Preliminary Interpretations of Epigenetic Profiling of Cord Blood in Preeclampsia
Preeclampsia (PE) is characterized by new-onset hypertension after 20 weeks of pregnancy and results in high maternal and fetal mortality worldwide. It has been reported that PE is associated with abnormalities in the umbilical cord and cord blood. However, previous studies were focused primarily on...
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MDPI AG
2022-05-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/5/888 |
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| author | Junrui Ma Zhongqun Zhan Ning Li Yanli Huang Yan Li Lu Liu Qi Shen Qiao Chu Xiaonan Wang Benqing Wu Hui Zhang |
| author_facet | Junrui Ma Zhongqun Zhan Ning Li Yanli Huang Yan Li Lu Liu Qi Shen Qiao Chu Xiaonan Wang Benqing Wu Hui Zhang |
| author_sort | Junrui Ma |
| collection | DOAJ |
| description | Preeclampsia (PE) is characterized by new-onset hypertension after 20 weeks of pregnancy and results in high maternal and fetal mortality worldwide. It has been reported that PE is associated with abnormalities in the umbilical cord and cord blood. However, previous studies were focused primarily on the transcriptomics level, while the underlying gene regulatory landscapes are still unclear. Thus, we performed the Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq) using the umbilical cord blood samples collected from a patient with superimposed PE and three healthy donors to uncover the chromatin accessibility changes attributed to PE. We have identified genes associated with immunomodulation and hypoxia response that have higher chromatin accessibility close to their transcription start sites. Motif analysis indicated that the <i>GATA</i> family transcription factor binding was enriched in PE and may play an essential regulatory role in the disease progression. Overall, our findings provide an overview of gene regulatory programs and the corresponding downstream pathways associated with PE that may influence the placenta function and fetal growth. |
| first_indexed | 2024-03-10T03:49:31Z |
| format | Article |
| id | doaj.art-a7bb7c4dbb6f411a9e89763be83f6b09 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T03:49:31Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-a7bb7c4dbb6f411a9e89763be83f6b092023-11-23T11:11:05ZengMDPI AGGenes2073-44252022-05-0113588810.3390/genes13050888Preliminary Interpretations of Epigenetic Profiling of Cord Blood in PreeclampsiaJunrui Ma0Zhongqun Zhan1Ning Li2Yanli Huang3Yan Li4Lu Liu5Qi Shen6Qiao Chu7Xiaonan Wang8Benqing Wu9Hui Zhang10School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaInstitute of Translational Medicine, University of Chinese Academy of Sciences Shenzhen Hospital, Shenzhen 518106, ChinaCytotherapy Laboratory, The First Affiliated Hospital (Shenzhen People’s Hospital) Southern University of Science and Technology, Shenzhen 518020, ChinaDepartment of Obstetrics, University of Chinese Academy of Sciences Shenzhen Hospital, Shenzhen 518106, ChinaDepartment of Obstetrics, University of Chinese Academy of Sciences Shenzhen Hospital, Shenzhen 518106, ChinaDepartment of Obstetrics, University of Chinese Academy of Sciences Shenzhen Hospital, Shenzhen 518106, ChinaInstitute of Translational Medicine, University of Chinese Academy of Sciences Shenzhen Hospital, Shenzhen 518106, ChinaSchool of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaSchool of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaInstitute of Translational Medicine, University of Chinese Academy of Sciences Shenzhen Hospital, Shenzhen 518106, ChinaInstitute of Translational Medicine, University of Chinese Academy of Sciences Shenzhen Hospital, Shenzhen 518106, ChinaPreeclampsia (PE) is characterized by new-onset hypertension after 20 weeks of pregnancy and results in high maternal and fetal mortality worldwide. It has been reported that PE is associated with abnormalities in the umbilical cord and cord blood. However, previous studies were focused primarily on the transcriptomics level, while the underlying gene regulatory landscapes are still unclear. Thus, we performed the Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq) using the umbilical cord blood samples collected from a patient with superimposed PE and three healthy donors to uncover the chromatin accessibility changes attributed to PE. We have identified genes associated with immunomodulation and hypoxia response that have higher chromatin accessibility close to their transcription start sites. Motif analysis indicated that the <i>GATA</i> family transcription factor binding was enriched in PE and may play an essential regulatory role in the disease progression. Overall, our findings provide an overview of gene regulatory programs and the corresponding downstream pathways associated with PE that may influence the placenta function and fetal growth.https://www.mdpi.com/2073-4425/13/5/888preeclampsiaepigenetic alternationimmunomodulationhypoxia response<i>GATA</i> family transcription factor |
| spellingShingle | Junrui Ma Zhongqun Zhan Ning Li Yanli Huang Yan Li Lu Liu Qi Shen Qiao Chu Xiaonan Wang Benqing Wu Hui Zhang Preliminary Interpretations of Epigenetic Profiling of Cord Blood in Preeclampsia Genes preeclampsia epigenetic alternation immunomodulation hypoxia response <i>GATA</i> family transcription factor |
| title | Preliminary Interpretations of Epigenetic Profiling of Cord Blood in Preeclampsia |
| title_full | Preliminary Interpretations of Epigenetic Profiling of Cord Blood in Preeclampsia |
| title_fullStr | Preliminary Interpretations of Epigenetic Profiling of Cord Blood in Preeclampsia |
| title_full_unstemmed | Preliminary Interpretations of Epigenetic Profiling of Cord Blood in Preeclampsia |
| title_short | Preliminary Interpretations of Epigenetic Profiling of Cord Blood in Preeclampsia |
| title_sort | preliminary interpretations of epigenetic profiling of cord blood in preeclampsia |
| topic | preeclampsia epigenetic alternation immunomodulation hypoxia response <i>GATA</i> family transcription factor |
| url | https://www.mdpi.com/2073-4425/13/5/888 |
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