Changes in Carotenoid Concentration and Expression of Carotenoid Biosynthesis Genes in <i>Daucus carota</i> Taproots in Response to Increased Salinity
Studying the changes of carotenoids in the taproot of carrots under salt treatment is helpful to probe the salt stress response mechanism of carrots. The carotenoid concentration and the expression profiles of 10 carotenoid-related genes were determined in two carrot cultivars with different taproot...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/7/650 |
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| author | Yu-Han Zhao Yuan-Jie Deng Yuan-Hua Wang Ying-Rui Lou Ling-Feng He Hui Liu Tong Li Zhi-Ming Yan Jing Zhuang Ai-Sheng Xiong |
| author_facet | Yu-Han Zhao Yuan-Jie Deng Yuan-Hua Wang Ying-Rui Lou Ling-Feng He Hui Liu Tong Li Zhi-Ming Yan Jing Zhuang Ai-Sheng Xiong |
| author_sort | Yu-Han Zhao |
| collection | DOAJ |
| description | Studying the changes of carotenoids in the taproot of carrots under salt treatment is helpful to probe the salt stress response mechanism of carrots. The carotenoid concentration and the expression profiles of 10 carotenoid-related genes were determined in two carrot cultivars with different taproot colors. Under salt stress, the biosynthesis of carotenoids in the taproot of both ‘KRD’ and ‘BHJS’ was activated. RT-qPCR manifested that the expression levels of <i>DcPSY1</i>, <i>DcPSY2</i>, <i>DcZDS1</i>, <i>DcCRT1</i> and <i>DcCRT2</i> increased significantly in both ‘KRD’ and BHJS’ under salt stress, but <i>DcCHXE</i> transcripts decreased and <i>DcPDS</i> transcripts maintained a basal level compared to that of the control. In the taproot of ‘KRD’, the expression level of <i>DcLCYB</i>, <i>DcLCYE</i> and <i>DcCHXB1</i> climbed dramatically. However, there was no significant change in the taproot of ‘BHJS’. The study showed that salt stress can stimulate the biosynthesis of carotenoids. The accumulation of lutein in the taproots of ‘KRD’ and ‘BHJS’ may be mainly attributed to the variation in <i>DcLCYE</i> and <i>DcCHXB1</i> transcripts. The increase in β-carotene accumulation is speculated to increase salt tolerance. |
| first_indexed | 2024-03-09T13:45:26Z |
| format | Article |
| id | doaj.art-a7ba88c21a74432e9558c2a28d951508 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T13:45:26Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-a7ba88c21a74432e9558c2a28d9515082023-11-30T21:01:41ZengMDPI AGHorticulturae2311-75242022-07-018765010.3390/horticulturae8070650Changes in Carotenoid Concentration and Expression of Carotenoid Biosynthesis Genes in <i>Daucus carota</i> Taproots in Response to Increased SalinityYu-Han Zhao0Yuan-Jie Deng1Yuan-Hua Wang2Ying-Rui Lou3Ling-Feng He4Hui Liu5Tong Li6Zhi-Ming Yan7Jing Zhuang8Ai-Sheng Xiong9State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Modern Horticultural Engineering Technology Center, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212400, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Modern Horticultural Engineering Technology Center, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212400, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaStudying the changes of carotenoids in the taproot of carrots under salt treatment is helpful to probe the salt stress response mechanism of carrots. The carotenoid concentration and the expression profiles of 10 carotenoid-related genes were determined in two carrot cultivars with different taproot colors. Under salt stress, the biosynthesis of carotenoids in the taproot of both ‘KRD’ and ‘BHJS’ was activated. RT-qPCR manifested that the expression levels of <i>DcPSY1</i>, <i>DcPSY2</i>, <i>DcZDS1</i>, <i>DcCRT1</i> and <i>DcCRT2</i> increased significantly in both ‘KRD’ and BHJS’ under salt stress, but <i>DcCHXE</i> transcripts decreased and <i>DcPDS</i> transcripts maintained a basal level compared to that of the control. In the taproot of ‘KRD’, the expression level of <i>DcLCYB</i>, <i>DcLCYE</i> and <i>DcCHXB1</i> climbed dramatically. However, there was no significant change in the taproot of ‘BHJS’. The study showed that salt stress can stimulate the biosynthesis of carotenoids. The accumulation of lutein in the taproots of ‘KRD’ and ‘BHJS’ may be mainly attributed to the variation in <i>DcLCYE</i> and <i>DcCHXB1</i> transcripts. The increase in β-carotene accumulation is speculated to increase salt tolerance.https://www.mdpi.com/2311-7524/8/7/650carotenoidssalt stress<i>DcPSY</i><i>DcLCYE</i>β-carotenecarrot |
| spellingShingle | Yu-Han Zhao Yuan-Jie Deng Yuan-Hua Wang Ying-Rui Lou Ling-Feng He Hui Liu Tong Li Zhi-Ming Yan Jing Zhuang Ai-Sheng Xiong Changes in Carotenoid Concentration and Expression of Carotenoid Biosynthesis Genes in <i>Daucus carota</i> Taproots in Response to Increased Salinity Horticulturae carotenoids salt stress <i>DcPSY</i> <i>DcLCYE</i> β-carotene carrot |
| title | Changes in Carotenoid Concentration and Expression of Carotenoid Biosynthesis Genes in <i>Daucus carota</i> Taproots in Response to Increased Salinity |
| title_full | Changes in Carotenoid Concentration and Expression of Carotenoid Biosynthesis Genes in <i>Daucus carota</i> Taproots in Response to Increased Salinity |
| title_fullStr | Changes in Carotenoid Concentration and Expression of Carotenoid Biosynthesis Genes in <i>Daucus carota</i> Taproots in Response to Increased Salinity |
| title_full_unstemmed | Changes in Carotenoid Concentration and Expression of Carotenoid Biosynthesis Genes in <i>Daucus carota</i> Taproots in Response to Increased Salinity |
| title_short | Changes in Carotenoid Concentration and Expression of Carotenoid Biosynthesis Genes in <i>Daucus carota</i> Taproots in Response to Increased Salinity |
| title_sort | changes in carotenoid concentration and expression of carotenoid biosynthesis genes in i daucus carota i taproots in response to increased salinity |
| topic | carotenoids salt stress <i>DcPSY</i> <i>DcLCYE</i> β-carotene carrot |
| url | https://www.mdpi.com/2311-7524/8/7/650 |
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