides melesi
Abstract Background Rodents constitute an important part of the diet of many carnivore species. This predator-prey food chain is exploited by helminth parasites, such as cestodes, whose larval stages develop in rodents and then mature to the adult stage in predators. The main aim of our study was to...
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| Format: | Article |
| Language: | English |
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BMC
2020-02-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-020-3961-2 |
| _version_ | 1818577435443068928 |
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| author | Anna Bajer Mohammed Alsarraf Dorota Dwużnik Ewa J. Mierzejewska Marta Kołodziej-Sobocińska Jolanta Behnke-Borowczyk Łukasz Banasiak Maciej Grzybek Katarzyna Tołkacz Natalia Kartawik Łukasz Stańczak Patrycja Opalińska Małgorzata Krokowska-Paluszak Grzegorz Górecki Mustafa Alsarraf Jerzy M. Behnke |
| author_facet | Anna Bajer Mohammed Alsarraf Dorota Dwużnik Ewa J. Mierzejewska Marta Kołodziej-Sobocińska Jolanta Behnke-Borowczyk Łukasz Banasiak Maciej Grzybek Katarzyna Tołkacz Natalia Kartawik Łukasz Stańczak Patrycja Opalińska Małgorzata Krokowska-Paluszak Grzegorz Górecki Mustafa Alsarraf Jerzy M. Behnke |
| author_sort | Anna Bajer |
| collection | DOAJ |
| description | Abstract Background Rodents constitute an important part of the diet of many carnivore species. This predator-prey food chain is exploited by helminth parasites, such as cestodes, whose larval stages develop in rodents and then mature to the adult stage in predators. The main aim of our study was to use molecular techniques for identification of cestode species recovered from both intermediate and definitive hosts, with a particular focus on the genus Mesocestoides. Methods Larval cestodes were obtained during our long-term studies on rodent helminth communities in the Mazury Lake District in the north-east Poland in 2000–2018. Cestode larvae/cysts were collected from body cavities or internal organs (e.g. liver) during autopsies. Adult tapeworms were derived from nine red foxes, three Eurasian badgers and one Eurasian lynx. PCR amplification, sequencing and phylogenetic analyses were conducted employing three genetic markers: 18S rDNA, mitochondrial (mt) 12S rDNA and the mt cytochrome c oxydase subunit 1 (cox1) gene fragment. Results Altogether 19 Mesocestoides samples were analyzed, including 13 adult tapeworms from definitive hosts and six larval samples from 4 bank voles and 2 yellow-necked mice. Phylogenetic analyses revealed three well-supported trees of similar topology. In each case the Mesocestoides samples formed two separate clades. All isolates from foxes, the lynx isolate and two isolates from rodents grouped with Mesocestoides litteratus. Four isolates from rodents and all three isolates from Eurasian badgers were resolved in a separate clade, most similar to North American M. vogae (syn. M. corti). Examination of fixed, stained adult specimens from Eurasian badgers revealed consistency with the morphology of Mesocestoides melesi. Therefore, this clade is likely to represent M. melesi, a species first described in 1985 from the Eurasian badger Meles meles. Molecular analysis allowed also the identification of Taenia crassiceps, Hydatigera kamiyai and Cladotaenia globifera among larvae derived from rodents. Conclusions Molecular and phylogenetic analyses support the recognition of M. melesi as a valid species. Our data represent the first record of the larvae of this species in rodents. This is the first report on the occurrence of H. kamiyai in rodents from Poland. |
| first_indexed | 2024-12-16T06:29:52Z |
| format | Article |
| id | doaj.art-a7a0cbfd1fe44c1c90e42a29e87fa994 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-16T06:29:52Z |
| publishDate | 2020-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-a7a0cbfd1fe44c1c90e42a29e87fa9942022-12-21T22:40:55ZengBMCParasites & Vectors1756-33052020-02-0113111010.1186/s13071-020-3961-2Rodents as intermediate hosts of cestode parasites of mammalian carnivores and birds of prey in Poland, with the first data on the life-cycle of Mesocestoides melesiAnna Bajer0Mohammed Alsarraf1Dorota Dwużnik2Ewa J. Mierzejewska3Marta Kołodziej-Sobocińska4Jolanta Behnke-Borowczyk5Łukasz Banasiak6Maciej Grzybek7Katarzyna Tołkacz8Natalia Kartawik9Łukasz Stańczak10Patrycja Opalińska11Małgorzata Krokowska-Paluszak12Grzegorz Górecki13Mustafa Alsarraf14Jerzy M. Behnke15Department of Eco-Epidemiology of Parasitic Diseases, Faculty of Biology, University of WarsawDepartment of Eco-Epidemiology of Parasitic Diseases, Faculty of Biology, University of WarsawDepartment of Eco-Epidemiology of Parasitic Diseases, Faculty of Biology, University of WarsawDepartment of Eco-Epidemiology of Parasitic Diseases, Faculty of Biology, University of WarsawMammal Research Institute, Polish Academy of SciencesDepartment of Forest Phytopathology, Faculty of Forestry, Poznań University of Life SciencesDepartment of Molecular Phylogenetics and Evolution, Institute of Botany, Faculty of Biology, University of Warsaw, Biological and Chemical Research