The Relation between Microalbuminuria and Helicobacter Pylori VacA Gene in Type 2 Diabetic Patients, Isfahan, Iran
Background: Diabetes mellitus is the most prevalent metabolic disorder worldwide with many subsequent medical complications. An important complication of diabetes is microalbuminuria which is a major indication for development of diabetic nephropathy. The prevalence of diabetic nephropathy in type 2...
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2012-05-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/1686 |
| _version_ | 1797716539769618432 |
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| author | Latifeh Abdollahi Mohammad Reza Zolfaghari Masoud Amini Rasoul Salehi |
| author_facet | Latifeh Abdollahi Mohammad Reza Zolfaghari Masoud Amini Rasoul Salehi |
| author_sort | Latifeh Abdollahi |
| collection | DOAJ |
| description | Background: Diabetes mellitus is the most prevalent metabolic disorder worldwide with many subsequent medical complications. An important complication of diabetes is microalbuminuria which is a major indication for development of diabetic nephropathy. The prevalence of diabetic nephropathy in type 2 diabetic patients has been estimated to be 47%. Since the disorder initiates with microalbuminuria, its early detection and prevention are vital. On the other hand, helicobacter pylori infection is more common in diabetic patients due to their low immune tolerance. The vacA positive genotype is reported to be effective in microalbuminuria development in type 2 diabetic patients. In this study, we investigated the relation between H. pylori vacA and microalbuminuria in type 2 diabetic patients.
Methods: This study included 88 type 2 diabetic patients among whom 60 had microalbuminuria. Stool samples were collected from all patients and DNA was extracted using QiaAmp Stool DNA Extraction Minikit. In order to detect H. pylori infection, a nested polymerase chain reaction (PCR) protocol was developed and all H. pylori positive samples were genotyped for cagA positivity.
Findings: Overall, 16 patients (18.2%) were detected to be H. pylori positive out of whom 12 had microalbuminuria (75%). VacA positivity was detected in one patient without microalbuminuria.
Conclusion: We could not establish a relationship between vacA positivity and microalbuminuria in the studied type 2 diabetic patients.
Keywords: Helicobacter pylori, VacA, Polymerase chain reaction, Type 2 diabetes, Microalbuminuria |
| first_indexed | 2024-03-12T08:22:58Z |
| format | Article |
| id | doaj.art-a7a3953c1dde4235a2435e623986f21c |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T08:22:58Z |
| publishDate | 2012-05-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-a7a3953c1dde4235a2435e623986f21c2023-09-02T18:20:31ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2012-05-01291791008The Relation between Microalbuminuria and Helicobacter Pylori VacA Gene in Type 2 Diabetic Patients, Isfahan, IranLatifeh Abdollahi0Mohammad Reza Zolfaghari1Masoud Amini2Rasoul Salehi3Department of Microbiology, Islamic Azad University, Qom Branch, Qom, IranAssistant Professor, Department of Microbiology, Islamic Azad University, Qom Branch, Qom, IranProfessor, Endocrine Research Center, Isfahan University of Medical Sciences, Isfahan, IranAssociate Professor, Department of Anatomy and Molecular Biology, School of Medicine, Pediatric Inherited Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, IranBackground: Diabetes mellitus is the most prevalent metabolic disorder worldwide with many subsequent medical complications. An important complication of diabetes is microalbuminuria which is a major indication for development of diabetic nephropathy. The prevalence of diabetic nephropathy in type 2 diabetic patients has been estimated to be 47%. Since the disorder initiates with microalbuminuria, its early detection and prevention are vital. On the other hand, helicobacter pylori infection is more common in diabetic patients due to their low immune tolerance. The vacA positive genotype is reported to be effective in microalbuminuria development in type 2 diabetic patients. In this study, we investigated the relation between H. pylori vacA and microalbuminuria in type 2 diabetic patients. Methods: This study included 88 type 2 diabetic patients among whom 60 had microalbuminuria. Stool samples were collected from all patients and DNA was extracted using QiaAmp Stool DNA Extraction Minikit. In order to detect H. pylori infection, a nested polymerase chain reaction (PCR) protocol was developed and all H. pylori positive samples were genotyped for cagA positivity. Findings: Overall, 16 patients (18.2%) were detected to be H. pylori positive out of whom 12 had microalbuminuria (75%). VacA positivity was detected in one patient without microalbuminuria. Conclusion: We could not establish a relationship between vacA positivity and microalbuminuria in the studied type 2 diabetic patients. Keywords: Helicobacter pylori, VacA, Polymerase chain reaction, Type 2 diabetes, Microalbuminuriahttp://jims.mui.ac.ir/index.php/jims/article/view/1686 |
| spellingShingle | Latifeh Abdollahi Mohammad Reza Zolfaghari Masoud Amini Rasoul Salehi The Relation between Microalbuminuria and Helicobacter Pylori VacA Gene in Type 2 Diabetic Patients, Isfahan, Iran مجله دانشکده پزشکی اصفهان |
| title | The Relation between Microalbuminuria and Helicobacter Pylori VacA Gene in Type 2 Diabetic Patients, Isfahan, Iran |
| title_full | The Relation between Microalbuminuria and Helicobacter Pylori VacA Gene in Type 2 Diabetic Patients, Isfahan, Iran |
| title_fullStr | The Relation between Microalbuminuria and Helicobacter Pylori VacA Gene in Type 2 Diabetic Patients, Isfahan, Iran |
| title_full_unstemmed | The Relation between Microalbuminuria and Helicobacter Pylori VacA Gene in Type 2 Diabetic Patients, Isfahan, Iran |
| title_short | The Relation between Microalbuminuria and Helicobacter Pylori VacA Gene in Type 2 Diabetic Patients, Isfahan, Iran |
| title_sort | relation between microalbuminuria and helicobacter pylori vaca gene in type 2 diabetic patients isfahan iran |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/1686 |
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