Secretome Proteomic Approaches for Biomarker Discovery: An Update on Colorectal Cancer
Searching for new cancer-related biomarkers is a key priority for the early detection of solid tumors, such as colorectal cancer (CRC), in clinically relevant biological fluids. The cell line and/or tumor tissue secretome represents a valuable resource for discovering novel protein markers secreted...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1010-660X/56/9/443 |
| _version_ | 1797721145752944640 |
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| author | Armando Cevenini Stefania Orrù Esther Imperlini |
| author_facet | Armando Cevenini Stefania Orrù Esther Imperlini |
| author_sort | Armando Cevenini |
| collection | DOAJ |
| description | Searching for new cancer-related biomarkers is a key priority for the early detection of solid tumors, such as colorectal cancer (CRC), in clinically relevant biological fluids. The cell line and/or tumor tissue secretome represents a valuable resource for discovering novel protein markers secreted by cancer cells. The advantage of a secretome analysis is the reduction of the large dynamic range characterizing human plasma/serum, and the simultaneous enrichment of low abundance cancer-secreted proteins, thereby overcoming the technical limitations underlying the direct search in blood samples. In this review, we provided a comprehensive overview of recent studies on the CRC secretome for biomarker discovery, focusing both on methodological and technical aspects of secretome proteomic approaches and on biomarker-independent validation in CRC patient samples (blood and tissues). Secretome proteomics are mainly based on LC-MS/MS analyses for which secretome samples are either in-gel or in-solution trypsin-digested. Adequate numbers of biological and technical replicates are required to ensure high reproducibility and robustness of the secretome studies. Moreover, another major challenge is the accuracy of proteomic quantitative analysis performed by label-free or labeling methods. The analysis of differentially expressed proteins in the CRC secretome by using bioinformatic tools allowed the identification of potential biomarkers for early CRC detection. In this scenario, this review may help to follow-up the recent secretome studies in order to select promising circulating biomarkers to be validated in larger screenings, thereby contributing toward a complete translation in clinical practice. |
| first_indexed | 2024-03-12T09:29:26Z |
| format | Article |
| id | doaj.art-a7a6f593e64d4413949c85ea42f0681c |
| institution | Directory Open Access Journal |
| issn | 1010-660X |
| language | English |
| last_indexed | 2024-03-12T09:29:26Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj.art-a7a6f593e64d4413949c85ea42f0681c2023-09-02T13:56:23ZengMDPI AGMedicina1010-660X2020-08-015644344310.3390/medicina56090443Secretome Proteomic Approaches for Biomarker Discovery: An Update on Colorectal CancerArmando Cevenini0Stefania Orrù1Esther Imperlini2Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli “Federico II”, 80131 Napoli, ItalyCEINGE-Biotecnologie Avanzate S.c.a r.l., 80145 Napoli, ItalyIRCCS SDN, 80143 Napoli, ItalySearching for new cancer-related biomarkers is a key priority for the early detection of solid tumors, such as colorectal cancer (CRC), in clinically relevant biological fluids. The cell line and/or tumor tissue secretome represents a valuable resource for discovering novel protein markers secreted by cancer cells. The advantage of a secretome analysis is the reduction of the large dynamic range characterizing human plasma/serum, and the simultaneous enrichment of low abundance cancer-secreted proteins, thereby overcoming the technical limitations underlying the direct search in blood samples. In this review, we provided a comprehensive overview of recent studies on the CRC secretome for biomarker discovery, focusing both on methodological and technical aspects of secretome proteomic approaches and on biomarker-independent validation in CRC patient samples (blood and tissues). Secretome proteomics are mainly based on LC-MS/MS analyses for which secretome samples are either in-gel or in-solution trypsin-digested. Adequate numbers of biological and technical replicates are required to ensure high reproducibility and robustness of the secretome studies. Moreover, another major challenge is the accuracy of proteomic quantitative analysis performed by label-free or labeling methods. The analysis of differentially expressed proteins in the CRC secretome by using bioinformatic tools allowed the identification of potential biomarkers for early CRC detection. In this scenario, this review may help to follow-up the recent secretome studies in order to select promising circulating biomarkers to be validated in larger screenings, thereby contributing toward a complete translation in clinical practice.https://www.mdpi.com/1010-660X/56/9/443secretomecolorectal cancercancer cell linesbiomarkerproteomicsmass spectrometry |
| spellingShingle | Armando Cevenini Stefania Orrù Esther Imperlini Secretome Proteomic Approaches for Biomarker Discovery: An Update on Colorectal Cancer Medicina secretome colorectal cancer cancer cell lines biomarker proteomics mass spectrometry |
| title | Secretome Proteomic Approaches for Biomarker Discovery: An Update on Colorectal Cancer |
| title_full | Secretome Proteomic Approaches for Biomarker Discovery: An Update on Colorectal Cancer |
| title_fullStr | Secretome Proteomic Approaches for Biomarker Discovery: An Update on Colorectal Cancer |
| title_full_unstemmed | Secretome Proteomic Approaches for Biomarker Discovery: An Update on Colorectal Cancer |
| title_short | Secretome Proteomic Approaches for Biomarker Discovery: An Update on Colorectal Cancer |
| title_sort | secretome proteomic approaches for biomarker discovery an update on colorectal cancer |
| topic | secretome colorectal cancer cancer cell lines biomarker proteomics mass spectrometry |
| url | https://www.mdpi.com/1010-660X/56/9/443 |
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