Transgene expression and Bt protein content in transgenic Bt maize (MON810) under optimal and stressful environmental conditions.
Bt protein content in transgenic insect resistant (Bt) maize may vary between tissues within plants and between plants growing under different environmental conditions. However, it is unknown whether and how Bt protein content correlates with transgene expression, and whether this relationship is in...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4390241?pdf=render |
| _version_ | 1811263706413137920 |
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| author | Miluse Trtikova Odd Gunnar Wikmark Niklaus Zemp Alex Widmer Angelika Hilbeck |
| author_facet | Miluse Trtikova Odd Gunnar Wikmark Niklaus Zemp Alex Widmer Angelika Hilbeck |
| author_sort | Miluse Trtikova |
| collection | DOAJ |
| description | Bt protein content in transgenic insect resistant (Bt) maize may vary between tissues within plants and between plants growing under different environmental conditions. However, it is unknown whether and how Bt protein content correlates with transgene expression, and whether this relationship is influenced by stressful environmental conditions. Two Bt maize varieties containing the same transgene cassette (MON 810) were grown under optimal and stressful conditions. Before and during stress exposure, the upper leaves were analysed for transgene expression using quantitative RT-PCR and for Bt content using ELISA. Under optimal conditions there was no significant difference in the transgene expression between the two investigated Bt maize varieties whereas Bt protein content differed significantly. Transgene expression was correlated with Bt protein content in only one of the varieties. Under stressful environmental conditions we found similar transgene expressions as under optimal conditions but Bt content responded differently. These results suggest that Bt content is not only controlled by the transgene expression but is also dependent on the genetic background of the maize variety. Under stressful conditions the concentration of Bt protein is even more difficult to predict. |
| first_indexed | 2024-04-12T19:50:01Z |
| format | Article |
| id | doaj.art-a7a4dfd466834c478640bbbec3f3cd15 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T19:50:01Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-a7a4dfd466834c478640bbbec3f3cd152022-12-22T03:18:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01104e012301110.1371/journal.pone.0123011Transgene expression and Bt protein content in transgenic Bt maize (MON810) under optimal and stressful environmental conditions.Miluse TrtikovaOdd Gunnar WikmarkNiklaus ZempAlex WidmerAngelika HilbeckBt protein content in transgenic insect resistant (Bt) maize may vary between tissues within plants and between plants growing under different environmental conditions. However, it is unknown whether and how Bt protein content correlates with transgene expression, and whether this relationship is influenced by stressful environmental conditions. Two Bt maize varieties containing the same transgene cassette (MON 810) were grown under optimal and stressful conditions. Before and during stress exposure, the upper leaves were analysed for transgene expression using quantitative RT-PCR and for Bt content using ELISA. Under optimal conditions there was no significant difference in the transgene expression between the two investigated Bt maize varieties whereas Bt protein content differed significantly. Transgene expression was correlated with Bt protein content in only one of the varieties. Under stressful environmental conditions we found similar transgene expressions as under optimal conditions but Bt content responded differently. These results suggest that Bt content is not only controlled by the transgene expression but is also dependent on the genetic background of the maize variety. Under stressful conditions the concentration of Bt protein is even more difficult to predict.http://europepmc.org/articles/PMC4390241?pdf=render |
| spellingShingle | Miluse Trtikova Odd Gunnar Wikmark Niklaus Zemp Alex Widmer Angelika Hilbeck Transgene expression and Bt protein content in transgenic Bt maize (MON810) under optimal and stressful environmental conditions. PLoS ONE |
| title | Transgene expression and Bt protein content in transgenic Bt maize (MON810) under optimal and stressful environmental conditions. |
| title_full | Transgene expression and Bt protein content in transgenic Bt maize (MON810) under optimal and stressful environmental conditions. |
| title_fullStr | Transgene expression and Bt protein content in transgenic Bt maize (MON810) under optimal and stressful environmental conditions. |
| title_full_unstemmed | Transgene expression and Bt protein content in transgenic Bt maize (MON810) under optimal and stressful environmental conditions. |
| title_short | Transgene expression and Bt protein content in transgenic Bt maize (MON810) under optimal and stressful environmental conditions. |
| title_sort | transgene expression and bt protein content in transgenic bt maize mon810 under optimal and stressful environmental conditions |
| url | http://europepmc.org/articles/PMC4390241?pdf=render |
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