Chitosan-Alginate Biocomposite Containing Fucoidan for Bone Tissue Engineering
Over the last few years, significant research has been conducted in the construction of artificial bone scaffolds. In the present study, different types of polymer scaffolds, such as chitosan-alginate (Chi-Alg) and chitosan-alginate with fucoidan (Chi-Alg-fucoidan), were developed by a freeze-dryin...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2014-01-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1660-3397/12/1/300 |
| _version_ | 1828146927442591744 |
|---|---|
| author | Jayachandran Venkatesan Ira Bhatnagar Se-Kwon Kim |
| author_facet | Jayachandran Venkatesan Ira Bhatnagar Se-Kwon Kim |
| author_sort | Jayachandran Venkatesan |
| collection | DOAJ |
| description | Over the last few years, significant research has been conducted in the construction of artificial bone scaffolds. In the present study, different types of polymer scaffolds, such as chitosan-alginate (Chi-Alg) and chitosan-alginate with fucoidan (Chi-Alg-fucoidan), were developed by a freeze-drying method, and each was characterized as a bone graft substitute. The porosity, water uptake and retention ability of the prepared scaffolds showed similar efficacy. The pore size of the Chi-Alg and Chi-Alg-fucoidan scaffolds were measured from scanning electron microscopy and found to be 62–490 and 56–437 µm, respectively. In vitro studies using the MG-63 cell line revealed profound cytocompatibility, increased cell proliferation and enhanced alkaline phosphatase secretion in the Chi-Alg-fucoidan scaffold compared to the Chi-Alg scaffold. Further, protein adsorption and mineralization were about two times greater in the Chi-Alg-fucoidan scaffold than the Chi-Alg scaffold. Hence, we suggest that Chi-Alg-fucoidan will be a promising biomaterial for bone tissue regeneration. |
| first_indexed | 2024-04-11T20:53:59Z |
| format | Article |
| id | doaj.art-a7a3bd55d1714ead88fa9c4668bd40e7 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T20:53:59Z |
| publishDate | 2014-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-a7a3bd55d1714ead88fa9c4668bd40e72022-12-22T04:03:46ZengMDPI AGMarine Drugs1660-33972014-01-0112130031610.3390/md12010300md12010300Chitosan-Alginate Biocomposite Containing Fucoidan for Bone Tissue EngineeringJayachandran Venkatesan0Ira Bhatnagar1Se-Kwon Kim2Marine Bioprocess Research Center, Department of Chemistry, Pukyong National University, Busan 608-737, KoreaNanotheranostics Laboratory, Centre for Cellular and Molecular Biology, Hyderabad 500-007, IndiaMarine Bioprocess Research Center, Department of Chemistry, Pukyong National University, Busan 608-737, KoreaOver the last few years, significant research has been conducted in the construction of artificial bone scaffolds. In the present study, different types of polymer scaffolds, such as chitosan-alginate (Chi-Alg) and chitosan-alginate with fucoidan (Chi-Alg-fucoidan), were developed by a freeze-drying method, and each was characterized as a bone graft substitute. The porosity, water uptake and retention ability of the prepared scaffolds showed similar efficacy. The pore size of the Chi-Alg and Chi-Alg-fucoidan scaffolds were measured from scanning electron microscopy and found to be 62–490 and 56–437 µm, respectively. In vitro studies using the MG-63 cell line revealed profound cytocompatibility, increased cell proliferation and enhanced alkaline phosphatase secretion in the Chi-Alg-fucoidan scaffold compared to the Chi-Alg scaffold. Further, protein adsorption and mineralization were about two times greater in the Chi-Alg-fucoidan scaffold than the Chi-Alg scaffold. Hence, we suggest that Chi-Alg-fucoidan will be a promising biomaterial for bone tissue regeneration.http://www.mdpi.com/1660-3397/12/1/300chitosanalginatefucoidanbone tissue engineeringbiomaterials |
| spellingShingle | Jayachandran Venkatesan Ira Bhatnagar Se-Kwon Kim Chitosan-Alginate Biocomposite Containing Fucoidan for Bone Tissue Engineering Marine Drugs chitosan alginate fucoidan bone tissue engineering biomaterials |
| title | Chitosan-Alginate Biocomposite Containing Fucoidan for Bone Tissue Engineering |
| title_full | Chitosan-Alginate Biocomposite Containing Fucoidan for Bone Tissue Engineering |
| title_fullStr | Chitosan-Alginate Biocomposite Containing Fucoidan for Bone Tissue Engineering |
| title_full_unstemmed | Chitosan-Alginate Biocomposite Containing Fucoidan for Bone Tissue Engineering |
| title_short | Chitosan-Alginate Biocomposite Containing Fucoidan for Bone Tissue Engineering |
| title_sort | chitosan alginate biocomposite containing fucoidan for bone tissue engineering |
| topic | chitosan alginate fucoidan bone tissue engineering biomaterials |
| url | http://www.mdpi.com/1660-3397/12/1/300 |
| work_keys_str_mv | AT jayachandranvenkatesan chitosanalginatebiocompositecontainingfucoidanforbonetissueengineering AT irabhatnagar chitosanalginatebiocompositecontainingfucoidanforbonetissueengineering AT sekwonkim chitosanalginatebiocompositecontainingfucoidanforbonetissueengineering |