Dynamic response of the cell traction force to osmotic shock
Abstract Osmotic pressure is vital to many physiological activities, such as cell proliferation, wound healing and disease treatment. However, how cells interact with the extracellular matrix (ECM) when subjected to osmotic shock remains unclear. Here, we visualize the mechanical interactions betwee...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2023-10-01
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| Series: | Microsystems & Nanoengineering |
| Online Access: | https://doi.org/10.1038/s41378-023-00603-2 |
| _version_ | 1797559039510446080 |
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| author | Yongman Liu Wenjie Wu Shuo Feng Ye Chen Xiaoping Wu Qingchuan Zhang Shangquan Wu |
| author_facet | Yongman Liu Wenjie Wu Shuo Feng Ye Chen Xiaoping Wu Qingchuan Zhang Shangquan Wu |
| author_sort | Yongman Liu |
| collection | DOAJ |
| description | Abstract Osmotic pressure is vital to many physiological activities, such as cell proliferation, wound healing and disease treatment. However, how cells interact with the extracellular matrix (ECM) when subjected to osmotic shock remains unclear. Here, we visualize the mechanical interactions between cells and the ECM during osmotic shock by quantifying the dynamic evolution of the cell traction force. We show that both hypertonic and hypotonic shocks induce continuous and large changes in cell traction force. Moreover, the traction force varies with cell volume: the traction force increases as cells shrink and decreases as cells swell. However, the direction of the traction force is independent of cell volume changes and is always toward the center of the cell-substrate interface. Furthermore, we reveal a mechanical mechanism in which the change in cortical tension caused by osmotic shock leads to the variation in traction force, which suggests a simple method for measuring changes in cell cortical tension. These findings provide new insights into the mechanical force response of cells to the external environment and may provide a deeper understanding of how the ECM regulates cell structure and function. Traction force exerted by cells under hypertonic and hypotonic shocks. Scale bar, 200 Pa. Color bar, Pa. The black arrows represent the tangential traction forces. |
| first_indexed | 2024-03-10T17:40:05Z |
| format | Article |
| id | doaj.art-a7b4acbaaf5b40ecb1745c03f2cea45f |
| institution | Directory Open Access Journal |
| issn | 2055-7434 |
| language | English |
| last_indexed | 2024-03-10T17:40:05Z |
| publishDate | 2023-10-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Microsystems & Nanoengineering |
| spelling | doaj.art-a7b4acbaaf5b40ecb1745c03f2cea45f2023-11-20T09:44:39ZengNature Publishing GroupMicrosystems & Nanoengineering2055-74342023-10-019111110.1038/s41378-023-00603-2Dynamic response of the cell traction force to osmotic shockYongman Liu0Wenjie Wu1Shuo Feng2Ye Chen3Xiaoping Wu4Qingchuan Zhang5Shangquan Wu6School of Biomedical Engineering, Anhui Medical UniversityCAS Key Laboratory of Mechanical Behavior and Design of Material, Department of Modern Mechanics, CAS Center for Excellence in Complex System Mechanics, University of Science and Technology of ChinaCAS Key Laboratory of Mechanical Behavior and Design of Material, Department of Modern Mechanics, CAS Center for Excellence in Complex System Mechanics, University of Science and Technology of ChinaCAS Key Laboratory of Mechanical Behavior and Design of Material, Department of Modern Mechanics, CAS Center for Excellence in Complex System Mechanics, University of Science and Technology of ChinaCAS Key Laboratory of Mechanical Behavior and Design of Material, Department of Modern Mechanics, CAS Center for Excellence in Complex System Mechanics, University of Science and Technology of ChinaCAS Key Laboratory of Mechanical Behavior and Design of Material, Department of Modern Mechanics, CAS Center for Excellence in Complex System Mechanics, University of Science and Technology of ChinaCAS Key Laboratory of Mechanical Behavior and Design of Material, Department of Modern Mechanics, CAS Center for Excellence in Complex System Mechanics, University of Science and Technology of ChinaAbstract Osmotic pressure is vital to many physiological activities, such as cell proliferation, wound healing and disease treatment. However, how cells interact with the extracellular matrix (ECM) when subjected to osmotic shock remains unclear. Here, we visualize the mechanical interactions between cells and the ECM during osmotic shock by quantifying the dynamic evolution of the cell traction force. We show that both hypertonic and hypotonic shocks induce continuous and large changes in cell traction force. Moreover, the traction force varies with cell volume: the traction force increases as cells shrink and decreases as cells swell. However, the direction of the traction force is independent of cell volume changes and is always toward the center of the cell-substrate interface. Furthermore, we reveal a mechanical mechanism in which the change in cortical tension caused by osmotic shock leads to the variation in traction force, which suggests a simple method for measuring changes in cell cortical tension. These findings provide new insights into the mechanical force response of cells to the external environment and may provide a deeper understanding of how the ECM regulates cell structure and function. Traction force exerted by cells under hypertonic and hypotonic shocks. Scale bar, 200 Pa. Color bar, Pa. The black arrows represent the tangential traction forces.https://doi.org/10.1038/s41378-023-00603-2 |
| spellingShingle | Yongman Liu Wenjie Wu Shuo Feng Ye Chen Xiaoping Wu Qingchuan Zhang Shangquan Wu Dynamic response of the cell traction force to osmotic shock Microsystems & Nanoengineering |
| title | Dynamic response of the cell traction force to osmotic shock |
| title_full | Dynamic response of the cell traction force to osmotic shock |
| title_fullStr | Dynamic response of the cell traction force to osmotic shock |
| title_full_unstemmed | Dynamic response of the cell traction force to osmotic shock |
| title_short | Dynamic response of the cell traction force to osmotic shock |
| title_sort | dynamic response of the cell traction force to osmotic shock |
| url | https://doi.org/10.1038/s41378-023-00603-2 |
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