Unraveling the Diverse Profile of N-Acyl Homoserine Lactone Signals and Their Role in the Regulation of Biofilm Formation in <i>Porphyra haitanensis</i>-Associated <i>Pseudoalteromonas galatheae</i>
N-acyl homoserine lactones (AHLs) are small, diffusible chemical signal molecules that serve as social interaction tools for bacteria, enabling them to synchronize their collective actions in a density-dependent manner through quorum sensing (QS). The QS activity from epiphytic bacteria of the red m...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/9/2228 |
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| author | Muhammad Aslam Pengbing Pei Peilin Ye Tangcheng Li Honghao Liang Zezhi Zhang Xiao Ke Weizhou Chen Hong Du |
| author_facet | Muhammad Aslam Pengbing Pei Peilin Ye Tangcheng Li Honghao Liang Zezhi Zhang Xiao Ke Weizhou Chen Hong Du |
| author_sort | Muhammad Aslam |
| collection | DOAJ |
| description | N-acyl homoserine lactones (AHLs) are small, diffusible chemical signal molecules that serve as social interaction tools for bacteria, enabling them to synchronize their collective actions in a density-dependent manner through quorum sensing (QS). The QS activity from epiphytic bacteria of the red macroalgae <i>Porphyra haitanensis</i>, along with its involvement in biofilm formation and regulation, remains unexplored in prior scientific inquiries. Therefore, this study explores the AHL signal molecules produced by epiphytic bacteria. The bacterium isolated from the surface of <i>P. haitanensis</i> was identified as <i>Pseudoalteromonas galatheae</i> by 16s rRNA gene sequencing and screened for AHLs using two AHL reporter strains, <i>Agrobacterium tumefaciens</i> A136 and <i>Chromobacterium violaceum</i> CV026. The crystal violet assay was used for the biofilm-forming phenotype. The inferences revealed that <i>P. galatheae</i> produces four different types of AHL molecules, i.e., C4-HSL, C8-HSL, C18-HSL, and 3-oxo-C16-HSL, and it was observed that its biofilm formation phenotype is regulated by QS molecules. This is the first study providing insights into the QS activity, diverse AHL profile, and regulatory mechanisms that govern the biofilm formation phenotype of <i>P. galatheae</i>. These findings offer valuable insights for future investigations exploring the role of AHL producing epiphytes and biofilms in the life cycle of <i>P. haitanensis</i>. |
| first_indexed | 2024-03-10T22:27:19Z |
| format | Article |
| id | doaj.art-a7b2679bebc34587ba74eaea0b1b6bd4 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T22:27:19Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a7b2679bebc34587ba74eaea0b1b6bd42023-11-19T12:02:23ZengMDPI AGMicroorganisms2076-26072023-09-01119222810.3390/microorganisms11092228Unraveling the Diverse Profile of N-Acyl Homoserine Lactone Signals and Their Role in the Regulation of Biofilm Formation in <i>Porphyra haitanensis</i>-Associated <i>Pseudoalteromonas galatheae</i>Muhammad Aslam0Pengbing Pei1Peilin Ye2Tangcheng Li3Honghao Liang4Zezhi Zhang5Xiao Ke6Weizhou Chen7Hong Du8Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, College of Science, Shantou University, Shantou 515063, ChinaGuangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, College of Science, Shantou University, Shantou 515063, ChinaGuangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, College of Science, Shantou University, Shantou 515063, ChinaGuangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, College of Science, Shantou University, Shantou 515063, ChinaGuangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, College of Science, Shantou University, Shantou 515063, ChinaGuangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, College of Science, Shantou University, Shantou 515063, ChinaGuangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, College of Science, Shantou University, Shantou 