Laser Bioprinting of Cells Using UV and Visible Wavelengths: A Comparative DNA Damage Study
Laser-based techniques for printing cells onto different substrates with high precision and resolution present unique opportunities for contributing to a wide range of biomedical applications, including tissue engineering. In this study, laser-induced forward transfer (LIFT) printing was employed to...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Bioengineering |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-5354/9/8/378 |
| _version_ | 1797432446557356032 |
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| author | Panagiotis Karakaidos Christina Kryou Nikiana Simigdala Apostolos Klinakis Ioanna Zergioti |
| author_facet | Panagiotis Karakaidos Christina Kryou Nikiana Simigdala Apostolos Klinakis Ioanna Zergioti |
| author_sort | Panagiotis Karakaidos |
| collection | DOAJ |
| description | Laser-based techniques for printing cells onto different substrates with high precision and resolution present unique opportunities for contributing to a wide range of biomedical applications, including tissue engineering. In this study, laser-induced forward transfer (LIFT) printing was employed to rapidly and accurately deposit patterns of cancer cells in a non-contact manner, using two different wavelengths, 532 and 355 nm. To evaluate the effect of LIFT on the printed cells, their growth and DNA damage profiles were assessed and evaluated quantitatively over several days. The damaging effect of LIFT-printing was thoroughly investigated, for the first time at a single cell level, by counting individual double strand breaks (DSB). Overall, we found that LIFT was able to safely print patterns of breast cancer cells with high viability with little or no heat or shear damage to the cells, as indicated by unperturbed growth and negligible gross DNA damage. |
| first_indexed | 2024-03-09T10:01:46Z |
| format | Article |
| id | doaj.art-a7bb31ef7ff84b2abf2e2900e5be6aaf |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T10:01:46Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-a7bb31ef7ff84b2abf2e2900e5be6aaf2023-12-01T23:25:12ZengMDPI AGBioengineering2306-53542022-08-019837810.3390/bioengineering9080378Laser Bioprinting of Cells Using UV and Visible Wavelengths: A Comparative DNA Damage StudyPanagiotis Karakaidos0Christina Kryou1Nikiana Simigdala2Apostolos Klinakis3Ioanna Zergioti4Biomedical Research Foundation, Academy of Athens, 11527 Athens, GreeceSchool of Applied Mathematical and Physical Sciences, National Technical University of Athens, 15780 Zografou, GreeceBiomedical Research Foundation, Academy of Athens, 11527 Athens, GreeceBiomedical Research Foundation, Academy of Athens, 11527 Athens, GreeceSchool of Applied Mathematical and Physical Sciences, National Technical University of Athens, 15780 Zografou, GreeceLaser-based techniques for printing cells onto different substrates with high precision and resolution present unique opportunities for contributing to a wide range of biomedical applications, including tissue engineering. In this study, laser-induced forward transfer (LIFT) printing was employed to rapidly and accurately deposit patterns of cancer cells in a non-contact manner, using two different wavelengths, 532 and 355 nm. To evaluate the effect of LIFT on the printed cells, their growth and DNA damage profiles were assessed and evaluated quantitatively over several days. The damaging effect of LIFT-printing was thoroughly investigated, for the first time at a single cell level, by counting individual double strand breaks (DSB). Overall, we found that LIFT was able to safely print patterns of breast cancer cells with high viability with little or no heat or shear damage to the cells, as indicated by unperturbed growth and negligible gross DNA damage.https://www.mdpi.com/2306-5354/9/8/378laser-induced forward transfer (LIFT)wavelengthlaser fluenceDNA damagedouble strand breaks |
| spellingShingle | Panagiotis Karakaidos Christina Kryou Nikiana Simigdala Apostolos Klinakis Ioanna Zergioti Laser Bioprinting of Cells Using UV and Visible Wavelengths: A Comparative DNA Damage Study Bioengineering laser-induced forward transfer (LIFT) wavelength laser fluence DNA damage double strand breaks |
| title | Laser Bioprinting of Cells Using UV and Visible Wavelengths: A Comparative DNA Damage Study |
| title_full | Laser Bioprinting of Cells Using UV and Visible Wavelengths: A Comparative DNA Damage Study |
| title_fullStr | Laser Bioprinting of Cells Using UV and Visible Wavelengths: A Comparative DNA Damage Study |
| title_full_unstemmed | Laser Bioprinting of Cells Using UV and Visible Wavelengths: A Comparative DNA Damage Study |
| title_short | Laser Bioprinting of Cells Using UV and Visible Wavelengths: A Comparative DNA Damage Study |
| title_sort | laser bioprinting of cells using uv and visible wavelengths a comparative dna damage study |
| topic | laser-induced forward transfer (LIFT) wavelength laser fluence DNA damage double strand breaks |
| url | https://www.mdpi.com/2306-5354/9/8/378 |
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