A succession of pulmonary microbiota in broilers during the growth cycle
ABSTRACT: Respiratory health problems in poultry production are frequent and knotty and thus attract the attention of farmers and researchers. The breakthrough of gene sequencing technology has revealed that healthy lungs harbor rich microbiota, whose succession and homeostasis are closely related t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123004030 |
| _version_ | 1797739013836111872 |
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| author | Dan Shen Kai Wang Mohamed Ahmed Fathi Yansen Li Tin-Tin Win-Shwe Chunmei Li |
| author_facet | Dan Shen Kai Wang Mohamed Ahmed Fathi Yansen Li Tin-Tin Win-Shwe Chunmei Li |
| author_sort | Dan Shen |
| collection | DOAJ |
| description | ABSTRACT: Respiratory health problems in poultry production are frequent and knotty and thus attract the attention of farmers and researchers. The breakthrough of gene sequencing technology has revealed that healthy lungs harbor rich microbiota, whose succession and homeostasis are closely related to lung health status, suggesting a new idea to explore the mechanism of lung injury in broilers with pulmonary microbiota as the entry point. This study aimed to investigate the succession of pulmonary microbiota in healthy broilers during the growth cycle. Fixed and molecular samples were collected from the lungs of healthy broilers at 1, 3, 14, 21, 28, and 42 d of age. Lung tissue morphology was observed by hematoxylin and eosin staining, and the changes in the composition and diversity of pulmonary microbiota were analyzed using 16S rRNA gene sequencing. The results showed that lung index peaked at 3 d, then decreased with age. No significant change was observed in the α diversity of pulmonary microbiota, while the β diversity changed regularly with age during the broilers’ growth cycle. The relative abundance of dominant bacteria of Firmicutes and their subordinate Lactobacillus increased with age, while the abundance of Proteobacteria decreased with age. The correlation analysis between the abundance of differential bacteria and predicted function showed that dominant bacteria of Firmicutes, Proteobacteria and Lactobacillus were significantly correlated with most functional abundance, indicating that they may involve in lung functional development and physiological activities of broilers. Collectively, these findings suggest that the lung has been colonized with abundant microbiota in broilers when they were just hatched, and their composition changed regularly with day age. The dominant bacteria, Firmicutes, Proteobacteria, and Lactobacillus, play crucial roles in lung function development and physiological activities. It paves the way for further research on the mechanism of pulmonary microbiota-mediated lung injury in broilers. |
| first_indexed | 2024-03-12T13:51:14Z |
| format | Article |
| id | doaj.art-a7a566e25f6e4ca39d2a4f07f2a3ecde |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-12T13:51:14Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-a7a566e25f6e4ca39d2a4f07f2a3ecde2023-08-23T04:32:40ZengElsevierPoultry Science0032-57912023-09-011029102884A succession of pulmonary microbiota in broilers during the growth cycleDan Shen0Kai Wang1Mohamed Ahmed Fathi2Yansen Li3Tin-Tin Win-Shwe4Chunmei Li5Research Centre for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaResearch Centre for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaResearch Centre for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; Animal Production Research Institute, Agricultural Research Centre, Dokki, Giza 12618, EgyptResearch Centre for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaHealth and Environmental Risk Division, National Institute for Environmental Studies, Tsukuba 305-8506, JapanResearch Centre for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; Corresponding author:ABSTRACT: Respiratory health problems in poultry production are frequent and knotty and thus attract the attention of farmers and researchers. The breakthrough of gene sequencing technology has revealed that healthy lungs harbor rich microbiota, whose succession and homeostasis are closely related to lung health status, suggesting a new idea to explore the mechanism of lung injury in broilers with pulmonary microbiota as the entry point. This study aimed to investigate the succession of pulmonary microbiota in healthy broilers during the growth cycle. Fixed and molecular samples were collected from the lungs of healthy broilers at 1, 3, 14, 21, 28, and 42 d of age. Lung tissue morphology was observed by hematoxylin and eosin staining, and the changes in the composition and diversity of pulmonary microbiota were analyzed using 16S rRNA gene sequencing. The results showed that lung index peaked at 3 d, then decreased with age. No significant change was observed in the α diversity of pulmonary microbiota, while the β diversity changed regularly with age during the broilers’ growth cycle. The relative abundance of dominant bacteria of Firmicutes and their subordinate Lactobacillus increased with age, while the abundance of Proteobacteria decreased with age. The correlation analysis between the abundance of differential bacteria and predicted function showed that dominant bacteria of Firmicutes, Proteobacteria and Lactobacillus were significantly correlated with most functional abundance, indicating that they may involve in lung functional development and physiological activities of broilers. Collectively, these findings suggest that the lung has been colonized with abundant microbiota in broilers when they were just hatched, and their composition changed regularly with day age. The dominant bacteria, Firmicutes, Proteobacteria, and Lactobacillus, play crucial roles in lung function development and physiological activities. It paves the way for further research on the mechanism of pulmonary microbiota-mediated lung injury in broilers.http://www.sciencedirect.com/science/article/pii/S0032579123004030pulmonary microbiotasuccessionbroilerlung health |
| spellingShingle | Dan Shen Kai Wang Mohamed Ahmed Fathi Yansen Li Tin-Tin Win-Shwe Chunmei Li A succession of pulmonary microbiota in broilers during the growth cycle Poultry Science pulmonary microbiota succession broiler lung health |
| title | A succession of pulmonary microbiota in broilers during the growth cycle |
| title_full | A succession of pulmonary microbiota in broilers during the growth cycle |
| title_fullStr | A succession of pulmonary microbiota in broilers during the growth cycle |
| title_full_unstemmed | A succession of pulmonary microbiota in broilers during the growth cycle |
| title_short | A succession of pulmonary microbiota in broilers during the growth cycle |
| title_sort | succession of pulmonary microbiota in broilers during the growth cycle |
| topic | pulmonary microbiota succession broiler lung health |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123004030 |
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