Comparative study of transcriptome profiles in the eyes and brain of the tiger puffer Takifugu rubripes brooder under red light filtered LED
Light spectrum is an important environmental factor affecting fish activities. However, the existing studies on spectrum are usually conducted under monochromatic light, which are multiple variables compared to the natural light conditions and increase uncertainty. The results may be caused by the a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423001771 |
| _version_ | 1797752997967560704 |
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| author | Guoyu Wang Zhihao Wu Lijuan Wang Yuxia Zou Yuyu Xiong Feng You |
| author_facet | Guoyu Wang Zhihao Wu Lijuan Wang Yuxia Zou Yuyu Xiong Feng You |
| author_sort | Guoyu Wang |
| collection | DOAJ |
| description | Light spectrum is an important environmental factor affecting fish activities. However, the existing studies on spectrum are usually conducted under monochromatic light, which are multiple variables compared to the natural light conditions and increase uncertainty. The results may be caused by the absence of all other chromatic lights. The tiger puffer Takifugu rubripes is an important commercial marine fish in China, Japan, and Korea. In this study, the effects of red light on the eyes and brain of T. rubripes brooder, including histological changes in retina and optic tectum, the levels of oxidative stress indicators in serum, and changes in gene expression at transcriptome level were examined, after the treatment of full spectrum LED (Fs) and red light filtered LED (Rf) for 52 days. The results showed that the growth and histological structure of retina and optic tectum were not significantly altered. While, Rf significantly decreased the activity of superoxide dismutase and catalase, and increased the content of cortisol (P < 0.05), but had no effect on the levels of glutathione peroxidase and malondialdehyde. Transcriptome data revealed that there were 147 differentially expressed genes (DEGs) in the eyes of Rf vs Fs. Many DEGs related to vision changed significantly. KEGG enrichment and GSEA analyses showed that the significantly enriched arachidonic acid pathway down-regulated in the Rf group, in which genes related to sexual maturation and ovulation presented significant changes, suggesting that Rf may affect the ovulation process through this pathway. In the brain, the number of DEGs was only 20, which are most correlated with circadian rhythm. The present study displayed for the first time the role of red light in promoting ovulation in mature fish by filtering it, which may provide more supports to highlight the function of red light in fish culture. |
| first_indexed | 2024-03-12T17:11:31Z |
| format | Article |
| id | doaj.art-a7b17a283c104a56abb0cd0ca07bf975 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-12T17:11:31Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-a7b17a283c104a56abb0cd0ca07bf9752023-08-06T04:37:34ZengElsevierAquaculture Reports2352-51342023-08-0131101638Comparative study of transcriptome profiles in the eyes and brain of the tiger puffer Takifugu rubripes brooder under red light filtered LEDGuoyu Wang0Zhihao Wu1Lijuan Wang2Yuxia Zou3Yuyu Xiong4Feng You5Qingdao Agricultural University, Qingdao 266237, China; CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China; Corresponding authors at: CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, ChinaDalian Ocean University, Dalian 116023, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China; Corresponding authors at: CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.Light spectrum is an important environmental factor affecting fish activities. However, the existing studies on spectrum are usually conducted under monochromatic light, which are multiple variables compared to the natural light conditions and increase uncertainty. The results may be caused by the absence of all other chromatic lights. The tiger puffer Takifugu rubripes is an important commercial marine fish in China, Japan, and Korea. In this study, the effects of red light on the eyes and brain of T. rubripes brooder, including histological changes in retina and optic tectum, the levels of oxidative stress indicators in serum, and changes in gene expression at transcriptome level were examined, after the treatment of full spectrum LED (Fs) and red light filtered LED (Rf) for 52 days. The results showed that the growth and histological structure of retina and optic tectum were not significantly altered. While, Rf significantly decreased the activity of superoxide dismutase and catalase, and increased the content of cortisol (P < 0.05), but had no effect on the levels of glutathione peroxidase and malondialdehyde. Transcriptome data revealed that there were 147 differentially expressed genes (DEGs) in the eyes of Rf vs Fs. Many DEGs related to vision changed significantly. KEGG enrichment and GSEA analyses showed that the significantly enriched arachidonic acid pathway down-regulated in the Rf group, in which genes related to sexual maturation and ovulation presented significant changes, suggesting that Rf may affect the ovulation process through this pathway. In the brain, the number of DEGs was only 20, which are most correlated with circadian rhythm. The present study displayed for the first time the role of red light in promoting ovulation in mature fish by filtering it, which may provide more supports to highlight the function of red light in fish culture.http://www.sciencedirect.com/science/article/pii/S2352513423001771PufferRed light filtered LEDEyes and brainOxidative stress indexTranscriptome |
| spellingShingle | Guoyu Wang Zhihao Wu Lijuan Wang Yuxia Zou Yuyu Xiong Feng You Comparative study of transcriptome profiles in the eyes and brain of the tiger puffer Takifugu rubripes brooder under red light filtered LED Aquaculture Reports Puffer Red light filtered LED Eyes and brain Oxidative stress index Transcriptome |
| title | Comparative study of transcriptome profiles in the eyes and brain of the tiger puffer Takifugu rubripes brooder under red light filtered LED |
| title_full | Comparative study of transcriptome profiles in the eyes and brain of the tiger puffer Takifugu rubripes brooder under red light filtered LED |
| title_fullStr | Comparative study of transcriptome profiles in the eyes and brain of the tiger puffer Takifugu rubripes brooder under red light filtered LED |
| title_full_unstemmed | Comparative study of transcriptome profiles in the eyes and brain of the tiger puffer Takifugu rubripes brooder under red light filtered LED |
| title_short | Comparative study of transcriptome profiles in the eyes and brain of the tiger puffer Takifugu rubripes brooder under red light filtered LED |
| title_sort | comparative study of transcriptome profiles in the eyes and brain of the tiger puffer takifugu rubripes brooder under red light filtered led |
| topic | Puffer Red light filtered LED Eyes and brain Oxidative stress index Transcriptome |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423001771 |
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