CentreDepartment of Tropical Parasitology, Institute of Maritime and Tropical Medicine, University of GdanskDepartment of Eco-Epidemiology of Parasitic Diseases, Faculty of Biology, University of WarsawDepartment of Forest Phytopathology, Faculty of Forestry, Poznań University of Life SciencesDepartment of Game Management and Forest Protection, Faculty of Forestry, Poznań University of Life SciencesDepartment of Game Management and Forest Protection, Faculty of Forestry, Poznań University of Life SciencesDepartment of Game Management and Forest Protection, Faculty of Forestry, Poznań University of Life SciencesDepartment of Game Management and Forest Protection, Faculty of Forestry, Poznań University of Life SciencesDepartment of Eco-Epidemiology of Parasitic Diseases, Faculty of Biology, University of WarsawSchool of Life Sciences, University of NottinghamAbstract Background Rodents constitute an important part of the diet of many carnivore species. This predator-prey food chain is exploited by helminth parasites, such as cestodes, whose larval stages develop in rodents and then mature to the adult stage in predators. The main aim of our study was to use molecular techniques for identification of cestode species recovered from both intermediate and definitive hosts, with a particular focus on the genus Mesocestoides. Methods Larval cestodes were obtained during our long-term studies on rodent helminth communities in the Mazury Lake District in the north-east Poland in 2000–2018. Cestode larvae/cysts were collected from body cavities or internal organs (e.g. liver) during autopsies. Adult tapeworms were derived from nine red foxes, three Eurasian badgers and one Eurasian lynx. PCR amplification, sequencing and phylogenetic analyses were conducted employing three genetic markers: 18S rDNA, mitochondrial (mt) 12S rDNA and the mt cytochrome c oxydase subunit 1 (cox1) gene fragment. Results Altogether 19 Mesocestoides samples were analyzed, including 13 adult tapeworms from definitive hosts and six larval samples from 4 bank voles and 2 yellow-necked mice. Phylogenetic analyses revealed three well-supported trees of similar topology. In each case the Mesocestoides samples formed two separate clades. All isolates from foxes, the lynx isolate and two isolates from rodents grouped with Mesocestoides litteratus. Four isolates from rodents and all three isolates from Eurasian badgers were resolved in a separate clade, most similar to North American M. vogae (syn. M. corti). Examination of fixed, stained adult specimens from Eurasian badgers revealed consistency with the morphology of Mesocestoides melesi. Therefore, this clade is likely to represent M. melesi, a species first described in 1985 from the Eurasian badger Meles meles. Molecular analysis allowed also the identification of Taenia crassiceps, Hydatigera kamiyai and Cladotaenia globifera among larvae derived from rodents. Conclusions Molecular and phylogenetic analyses support the recognition of M. melesi as a valid species. Our data represent the first record of the larvae of this species in rodents. This is the first report on the occurrence of H. kamiyai in rodents from Poland.http://link.springer.com/article/10.1186/s13071-020-3961-2MesocestoidesHydatigeraTaenia crassicepsRodentsFoxBadger |
| spellingShingle | Anna Bajer Mohammed Alsarraf Dorota Dwużnik Ewa J. Mierzejewska Marta Kołodziej-Sobocińska Jolanta Behnke-Borowczyk Łukasz Banasiak Maciej Grzybek Katarzyna Tołkacz Natalia Kartawik Łukasz Stańczak Patrycja Opalińska Małgorzata Krokowska-Paluszak Grzegorz Górecki Mustafa Alsarraf Jerzy M. Behnke Rodents as intermediate hosts of cestode parasites of mammalian carnivores and birds of prey in Poland, with the first data on the life-cycle of Mesocestoides melesi Parasites & Vectors Mesocestoides Hydatigera Taenia crassiceps Rodents Fox Badger |
| title | Rodents as intermediate hosts of cestode parasites of mammalian carnivores and birds of prey in Poland, with the first data on the life-cycle of Mesocestoides melesi |
| title_full | Rodents as intermediate hosts of cestode parasites of mammalian carnivores and birds of prey in Poland, with the first data on the life-cycle of Mesocestoides melesi |
| title_fullStr | Rodents as intermediate hosts of cestode parasites of mammalian carnivores and birds of prey in Poland, with the first data on the life-cycle of Mesocestoides melesi |
| title_full_unstemmed | Rodents as intermediate hosts of cestode parasites of mammalian carnivores and birds of prey in Poland, with the first data on the life-cycle of Mesocestoides melesi |
| title_short | Rodents as intermediate hosts of cestode parasites of mammalian carnivores and birds of prey in Poland, with the first data on the life-cycle of Mesocestoides melesi |
| title_sort | rodents as intermediate hosts of cestode parasites of mammalian carnivores and birds of prey in poland with the first data on the life cycle of mesocestoides melesi |
| topic | Mesocestoides Hydatigera Taenia crassiceps Rodents Fox Badger |
| url | http://link.springer.com/article/10.1186/s13071-020-3961-2 |
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