515063, ChinaGuangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, College of Science, Shantou University, Shantou 515063, ChinaGuangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, College of Science, Shantou University, Shantou 515063, ChinaN-acyl homoserine lactones (AHLs) are small, diffusible chemical signal molecules that serve as social interaction tools for bacteria, enabling them to synchronize their collective actions in a density-dependent manner through quorum sensing (QS). The QS activity from epiphytic bacteria of the red macroalgae <i>Porphyra haitanensis</i>, along with its involvement in biofilm formation and regulation, remains unexplored in prior scientific inquiries. Therefore, this study explores the AHL signal molecules produced by epiphytic bacteria. The bacterium isolated from the surface of <i>P. haitanensis</i> was identified as <i>Pseudoalteromonas galatheae</i> by 16s rRNA gene sequencing and screened for AHLs using two AHL reporter strains, <i>Agrobacterium tumefaciens</i> A136 and <i>Chromobacterium violaceum</i> CV026. The crystal violet assay was used for the biofilm-forming phenotype. The inferences revealed that <i>P. galatheae</i> produces four different types of AHL molecules, i.e., C4-HSL, C8-HSL, C18-HSL, and 3-oxo-C16-HSL, and it was observed that its biofilm formation phenotype is regulated by QS molecules. This is the first study providing insights into the QS activity, diverse AHL profile, and regulatory mechanisms that govern the biofilm formation phenotype of <i>P. galatheae</i>. These findings offer valuable insights for future investigations exploring the role of AHL producing epiphytes and biofilms in the life cycle of <i>P. haitanensis</i>.https://www.mdpi.com/2076-2607/11/9/2228acyl homoserine lactonesbiofilmsmacroalgae<i>Pseudoalteromonas galatheae</i><i>Porphyra haitanensis</i>quorum sensing |
| spellingShingle | Muhammad Aslam Pengbing Pei Peilin Ye Tangcheng Li Honghao Liang Zezhi Zhang Xiao Ke Weizhou Chen Hong Du Unraveling the Diverse Profile of N-Acyl Homoserine Lactone Signals and Their Role in the Regulation of Biofilm Formation in <i>Porphyra haitanensis</i>-Associated <i>Pseudoalteromonas galatheae</i> Microorganisms acyl homoserine lactones biofilms macroalgae <i>Pseudoalteromonas galatheae</i> <i>Porphyra haitanensis</i> quorum sensing |
| title | Unraveling the Diverse Profile of N-Acyl Homoserine Lactone Signals and Their Role in the Regulation of Biofilm Formation in <i>Porphyra haitanensis</i>-Associated <i>Pseudoalteromonas galatheae</i> |
| title_full | Unraveling the Diverse Profile of N-Acyl Homoserine Lactone Signals and Their Role in the Regulation of Biofilm Formation in <i>Porphyra haitanensis</i>-Associated <i>Pseudoalteromonas galatheae</i> |
| title_fullStr | Unraveling the Diverse Profile of N-Acyl Homoserine Lactone Signals and Their Role in the Regulation of Biofilm Formation in <i>Porphyra haitanensis</i>-Associated <i>Pseudoalteromonas galatheae</i> |
| title_full_unstemmed | Unraveling the Diverse Profile of N-Acyl Homoserine Lactone Signals and Their Role in the Regulation of Biofilm Formation in <i>Porphyra haitanensis</i>-Associated <i>Pseudoalteromonas galatheae</i> |
| title_short | Unraveling the Diverse Profile of N-Acyl Homoserine Lactone Signals and Their Role in the Regulation of Biofilm Formation in <i>Porphyra haitanensis</i>-Associated <i>Pseudoalteromonas galatheae</i> |
| title_sort | unraveling the diverse profile of n acyl homoserine lactone signals and their role in the regulation of biofilm formation in i porphyra haitanensis i associated i pseudoalteromonas galatheae i |
| topic | acyl homoserine lactones biofilms macroalgae <i>Pseudoalteromonas galatheae</i> <i>Porphyra haitanensis</i> quorum sensing |
| url | https://www.mdpi.com/2076-2607/11/9/2228 |
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