INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF <i>Eucalyptus benthamii</i> X <i>Eucalyptus dunnii</i>
<p><a>http://dx.doi.org/10.5902/1980509814572</a></p><p class="Pa4">The aims of this research were to evaluate different culture media for indirect organogenesis and shoot multiplication of <em>Eucalyptus benthamii </em>x <em>Eucalyptus dunni...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Portuguese |
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Universidade Federal de Santa Maria
2014-06-01
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| Series: | Ciência Florestal |
| Subjects: | |
| Online Access: | http://cascavel.ufsm.br/revistas/ojs-2.2.2/index.php/cienciaflorestal/article/view/14572 |
| _version_ | 1818979953393270784 |
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| author | Yohana de Oliveira-Cauduro Laís Gomes Adamuchio Juliana Degenhardt-Goldbach João Carlos Bespalhok Filho Roberson Dibax Marguerite Quoirin |
| author_facet | Yohana de Oliveira-Cauduro Laís Gomes Adamuchio Juliana Degenhardt-Goldbach João Carlos Bespalhok Filho Roberson Dibax Marguerite Quoirin |
| author_sort | Yohana de Oliveira-Cauduro |
| collection | DOAJ |
| description | <p><a>http://dx.doi.org/10.5902/1980509814572</a></p><p class="Pa4">The aims of this research were to evaluate different culture media for indirect organogenesis and shoot multiplication of <em>Eucalyptus benthamii </em>x <em>Eucalyptus dunnii</em>. For organogenesis, leaf explants were used to test the following treatments: two culture media (MS N/2 and JADS) supplemented with 0.1 μM 1-naphthaleneacetic acid (NAA) and thidiazuron (TDZ) (0.1 or 0.5 μM), with or without PVP- 40 (250 mg L-1). The percentage of oxidized explants, callus forming explants, explants with anthocyanin, buds, shoots and the shoot number per explant were evaluated. In the multiplication experiment, isolated shoots were cultivated in MS, JADS and WPM media, all supplemented with 1.11 μM BAP. Four subcultures were carried out every 28 days. In every subculture the explant oxidation, partial or total leaf chlorosis, fresh mass and mean number of shoot per explant were evaluated. The MS N/2 medium supplemented with 0.1 μM NAA and 0.5 μM TDZ promoted the highest rate of organogenesis (8.3%) and the culture media MS supplemented with 1.11 μM BAP the multiplication rate was higher than in the other media, in the first and the second subcultures (9.28 and 9.24, respectively), without differences between the three media in the following subcultures.</p><p> </p> |
| first_indexed | 2024-12-20T17:07:43Z |
| format | Article |
| id | doaj.art-a7a9c4d1c29a40a8b4bb242b53cfa3bd |
| institution | Directory Open Access Journal |
| issn | 0103-9954 1980-5098 |
| language | Portuguese |
| last_indexed | 2024-12-20T17:07:43Z |
| publishDate | 2014-06-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Florestal |
| spelling | doaj.art-a7a9c4d1c29a40a8b4bb242b53cfa3bd2022-12-21T19:32:13ZporUniversidade Federal de Santa MariaCiência Florestal0103-99541980-50982014-06-0124234735510.5902/19805098145728030INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF <i>Eucalyptus benthamii</i> X <i>Eucalyptus dunnii</i>Yohana de Oliveira-CauduroLaís Gomes AdamuchioJuliana Degenhardt-GoldbachJoão Carlos Bespalhok FilhoRoberson DibaxMarguerite Quoirin<p><a>http://dx.doi.org/10.5902/1980509814572</a></p><p class="Pa4">The aims of this research were to evaluate different culture media for indirect organogenesis and shoot multiplication of <em>Eucalyptus benthamii </em>x <em>Eucalyptus dunnii</em>. For organogenesis, leaf explants were used to test the following treatments: two culture media (MS N/2 and JADS) supplemented with 0.1 μM 1-naphthaleneacetic acid (NAA) and thidiazuron (TDZ) (0.1 or 0.5 μM), with or without PVP- 40 (250 mg L-1). The percentage of oxidized explants, callus forming explants, explants with anthocyanin, buds, shoots and the shoot number per explant were evaluated. In the multiplication experiment, isolated shoots were cultivated in MS, JADS and WPM media, all supplemented with 1.11 μM BAP. Four subcultures were carried out every 28 days. In every subculture the explant oxidation, partial or total leaf chlorosis, fresh mass and mean number of shoot per explant were evaluated. The MS N/2 medium supplemented with 0.1 μM NAA and 0.5 μM TDZ promoted the highest rate of organogenesis (8.3%) and the culture media MS supplemented with 1.11 μM BAP the multiplication rate was higher than in the other media, in the first and the second subcultures (9.28 and 9.24, respectively), without differences between the three media in the following subcultures.</p><p> </p>http://cascavel.ufsm.br/revistas/ojs-2.2.2/index.php/cienciaflorestal/article/view/14572regeneraçãoespécie lenhosaregulador vegetalmeio de cultura. |
| spellingShingle | Yohana de Oliveira-Cauduro Laís Gomes Adamuchio Juliana Degenhardt-Goldbach João Carlos Bespalhok Filho Roberson Dibax Marguerite Quoirin INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF <i>Eucalyptus benthamii</i> X <i>Eucalyptus dunnii</i> Ciência Florestal regeneração espécie lenhosa regulador vegetal meio de cultura. |
| title | INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF <i>Eucalyptus benthamii</i> X <i>Eucalyptus dunnii</i> |
| title_full | INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF <i>Eucalyptus benthamii</i> X <i>Eucalyptus dunnii</i> |
| title_fullStr | INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF <i>Eucalyptus benthamii</i> X <i>Eucalyptus dunnii</i> |
| title_full_unstemmed | INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF <i>Eucalyptus benthamii</i> X <i>Eucalyptus dunnii</i> |
| title_short | INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF <i>Eucalyptus benthamii</i> X <i>Eucalyptus dunnii</i> |
| title_sort | indirect organogenesis from leaf explants and in vitro shoots multiplication of i eucalyptus benthamii i x i eucalyptus dunnii i |
| topic | regeneração espécie lenhosa regulador vegetal meio de cultura. |
| url | http://cascavel.ufsm.br/revistas/ojs-2.2.2/index.php/cienciaflorestal/article/view/14572 |
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