Assessment of simplified ratio-based approaches for quantification of PET [11C]PBR28 data
Abstract Purpose Kinetic modelling with metabolite-corrected arterial plasma is considered the gold standard for quantification of [11C]PBR28 binding to the translocator protein (TSPO), since there is no brain region devoid of TSPO that can serve as reference. The high variability in binding observe...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-07-01
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| Series: | EJNMMI Research |
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| Online Access: | http://link.springer.com/article/10.1186/s13550-017-0304-1 |
| _version_ | 1819154411809669120 |
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| author | Granville J. Matheson Pontus Plavén-Sigray Anton Forsberg Andrea Varrone Lars Farde Simon Cervenka |
| author_facet | Granville J. Matheson Pontus Plavén-Sigray Anton Forsberg Andrea Varrone Lars Farde Simon Cervenka |
| author_sort | Granville J. Matheson |
| collection | DOAJ |
| description | Abstract Purpose Kinetic modelling with metabolite-corrected arterial plasma is considered the gold standard for quantification of [11C]PBR28 binding to the translocator protein (TSPO), since there is no brain region devoid of TSPO that can serve as reference. The high variability in binding observed using this method has motivated the use of simplified ratio-based approaches such as standardised uptake value ratios (SUVRs) and distribution volume (VT) ratios (DVRs); however, the reliability of these measures and their relationship to VT have not been sufficiently evaluated. Methods Data from a previously published [11C]PBR28 test-retest study in 12 healthy subjects were reanalysed. VT was estimated using a two-tissue compartment model. SUVR and DVR values for the frontal cortex were calculated using the whole brain and cerebellum as denominators. Test-retest reliability was assessed for all measures. Interregional correlations were performed for SUV and VT, and principal component analysis (PCA) was applied. Lastly, correlations between ratio-based outcomes and VT were assessed. Results Reliability was high for VT, moderate to high for SUV and SUVR, and poor for DVR. Very high interregional correlations were observed for both VT and SUV (all R 2 > 85%). The PCA showed that almost all variance (>98%) was explained by a single component. Ratio-based methods correlated poorly with VT (all R 2 < 34%, divided by genotype). Conclusions The reliability was good for SUVR, but poor for DVR. Both outcomes showed little to no association with VT, questioning their validity. The high interregional correlations for VT and SUV suggest that after dividing by a denominator region, most of the biologically relevant signal is lost. These observations imply that results from TSPO PET studies using SUVR or DVR estimates should be interpreted with caution. |
| first_indexed | 2024-12-22T15:20:40Z |
| format | Article |
| id | doaj.art-a7b5dc3081944cd7b695a346724dd527 |
| institution | Directory Open Access Journal |
| issn | 2191-219X |
| language | English |
| last_indexed | 2024-12-22T15:20:40Z |
| publishDate | 2017-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | EJNMMI Research |
| spelling | doaj.art-a7b5dc3081944cd7b695a346724dd5272022-12-21T18:21:39ZengSpringerOpenEJNMMI Research2191-219X2017-07-01711610.1186/s13550-017-0304-1Assessment of simplified ratio-based approaches for quantification of PET [11C]PBR28 dataGranville J. Matheson0Pontus Plavén-Sigray1Anton Forsberg2Andrea Varrone3Lars Farde4Simon Cervenka5Department of Clinical Neuroscience, Centre for Psychiatry Research, Karolinska Institutet and Stockholm Health Care Services, Stockholm County CouncilDepartment of Clinical Neuroscience, Centre for Psychiatry Research, Karolinska Institutet and Stockholm Health Care Services, Stockholm County CouncilDepartment of Clinical Neuroscience, Centre for Psychiatry Research, Karolinska Institutet and Stockholm Health Care Services, Stockholm County CouncilDepartment of Clinical Neuroscience, Centre for Psychiatry Research, Karolinska Institutet and Stockholm Health Care Services, Stockholm County CouncilDepartment of Clinical Neuroscience, Centre for Psychiatry Research, Karolinska Institutet and Stockholm Health Care Services, Stockholm County CouncilDepartment of Clinical Neuroscience, Centre for Psychiatry Research, Karolinska Institutet and Stockholm Health Care Services, Stockholm County CouncilAbstract Purpose Kinetic modelling with metabolite-corrected arterial plasma is considered the gold standard for quantification of [11C]PBR28 binding to the translocator protein (TSPO), since there is no brain region devoid of TSPO that can serve as reference. The high variability in binding observed using this method has motivated the use of simplified ratio-based approaches such as standardised uptake value ratios (SUVRs) and distribution volume (VT) ratios (DVRs); however, the reliability of these measures and their relationship to VT have not been sufficiently evaluated. Methods Data from a previously published [11C]PBR28 test-retest study in 12 healthy subjects were reanalysed. VT was estimated using a two-tissue compartment model. SUVR and DVR values for the frontal cortex were calculated using the whole brain and cerebellum as denominators. Test-retest reliability was assessed for all measures. Interregional correlations were performed for SUV and VT, and principal component analysis (PCA) was applied. Lastly, correlations between ratio-based outcomes and VT were assessed. Results Reliability was high for VT, moderate to high for SUV and SUVR, and poor for DVR. Very high interregional correlations were observed for both VT and SUV (all R 2 > 85%). The PCA showed that almost all variance (>98%) was explained by a single component. Ratio-based methods correlated poorly with VT (all R 2 < 34%, divided by genotype). Conclusions The reliability was good for SUVR, but poor for DVR. Both outcomes showed little to no association with VT, questioning their validity. The high interregional correlations for VT and SUV suggest that after dividing by a denominator region, most of the biologically relevant signal is lost. These observations imply that results from TSPO PET studies using SUVR or DVR estimates should be interpreted with caution.http://link.springer.com/article/10.1186/s13550-017-0304-1PETTSPO[11C]PBR28SUVRDVR |
| spellingShingle | Granville J. Matheson Pontus Plavén-Sigray Anton Forsberg Andrea Varrone Lars Farde Simon Cervenka Assessment of simplified ratio-based approaches for quantification of PET [11C]PBR28 data EJNMMI Research PET TSPO [11C]PBR28 SUVR DVR |
| title | Assessment of simplified ratio-based approaches for quantification of PET [11C]PBR28 data |
| title_full | Assessment of simplified ratio-based approaches for quantification of PET [11C]PBR28 data |
| title_fullStr | Assessment of simplified ratio-based approaches for quantification of PET [11C]PBR28 data |
| title_full_unstemmed | Assessment of simplified ratio-based approaches for quantification of PET [11C]PBR28 data |
| title_short | Assessment of simplified ratio-based approaches for quantification of PET [11C]PBR28 data |
| title_sort | assessment of simplified ratio based approaches for quantification of pet 11c pbr28 data |
| topic | PET TSPO [11C]PBR28 SUVR DVR |
| url | http://link.springer.com/article/10.1186/s13550-017-0304-1 |
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