Jasmonate and Ethylene-Regulated Ethylene Response Factor 22 Promotes Lanolin-Induced Anthocyanin Biosynthesis in ‘Zaosu’ Pear (<i>Pyrus bretschneideri</i> Rehd.) Fruit
Anthocyanin contributes to the coloration of pear fruit and enhances plant defenses. Members of the ethylene response factor (ERF) family play vital roles in hormone and stress signaling and are involved in anthocyanin biosynthesis. Here, <i>PbERF22</i> was identified from the lanolin-in...
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MDPI AG
2020-02-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/2/278 |
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| author | Ting Wu Han-Ting Liu Guang-Ping Zhao Jun-Xing Song Xiao-Li Wang Cheng-Quan Yang Rui Zhai Zhi-Gang Wang Feng-Wang Ma Ling-Fei Xu |
| author_facet | Ting Wu Han-Ting Liu Guang-Ping Zhao Jun-Xing Song Xiao-Li Wang Cheng-Quan Yang Rui Zhai Zhi-Gang Wang Feng-Wang Ma Ling-Fei Xu |
| author_sort | Ting Wu |
| collection | DOAJ |
| description | Anthocyanin contributes to the coloration of pear fruit and enhances plant defenses. Members of the ethylene response factor (ERF) family play vital roles in hormone and stress signaling and are involved in anthocyanin biosynthesis. Here, <i>PbERF22</i> was identified from the lanolin-induced red fruit of ‘Zaosu’ pear (<i>Pyrus bretschneideri</i> Rehd.) using a comparative transcriptome analysis. Its expression level was up- and down-regulated by methyl jasmonate and 1-methylcyclopropene plus lanolin treatments, respectively, which indicated that <i>PbERF22</i> responded to the jasmonate- and ethylene-signaling pathways. In addition, transiently overexpressed <i>PbERF22</i> induced anthocyanin biosynthesis in ‘Zaosu’ fruit, and a quantitative PCR analysis further confirmed that <i>PbERF22</i> facilitated the expression of anthocyanin biosynthetic structural and regulatory genes. Moreover, a dual luciferase assay showed that <i>PbERF22</i> enhanced the activation effects of <i>PbMYB10</i> and <i>PbMYB10b</i> on the <i>PbUFGT</i> promoter. Therefore, <i>PbERF22</i> responses to jasmonate and ethylene signals and regulates anthocyanin biosynthesis. This provides a new perspective on the correlation between jasmonate−ethylene crosstalk and anthocyanin biosynthesis. |
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| id | doaj.art-a7afa9ed84904d6282defcb67a81d56c |
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| issn | 2218-273X |
| language | English |
| last_indexed | 2024-04-13T04:23:17Z |
| publishDate | 2020-02-01 |
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| series | Biomolecules |
| spelling | doaj.art-a7afa9ed84904d6282defcb67a81d56c2022-12-22T03:02:39ZengMDPI AGBiomolecules2218-273X2020-02-0110227810.3390/biom10020278biom10020278Jasmonate and Ethylene-Regulated Ethylene Response Factor 22 Promotes Lanolin-Induced Anthocyanin Biosynthesis in ‘Zaosu’ Pear (<i>Pyrus bretschneideri</i> Rehd.) FruitTing Wu0Han-Ting Liu1Guang-Ping Zhao2Jun-Xing Song3Xiao-Li Wang4Cheng-Quan Yang5Rui Zhai6Zhi-Gang Wang7Feng-Wang Ma8Ling-Fei Xu9College of Horticulture, Northwest A&F University, Taicheng Road NO.3, Yangling 712100, Shaanxi Province, ChinaCollege of Horticulture, Northwest A&F University, Taicheng Road NO.3, Yangling 712100, Shaanxi Province, ChinaCollege of Horticulture, Northwest A&F University, Taicheng Road NO.3, Yangling 712100, Shaanxi Province, ChinaCollege of Horticulture, Northwest A&F University, Taicheng Road NO.3, Yangling 712100, Shaanxi Province, ChinaCollege of Horticulture, Northwest A&F University, Taicheng Road NO.3, Yangling 712100, Shaanxi Province, ChinaCollege of