Endophytic Penicillium species secretes mycophenolic acid that inhibits the growth of phytopathogenic fungi
Abstract The worldwide demand for reduced and restricted use of pesticides in agriculture due to serious environmental effects, health risks and the development of pathogen resistance calls for the discovery of new bioactive compounds. In the medical field, antibiotic‐resistant microorganisms have b...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-08-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.14203 |
| _version_ | 1797773491852804096 |
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| author | Neri Azar Orna Liarzi Maor Zavitan Mohamed Samara Ahmed Nasser David Ezra |
| author_facet | Neri Azar Orna Liarzi Maor Zavitan Mohamed Samara Ahmed Nasser David Ezra |
| author_sort | Neri Azar |
| collection | DOAJ |
| description | Abstract The worldwide demand for reduced and restricted use of pesticides in agriculture due to serious environmental effects, health risks and the development of pathogen resistance calls for the discovery of new bioactive compounds. In the medical field, antibiotic‐resistant microorganisms have become a major threat to man, increasing mortality. Endophytes are endosymbiotic microorganisms that inhabit plant tissues without causing any visible damage to their host. Many endophytes secrete secondary metabolites with biological activity against a broad range of pathogens, making them potential candidates for novel drugs and alternative pesticides of natural origin. We isolated endophytes from wild plants in Israel, focusing on endophytes that secrete secondary metabolites with biological activity. We isolated 302 different endophytes from 30 different wild plants; 70 of them exhibited biological activity against phytopathogens. One biologically active fungal endophyte from the genus Penicillium, isolated from a squill (Urginea maritima) leaf, was further examined. Chloroform‐based extraction of its growth medium was similarly active against phytopathogens. High‐performance liquid chromatography separation followed by gas chromatography/mass spectrometry analysis revealed a single compound—mycophenolic acid—as the main contributor to the biological activity of the organic extract. |
| first_indexed | 2024-03-12T22:07:19Z |
| format | Article |
| id | doaj.art-a7aa9210822144cdb8fa2f56268e43e5 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-03-12T22:07:19Z |
| publishDate | 2023-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-a7aa9210822144cdb8fa2f56268e43e52023-07-24T10:31:47ZengWileyMicrobial Biotechnology1751-79152023-08-011681629163810.1111/1751-7915.14203Endophytic Penicillium species secretes mycophenolic acid that inhibits the growth of phytopathogenic fungiNeri Azar0Orna Liarzi1Maor Zavitan2Mohamed Samara3Ahmed Nasser4David Ezra5Department of Plant Pathology and Weed Research ARO – the Volcani Center Rishon LeZion IsraelDepartment of Plant Pathology and Weed Research ARO – the Volcani Center Rishon LeZion IsraelDepartment of Plant Pathology and Weed Research ARO – the Volcani Center Rishon LeZion IsraelInstitute of Soils, Water and Environmental Sciences ARO – the Volcani Center Rishon LeZion IsraelInstitute of Soils, Water and Environmental Sciences ARO – the Volcani Center Rishon LeZion IsraelDepartment of Plant Pathology and Weed Research ARO – the Volcani Center Rishon LeZion IsraelAbstract The worldwide demand for reduced and restricted use of pesticides in agriculture due to serious environmental effects, health risks and the development of pathogen resistance calls for the discovery of new bioactive compounds. In the medical field, antibiotic‐resistant microorganisms have become a major threat to man, increasing mortality. Endophytes are endosymbiotic microorganisms that inhabit plant tissues without causing any visible damage to their host. Many endophytes secrete secondary metabolites with biological activity against a broad range of pathogens, making them potential candidates for novel drugs and alternative pesticides of natural origin. We isolated endophytes from wild plants in Israel, focusing on endophytes that secrete secondary metabolites with biological activity. We isolated 302 different endophytes from 30 different wild plants; 70 of them exhibited biological activity against phytopathogens. One biologically active fungal endophyte from the genus Penicillium, isolated from a squill (Urginea maritima) leaf, was further examined. Chloroform‐based extraction of its growth medium was similarly active against phytopathogens. High‐performance liquid chromatography separation followed by gas chromatography/mass spectrometry analysis revealed a single compound—mycophenolic acid—as the main contributor to the biological activity of the organic extract.https://doi.org/10.1111/1751-7915.14203 |
| spellingShingle | Neri Azar Orna Liarzi Maor Zavitan Mohamed Samara Ahmed Nasser David Ezra Endophytic Penicillium species secretes mycophenolic acid that inhibits the growth of phytopathogenic fungi Microbial Biotechnology |
| title | Endophytic Penicillium species secretes mycophenolic acid that inhibits the growth of phytopathogenic fungi |
| title_full | Endophytic Penicillium species secretes mycophenolic acid that inhibits the growth of phytopathogenic fungi |
| title_fullStr | Endophytic Penicillium species secretes mycophenolic acid that inhibits the growth of phytopathogenic fungi |
| title_full_unstemmed | Endophytic Penicillium species secretes mycophenolic acid that inhibits the growth of phytopathogenic fungi |
| title_short | Endophytic Penicillium species secretes mycophenolic acid that inhibits the growth of phytopathogenic fungi |
| title_sort | endophytic penicillium species secretes mycophenolic acid that inhibits the growth of phytopathogenic fungi |
| url | https://doi.org/10.1111/1751-7915.14203 |
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