Transcriptome analysis of Corvus splendens reveals a repertoire of antimicrobial peptides
Abstract Multidrug resistance has become a global health problem associated with high morbidity and mortality. Antimicrobial peptides have been acknowledged as potential leads for prospective anti-infectives. Owing to their scavenging lifestyle, Corvus splendens is thought to have developed robust i...
| Autors principals: | , , , , , , , , |
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| Format: | Article |
| Idioma: | English |
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Nature Portfolio
2023-10-01
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| Col·lecció: | Scientific Reports |
| Accés en línia: | https://doi.org/10.1038/s41598-023-45875-w |
| _version_ | 1827770677803876352 |
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| author | Shalini Kannoth Nemat Ali Ganesh K. Prasanth Kumar Arvind Mohamed Mohany Preety Sweta Hembrom Shemmy Sadanandan Deepa Azhchath Vasu Tony Grace |
| author_facet | Shalini Kannoth Nemat Ali Ganesh K. Prasanth Kumar Arvind Mohamed Mohany Preety Sweta Hembrom Shemmy Sadanandan Deepa Azhchath Vasu Tony Grace |
| author_sort | Shalini Kannoth |
| collection | DOAJ |
| description | Abstract Multidrug resistance has become a global health problem associated with high morbidity and mortality. Antimicrobial peptides have been acknowledged as potential leads for prospective anti-infectives. Owing to their scavenging lifestyle, Corvus splendens is thought to have developed robust immunity to pathogens found in their diet, implying that they have evolved mechanisms to resist infection. In the current study, the transcriptome of C. splendens was sequenced, and de novo assembled to identify the presence of antimicrobial peptide genes. 72.09 million high-quality clean reads were obtained which were then de novo assembled into 3,43,503 transcripts and 74,958 unigenes. About 37,559 unigenes were successfully annotated using SwissProt, Pfam, GO, and KEGG databases. A search against APD3, CAMPR3 and LAMP databases identified 63 AMP candidates belonging to more than 20 diverse families and functional classes. mRNA of AvBD-2, AvBD-13 and CATH-2 were found to be differentially expressed between the three tested crows as well as among the tissues. We also characterized Corvus Cathelicidin 2 (CATH-2) to gain knowledge of its antimicrobial mechanisms. The CD spectroscopy of synthesized mature Corvus CATH-2 peptide displayed an amphipathic α-helical structure. Though the synthetic CATH-2 caused hemolysis of human RBC, it also exhibited antimicrobial activity against E. coli, S. aureus, and B. cereus. Docking simulation results revealed that this peptide could bind to the LPS binding site of MD-2, which may prevent LPS from entering the MD-2 binding pocket, and trigger TLR4 signaling pathway. The Corvus CATH-2 characterized in this study could aid in the development of novel therapeutics. |
| first_indexed | 2024-03-11T12:41:59Z |
| format | Article |
| id | doaj.art-a7b51e34b45b4b1b8c4180faee4f55f0 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-11T12:41:59Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a7b51e34b45b4b1b8c4180faee4f55f02023-11-05T12:14:13ZengNature PortfolioScientific Reports2045-23222023-10-0113112210.1038/s41598-023-45875-wTranscriptome analysis of Corvus splendens reveals a repertoire of antimicrobial peptidesShalini Kannoth0Nemat Ali1Ganesh K. Prasanth2Kumar Arvind3Mohamed Mohany4Preety Sweta Hembrom5Shemmy Sadanandan6Deepa Azhchath Vasu7Tony Grace8Department of Genomic Science, School of Biological Sciences, Central University of KeralaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud UniversityDepartment of Biochemistry and Molecular Biology, School of Biological Sciences, Central University of KeralaNeurogenetics Branch, National Institute of Neurological Disorder and Stroke, National Institute of HealthDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud UniversityDepartment of Genomic Science, School of Biological Sciences, Central University of KeralaDepartment of Genomic Science, School of Biological Sciences, Central University of KeralaDepartment of Genomic Science, School of Biological Sciences, Central University of KeralaDepartment of Genomic Science, School of Biological Sciences, Central University of KeralaAbstract Multidrug resistance has become a global health problem associated with high morbidity and mortality. Antimicrobial peptides have been acknowledged as potential leads for prospective anti-infectives. Owing to their scavenging lifestyle, Corvus splendens is thought to have developed robust immunity to pathogens found in their diet, implying that they have evolved mechanisms to resist infection. In the current study, the transcriptome of C. splendens was sequenced, and de novo assembled to identify the presence of antimicrobial peptide genes. 72.09 million high-quality clean reads were obtained which were then de novo assembled into 3,43,503 transcripts and 74,958 unigenes. About 37,559 unigenes were successfully annotated using SwissProt, Pfam, GO, and KEGG databases. A search against APD3, CAMPR3 and LAMP databases identified 63 AMP candidates belonging to more than 20 diverse families and functional classes. mRNA of AvBD-2, AvBD-13 and CATH-2 were found to be differentially expressed between the three tested crows as well as among the tissues. We also characterized Corvus Cathelicidin 2 (CATH-2) to gain knowledge of its antimicrobial mechanisms. The CD spectroscopy of synthesized mature Corvus CATH-2 peptide displayed an amphipathic α-helical structure. Though the synthetic CATH-2 caused hemolysis of human RBC, it also exhibited antimicrobial activity against E. coli, S. aureus, and B. cereus. Docking simulation results revealed that this peptide could bind to the LPS binding site of MD-2, which may prevent LPS from entering the MD-2 binding pocket, and trigger TLR4 signaling pathway. The Corvus CATH-2 characterized in this study could aid in the development of novel therapeutics.https://doi.org/10.1038/s41598-023-45875-w |
| spellingShingle | Shalini Kannoth Nemat Ali Ganesh K. Prasanth Kumar Arvind Mohamed Mohany Preety Sweta Hembrom Shemmy Sadanandan Deepa Azhchath Vasu Tony Grace Transcriptome analysis of Corvus splendens reveals a repertoire of antimicrobial peptides Scientific Reports |
| title | Transcriptome analysis of Corvus splendens reveals a repertoire of antimicrobial peptides |
| title_full | Transcriptome analysis of Corvus splendens reveals a repertoire of antimicrobial peptides |
| title_fullStr | Transcriptome analysis of Corvus splendens reveals a repertoire of antimicrobial peptides |
| title_full_unstemmed | Transcriptome analysis of Corvus splendens reveals a repertoire of antimicrobial peptides |
| title_short | Transcriptome analysis of Corvus splendens reveals a repertoire of antimicrobial peptides |
| title_sort | transcriptome analysis of corvus splendens reveals a repertoire of antimicrobial peptides |
| url | https://doi.org/10.1038/s41598-023-45875-w |
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