Prevalence and antimicrobial resistance of Staphylococcus species isolated from cats and dogs
Background: Methicillin-resistant staphylococci (MRS) are an emerging global problem with serious public health concerns. Aims: This study investigated the prevalence and antimicrobial susceptibility of commensal Staphylococcus species isolated from healthy and clinical cats and dogs. Methods: N...
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| Format: | Article |
| Language: | English |
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Tripoli University
2020-12-01
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| Series: | Open Veterinary Journal |
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| Online Access: | https://www.openveterinaryjournal.com/OVJ-2020-09-302%20H.R.%20Elnageh%20et%20al.pdf |
| _version_ | 1818677031231029248 |
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| author | Hiam R. Elnageh Murad A. Hiblu Mohamed Salah Abbassi Yousef M. Abouzeed Mohamed O. Ahmed |
| author_facet | Hiam R. Elnageh Murad A. Hiblu Mohamed Salah Abbassi Yousef M. Abouzeed Mohamed O. Ahmed |
| author_sort | Hiam R. Elnageh |
| collection | DOAJ |
| description | Background: Methicillin-resistant staphylococci (MRS) are an emerging global problem with serious public health concerns.
Aims: This study investigated the prevalence and antimicrobial susceptibility of commensal Staphylococcus species isolated from healthy and clinical cats and dogs.
Methods: Nasal swab samples were collected from animals and processed using selective and semi-selective mediums. Presumptive isolates were subjected to biochemical testing and analysed using the Phoenix automated identification and susceptibility testing system. PCRs protocols were used to screen for mecA and pvl genes.
Results: In total, 151 pets (103 cats, 48 dogs) were enrolled of which 14 dogs (29%) and 24 cats (23%) were colonized with various Staphylococcus species mainly originated from healthy animals. A total of 38 staphylococci isolates were collected distributed between 24 coagulase-negative and 14 coagulase-positive staphylococci. Only 13 staphylococci strains were identified as MRS of which only five isolates expressed the mecA gene exclusively originated from healthy pets.
Conclusion: This is the first study reporting the prevalence and colonization status of staphylococci species and MRS strains isolated from cats and dogs in Libya. The study reports important information of medical and clinical importance on antimicrobial and multidrug resistance of different staphylococci strains particularly the coagulase negative species. |
| first_indexed | 2024-12-17T08:52:54Z |
| format | Article |
| id | doaj.art-a7a7d1400ff848889d4abf9169447677 |
| institution | Directory Open Access Journal |
| issn | 2218-6050 2218-6050 |
| language | English |
| last_indexed | 2024-12-17T08:52:54Z |
| publishDate | 2020-12-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj.art-a7a7d1400ff848889d4abf91694476772022-12-21T21:56:01ZengTripoli UniversityOpen Veterinary Journal2218-60502218-60502020-12-01104452456http://dx.doi.org/10.4314/ovj.v10i4.13Prevalence and antimicrobial resistance of Staphylococcus species isolated from cats and dogsHiam R. Elnageh0Murad A. Hiblu1Mohamed Salah Abbassi2Yousef M. Abouzeed3Mohamed O. Ahmed4Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Tripoli, Tripoli, LibyaDepartment of Internal Medicine, Faculty of Veterinary Medicine, University of Tripoli, Tripoli, LibyaUniversity of Tunis El Manar, Institute of Veterinary Research of Tunisia, 20 street Jebel Lakhdhar, Bab Saadoun, Tunis 1006, TunisiaDepartment of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Tripoli, Tripoli, LibyaDepartment of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Tripoli, Tripoli, LibyaBackground: Methicillin-resistant staphylococci (MRS) are an emerging global problem with serious public health concerns. Aims: This study investigated the prevalence and antimicrobial susceptibility of commensal Staphylococcus species isolated from healthy and clinical cats and dogs. Methods: Nasal swab samples were collected from animals and processed using selective and semi-selective mediums. Presumptive isolates were subjected to biochemical testing and analysed using the Phoenix automated identification and susceptibility testing system. PCRs protocols were used to screen for mecA and pvl genes. Results: In total, 151 pets (103 cats, 48 dogs) were enrolled of which 14 dogs (29%) and 24 cats (23%) were colonized with various Staphylococcus species mainly originated from healthy animals. A total of 38 staphylococci isolates were collected distributed between 24 coagulase-negative and 14 coagulase-positive staphylococci. Only 13 staphylococci strains were identified as MRS of which only five isolates expressed the mecA gene exclusively originated from healthy pets. Conclusion: This is the first study reporting the prevalence and colonization status of staphylococci species and MRS strains isolated from cats and dogs in Libya. The study reports important information of medical and clinical importance on antimicrobial and multidrug resistance of different staphylococci strains particularly the coagulase negative species.https://www.openveterinaryjournal.com/OVJ-2020-09-302%20H.R.%20Elnageh%20et%20al.pdfcoagulase negative staphylococcilibyameca genepet animalsstaphylococcus |
| spellingShingle | Hiam R. Elnageh Murad A. Hiblu Mohamed Salah Abbassi Yousef M. Abouzeed Mohamed O. Ahmed Prevalence and antimicrobial resistance of Staphylococcus species isolated from cats and dogs Open Veterinary Journal coagulase negative staphylococci libya meca gene pet animals staphylococcus |
| title | Prevalence and antimicrobial resistance of Staphylococcus species isolated from cats and dogs |
| title_full | Prevalence and antimicrobial resistance of Staphylococcus species isolated from cats and dogs |
| title_fullStr | Prevalence and antimicrobial resistance of Staphylococcus species isolated from cats and dogs |
| title_full_unstemmed | Prevalence and antimicrobial resistance of Staphylococcus species isolated from cats and dogs |
| title_short | Prevalence and antimicrobial resistance of Staphylococcus species isolated from cats and dogs |
| title_sort | prevalence and antimicrobial resistance of staphylococcus species isolated from cats and dogs |
| topic | coagulase negative staphylococci libya meca gene pet animals staphylococcus |
| url | https://www.openveterinaryjournal.com/OVJ-2020-09-302%20H.R.%20Elnageh%20et%20al.pdf |
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