Wound healing activity of Ullucus tuberosus, an Andean tuber crop
Objective: This study was designed to investigate the wound healing activity of aqueous extracts of Ullucus tuberosus (U. tuberosus) using in vitro models. Methods: Lyophilized pulp and acetone extracts of U. tuberosus were produced using ultrasound extraction. The capacity for collagenase activatio...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-06-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169116309303 |
| _version_ | 1818426976759709696 |
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| author | Nathalie Heil Karent Bravo Andrés Montoya Sara Robledo Edison Osorio |
| author_facet | Nathalie Heil Karent Bravo Andrés Montoya Sara Robledo Edison Osorio |
| author_sort | Nathalie Heil |
| collection | DOAJ |
| description | Objective: This study was designed to investigate the wound healing activity of aqueous extracts of Ullucus tuberosus (U. tuberosus) using in vitro models.
Methods: Lyophilized pulp and acetone extracts of U. tuberosus were produced using ultrasound extraction. The capacity for collagenase activation was evaluated using fluorescence detection of the enzymatic activity. Then, the influence of U. tuberosus extracts on cell proliferation, cell migration and synthesis of the extracellular matrix (ECM) proteins, metalloproteinase (MMP-1) and pro-collagen was analyzed using human dermal fibroblasts in culture.
Results: An increase in collagenase activity of 12% supports the utility of U. tuberosus as an agent for scar treatment. In addition, the extracts showed an increase in the proliferation and migration of human dermal fibroblasts and the production of pro-collagen and MMP-1 after treatment with U. tuberosus extracts. The increase in proliferation, migration and pro-collagen levels positively influenced the regeneration of scarless tissue during the proliferation phase, whereas the increase in MMP-1 may have favored the wound healing process during the remodeling and cellular differentiation phases.
Conclusion: The results of this study show for first time that U. tuberosus is a promising candidate to support scarless tissue regeneration. |
| first_indexed | 2024-12-14T14:38:23Z |
| format | Article |
| id | doaj.art-a7a443acbc1843f4946fd2d656dd10ad |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-14T14:38:23Z |
| publishDate | 2017-06-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-a7a443acbc1843f4946fd2d656dd10ad2022-12-21T22:57:29ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912017-06-017653854310.1016/j.apjtb.2017.05.007Wound healing activity of Ullucus tuberosus, an Andean tuber cropNathalie Heil0Karent Bravo1Andrés Montoya2Sara Robledo3Edison Osorio4Grupo de Investigación en Sustancias Bioactivas, Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, ColombiaGrupo de Investigación en Sustancias Bioactivas, Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, ColombiaPECET – Medical Research Institute, School of Medicine, University of Antioquia, Calle 70 No. 52-21, Medellín, ColombiaPECET – Medical Research Institute, School of Medicine, University of Antioquia, Calle 70 No. 52-21, Medellín, ColombiaGrupo de Investigación en Sustancias Bioactivas, Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, ColombiaObjective: This study was designed to investigate the wound healing activity of aqueous extracts of Ullucus tuberosus (U. tuberosus) using in vitro models. Methods: Lyophilized pulp and acetone extracts of U. tuberosus were produced using ultrasound extraction. The capacity for collagenase activation was evaluated using fluorescence detection of the enzymatic activity. Then, the influence of U. tuberosus extracts on cell proliferation, cell migration and synthesis of the extracellular matrix (ECM) proteins, metalloproteinase (MMP-1) and pro-collagen was analyzed using human dermal fibroblasts in culture. Results: An increase in collagenase activity of 12% supports the utility of U. tuberosus as an agent for scar treatment. In addition, the extracts showed an increase in the proliferation and migration of human dermal fibroblasts and the production of pro-collagen and MMP-1 after treatment with U. tuberosus extracts. The increase in proliferation, migration and pro-collagen levels positively influenced the regeneration of scarless tissue during the proliferation phase, whereas the increase in MMP-1 may have favored the wound healing process during the remodeling and cellular differentiation phases. Conclusion: The results of this study show for first time that U. tuberosus is a promising candidate to support scarless tissue regeneration.http://www.sciencedirect.com/science/article/pii/S2221169116309303Wound healingCollagenasePro-collagenMMP-1Ullucus tuberosus |
| spellingShingle | Nathalie Heil Karent Bravo Andrés Montoya Sara Robledo Edison Osorio Wound healing activity of Ullucus tuberosus, an Andean tuber crop Asian Pacific Journal of Tropical Biomedicine Wound healing Collagenase Pro-collagen MMP-1 Ullucus tuberosus |
| title | Wound healing activity of Ullucus tuberosus, an Andean tuber crop |
| title_full | Wound healing activity of Ullucus tuberosus, an Andean tuber crop |
| title_fullStr | Wound healing activity of Ullucus tuberosus, an Andean tuber crop |
| title_full_unstemmed | Wound healing activity of Ullucus tuberosus, an Andean tuber crop |
| title_short | Wound healing activity of Ullucus tuberosus, an Andean tuber crop |
| title_sort | wound healing activity of ullucus tuberosus an andean tuber crop |
| topic | Wound healing Collagenase Pro-collagen MMP-1 Ullucus tuberosus |
| url | http://www.sciencedirect.com/science/article/pii/S2221169116309303 |
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