Horticulture, Northwest A&F University, Taicheng Road NO.3, Yangling 712100, Shaanxi Province, ChinaCollege of Horticulture, Northwest A&F University, Taicheng Road NO.3, Yangling 712100, Shaanxi Province, ChinaCollege of Horticulture, Northwest A&F University, Taicheng Road NO.3, Yangling 712100, Shaanxi Province, ChinaCollege of Horticulture, Northwest A&F University, Taicheng Road NO.3, Yangling 712100, Shaanxi Province, ChinaCollege of Horticulture, Northwest A&F University, Taicheng Road NO.3, Yangling 712100, Shaanxi Province, ChinaAnthocyanin contributes to the coloration of pear fruit and enhances plant defenses. Members of the ethylene response factor (ERF) family play vital roles in hormone and stress signaling and are involved in anthocyanin biosynthesis. Here, <i>PbERF22</i> was identified from the lanolin-induced red fruit of ‘Zaosu’ pear (<i>Pyrus bretschneideri</i> Rehd.) using a comparative transcriptome analysis. Its expression level was up- and down-regulated by methyl jasmonate and 1-methylcyclopropene plus lanolin treatments, respectively, which indicated that <i>PbERF22</i> responded to the jasmonate- and ethylene-signaling pathways. In addition, transiently overexpressed <i>PbERF22</i> induced anthocyanin biosynthesis in ‘Zaosu’ fruit, and a quantitative PCR analysis further confirmed that <i>PbERF22</i> facilitated the expression of anthocyanin biosynthetic structural and regulatory genes. Moreover, a dual luciferase assay showed that <i>PbERF22</i> enhanced the activation effects of <i>PbMYB10</i> and <i>PbMYB10b</i> on the <i>PbUFGT</i> promoter. Therefore, <i>PbERF22</i> responses to jasmonate and ethylene signals and regulates anthocyanin biosynthesis. This provides a new perspective on the correlation between jasmonate−ethylene crosstalk and anthocyanin biosynthesis.https://www.mdpi.com/2218-273X/10/2/278pearanthocyanin<i>pberf22</i>myb transcription factorsethylenejasmonate |
| spellingShingle | Ting Wu Han-Ting Liu Guang-Ping Zhao Jun-Xing Song Xiao-Li Wang Cheng-Quan Yang Rui Zhai Zhi-Gang Wang Feng-Wang Ma Ling-Fei Xu Jasmonate and Ethylene-Regulated Ethylene Response Factor 22 Promotes Lanolin-Induced Anthocyanin Biosynthesis in ‘Zaosu’ Pear (<i>Pyrus bretschneideri</i> Rehd.) Fruit Biomolecules pear anthocyanin <i>pberf22</i> myb transcription factors ethylene jasmonate |
| title | Jasmonate and Ethylene-Regulated Ethylene Response Factor 22 Promotes Lanolin-Induced Anthocyanin Biosynthesis in ‘Zaosu’ Pear (<i>Pyrus bretschneideri</i> Rehd.) Fruit |
| title_full | Jasmonate and Ethylene-Regulated Ethylene Response Factor 22 Promotes Lanolin-Induced Anthocyanin Biosynthesis in ‘Zaosu’ Pear (<i>Pyrus bretschneideri</i> Rehd.) Fruit |
| title_fullStr | Jasmonate and Ethylene-Regulated Ethylene Response Factor 22 Promotes Lanolin-Induced Anthocyanin Biosynthesis in ‘Zaosu’ Pear (<i>Pyrus bretschneideri</i> Rehd.) Fruit |
| title_full_unstemmed | Jasmonate and Ethylene-Regulated Ethylene Response Factor 22 Promotes Lanolin-Induced Anthocyanin Biosynthesis in ‘Zaosu’ Pear (<i>Pyrus bretschneideri</i> Rehd.) Fruit |
| title_short | Jasmonate and Ethylene-Regulated Ethylene Response Factor 22 Promotes Lanolin-Induced Anthocyanin Biosynthesis in ‘Zaosu’ Pear (<i>Pyrus bretschneideri</i> Rehd.) Fruit |
| title_sort | jasmonate and ethylene regulated ethylene response factor 22 promotes lanolin induced anthocyanin biosynthesis in zaosu pear i pyrus bretschneideri i rehd fruit |
| topic | pear anthocyanin <i>pberf22</i> myb transcription factors ethylene jasmonate |
| url | https://www.mdpi.com/2218-273X/10/2/278 |
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