Combination Therapy with Losartan and α-Tocopherol in Acute Ureteral Obstruction-Induced Renal Excretory Dysfunction and Acidification Defect
Background: Previous study by the authors showed that -tocopherol prevents oxidative stress but would not improve depressed excretory variables in post-obstructed kidney (POK) after release of 24-h unilateral ureteral obstruction (UUO). This study is a supplementary investigation on the effects of...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2014-07-01
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| Series: | Iranian Journal of Medical Sciences |
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| Online Access: | http://ijms.sums.ac.ir/index.php/IJMS/article/view/950 |
| _version_ | 1818826765100908544 |
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| author | Izadpanah Gheitasi Seyed Mostafa Moosavi |
| author_facet | Izadpanah Gheitasi Seyed Mostafa Moosavi |
| author_sort | Izadpanah Gheitasi |
| collection | DOAJ |
| description | Background: Previous study by the authors showed that -tocopherol prevents oxidative stress but would not improve depressed excretory variables in post-obstructed kidney (POK) after release of 24-h unilateral ureteral obstruction (UUO). This study is a supplementary investigation on the effects of -tocopherol combined with an antagonist of angiotensin-II type-1 (AT1) receptor on renal dysfunction following release of acute UUO.
Methods: The left ureter was ligated in different groups of male Sprague-Dawley rats that received normal saline, losartan or losartan/-tocopherol (n=6 in each group). After releasing 24-h UUO, urine of each kidney was separately collected under paraffin during 1-3 h of post-release period and then both kidneys were removed for measuring malondialdehyde (MDA) and ferric reducing/antioxidant power (FRAP).
Results: Losartan-treatment decreased MDA and increased FRAP, creatinine-clearance and sodium-reabsorption in POK, while co-treatment with losartan and -tocopherol not only augmented improvement in these variables but also elevated potassium-excretion, free-water reabsorption and urine-osmolality. However, UUO-induced fall in urinary pCO2 and rise in pH and bicarbonate-excretion of POK were ameliorated equally with losartan and losartan/-tocopherol.
Conclusion: Activation of AT1-receptor contributes to the development of renal distal acidification defect induced by acute ureteral obstruction. The co-treatment with losartan and -tocopherol showed that their effects on preventing oxidative stress along with ameliorating glomerular filtration and tubular fluid-delivery in POK could lead to improvement in tubular transport of sodium and potassium as well as urine-concentrating ability at the early post-release period. |
| first_indexed | 2024-12-19T00:32:51Z |
| format | Article |
| id | doaj.art-a7a15bf5653c48b3b5b0769496735942 |
| institution | Directory Open Access Journal |
| issn | 0253-0716 1735-3688 |
| language | English |
| last_indexed | 2024-12-19T00:32:51Z |
| publishDate | 2014-07-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Medical Sciences |
| spelling | doaj.art-a7a15bf5653c48b3b5b07694967359422022-12-21T20:45:01ZengShiraz University of Medical SciencesIranian Journal of Medical Sciences0253-07161735-36882014-07-01394357366Combination Therapy with Losartan and α-Tocopherol in Acute Ureteral Obstruction-Induced Renal Excretory Dysfunction and Acidification DefectIzadpanah Gheitasi0Seyed Mostafa Moosavi1Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranBackground: Previous study by the authors showed that -tocopherol prevents oxidative stress but would not improve depressed excretory variables in post-obstructed kidney (POK) after release of 24-h unilateral ureteral obstruction (UUO). This study is a supplementary investigation on the effects of -tocopherol combined with an antagonist of angiotensin-II type-1 (AT1) receptor on renal dysfunction following release of acute UUO. Methods: The left ureter was ligated in different groups of male Sprague-Dawley rats that received normal saline, losartan or losartan/-tocopherol (n=6 in each group). After releasing 24-h UUO, urine of each kidney was separately collected under paraffin during 1-3 h of post-release period and then both kidneys were removed for measuring malondialdehyde (MDA) and ferric reducing/antioxidant power (FRAP). Results: Losartan-treatment decreased MDA and increased FRAP, creatinine-clearance and sodium-reabsorption in POK, while co-treatment with losartan and -tocopherol not only augmented improvement in these variables but also elevated potassium-excretion, free-water reabsorption and urine-osmolality. However, UUO-induced fall in urinary pCO2 and rise in pH and bicarbonate-excretion of POK were ameliorated equally with losartan and losartan/-tocopherol. Conclusion: Activation of AT1-receptor contributes to the development of renal distal acidification defect induced by acute ureteral obstruction. The co-treatment with losartan and -tocopherol showed that their effects on preventing oxidative stress along with ameliorating glomerular filtration and tubular fluid-delivery in POK could lead to improvement in tubular transport of sodium and potassium as well as urine-concentrating ability at the early post-release period.http://ijms.sums.ac.ir/index.php/IJMS/article/view/950Alfa-tocopherolLosartanRenal tubular acidosisUreteral obstruction |
| spellingShingle | Izadpanah Gheitasi Seyed Mostafa Moosavi Combination Therapy with Losartan and α-Tocopherol in Acute Ureteral Obstruction-Induced Renal Excretory Dysfunction and Acidification Defect Iranian Journal of Medical Sciences Alfa-tocopherol Losartan Renal tubular acidosis Ureteral obstruction |
| title | Combination Therapy with Losartan and α-Tocopherol in Acute Ureteral Obstruction-Induced Renal Excretory Dysfunction and Acidification Defect |
| title_full | Combination Therapy with Losartan and α-Tocopherol in Acute Ureteral Obstruction-Induced Renal Excretory Dysfunction and Acidification Defect |
| title_fullStr | Combination Therapy with Losartan and α-Tocopherol in Acute Ureteral Obstruction-Induced Renal Excretory Dysfunction and Acidification Defect |
| title_full_unstemmed | Combination Therapy with Losartan and α-Tocopherol in Acute Ureteral Obstruction-Induced Renal Excretory Dysfunction and Acidification Defect |
| title_short | Combination Therapy with Losartan and α-Tocopherol in Acute Ureteral Obstruction-Induced Renal Excretory Dysfunction and Acidification Defect |
| title_sort | combination therapy with losartan and α tocopherol in acute ureteral obstruction induced renal excretory dysfunction and acidification defect |
| topic | Alfa-tocopherol Losartan Renal tubular acidosis Ureteral obstruction |
| url | http://ijms.sums.ac.ir/index.php/IJMS/article/view/950 |
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