Metabolomic Analysis of <i>Cricket paralysis</i> <i>virus</i> Infection in <i>Drosophila</i> S2 Cells Reveals Divergent Effects on Central Carbon Metabolism as Compared with Silkworm Bm5 Cells
High-throughput approaches have opened new opportunities for understanding biological processes such as persistent virus infections, which are widespread. However, the potential of persistent infections to develop towards pathogenesis remains to be investigated, particularly with respect to the role...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/12/4/393 |
| _version_ | 1827719514021691392 |
|---|---|
| author | Luo-Luo Wang Luc Swevers Lieven Van Meulebroek Ivan Meeus Lynn Vanhaecke Guy Smagghe |
| author_facet | Luo-Luo Wang Luc Swevers Lieven Van Meulebroek Ivan Meeus Lynn Vanhaecke Guy Smagghe |
| author_sort | Luo-Luo Wang |
| collection | DOAJ |
| description | High-throughput approaches have opened new opportunities for understanding biological processes such as persistent virus infections, which are widespread. However, the potential of persistent infections to develop towards pathogenesis remains to be investigated, particularly with respect to the role of host metabolism. To explore the interactions between cellular metabolism and persistent/pathogenic virus infection, we performed untargeted and targeted metabolomic analysis to examine the effects of <i>Cricket paralysis virus</i> (CrPV, <i>Dicistroviridae</i>) in persistently infected silkworm Bm5 cells and acutely infected <i>Drosophila</i> S2 cells. Our previous study (Viruses 2019, 11, 861) established that both glucose and glutamine levels significantly increased during the persistent period of CrPV infection of Bm5 cells, while they decreased steeply during the pathogenic stages. Strikingly, in this study, an almost opposite pattern in change of metabolites was observed during different stages of acute infection of S2 cells. More specifically, a significant decrease in amino acids and carbohydrates was observed prior to pathogenesis, while their abundance significantly increased again during pathogenesis. Our study illustrates the occurrence of diametrically opposite changes in central carbon mechanisms during CrPV infection of S2 and Bm5 cells that is possibly related to the type of infection (acute or persistent) that is triggered by the virus. |
| first_indexed | 2024-03-10T20:45:08Z |
| format | Article |
| id | doaj.art-a7bc0ffdb72a49a98f4dcdbc32ed7b0a |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T20:45:08Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-a7bc0ffdb72a49a98f4dcdbc32ed7b0a2023-11-19T20:21:28ZengMDPI AGViruses1999-49152020-04-0112439310.3390/v12040393Metabolomic Analysis of <i>Cricket paralysis</i> <i>virus</i> Infection in <i>Drosophila</i> S2 Cells Reveals Divergent Effects on Central Carbon Metabolism as Compared with Silkworm Bm5 CellsLuo-Luo Wang0Luc Swevers1Lieven Van Meulebroek2Ivan Meeus3Lynn Vanhaecke4Guy Smagghe5Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaInsect Molecular Genetics and Biotechnology, Institute of Biosciences and Applications, National Centre for Scientific Research “Demokritos”, 153 10 Athens, GreeceLaboratory of Chemical Analysis, Department of Veterinary Public Health and Food Safety, Ghent University, 9000 Ghent, BelgiumDepartment of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, BelgiumLaboratory of Chemical Analysis, Department of Veterinary Public Health and Food Safety, Ghent University, 9000 Ghent, BelgiumDepartment of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, BelgiumHigh-throughput approaches have opened new opportunities for understanding biological processes such as persistent virus infections, which are widespread. However, the potential of persistent infections to develop towards pathogenesis remains to be investigated, particularly with respect to the role of host metabolism. To explore the interactions between cellular metabolism and persistent/pathogenic virus infection, we performed untargeted and targeted metabolomic analysis to examine the effects of <i>Cricket paralysis virus</i> (CrPV, <i>Dicistroviridae</i>) in persistently infected silkworm Bm5 cells and acutely infected <i>Drosophila</i> S2 cells. Our previous study (Viruses 2019, 11, 861) established that both glucose and glutamine levels significantly increased during the persistent period of CrPV infection of Bm5 cells, while they decreased steeply during the pathogenic stages. Strikingly, in this study, an almost opposite pattern in change of metabolites was observed during different stages of acute infection of S2 cells. More specifically, a significant decrease in amino acids and carbohydrates was observed prior to pathogenesis, while their abundance significantly increased again during pathogenesis. Our study illustrates the occurrence of diametrically opposite changes in central carbon mechanisms during CrPV infection of S2 and Bm5 cells that is possibly related to the type of infection (acute or persistent) that is triggered by the virus.https://www.mdpi.com/1999-4915/12/4/393metabolomicspersistent infectionhost metabolism<i>Cricket paralysis virus</i>central carbon metabolism |
| spellingShingle | Luo-Luo Wang Luc Swevers Lieven Van Meulebroek Ivan Meeus Lynn Vanhaecke Guy Smagghe Metabolomic Analysis of <i>Cricket paralysis</i> <i>virus</i> Infection in <i>Drosophila</i> S2 Cells Reveals Divergent Effects on Central Carbon Metabolism as Compared with Silkworm Bm5 Cells Viruses metabolomics persistent infection host metabolism <i>Cricket paralysis virus</i> central carbon metabolism |
| title | Metabolomic Analysis of <i>Cricket paralysis</i> <i>virus</i> Infection in <i>Drosophila</i> S2 Cells Reveals Divergent Effects on Central Carbon Metabolism as Compared with Silkworm Bm5 Cells |
| title_full | Metabolomic Analysis of <i>Cricket paralysis</i> <i>virus</i> Infection in <i>Drosophila</i> S2 Cells Reveals Divergent Effects on Central Carbon Metabolism as Compared with Silkworm Bm5 Cells |
| title_fullStr | Metabolomic Analysis of <i>Cricket paralysis</i> <i>virus</i> Infection in <i>Drosophila</i> S2 Cells Reveals Divergent Effects on Central Carbon Metabolism as Compared with Silkworm Bm5 Cells |
| title_full_unstemmed | Metabolomic Analysis of <i>Cricket paralysis</i> <i>virus</i> Infection in <i>Drosophila</i> S2 Cells Reveals Divergent Effects on Central Carbon Metabolism as Compared with Silkworm Bm5 Cells |
| title_short | Metabolomic Analysis of <i>Cricket paralysis</i> <i>virus</i> Infection in <i>Drosophila</i> S2 Cells Reveals Divergent Effects on Central Carbon Metabolism as Compared with Silkworm Bm5 Cells |
| title_sort | metabolomic analysis of i cricket paralysis i i virus i infection in i drosophila i s2 cells reveals divergent effects on central carbon metabolism as compared with silkworm bm5 cells |
| topic | metabolomics persistent infection host metabolism <i>Cricket paralysis virus</i> central carbon metabolism |
| url | https://www.mdpi.com/1999-4915/12/4/393 |
| work_keys_str_mv | AT luoluowang metabolomicanalysisoficricketparalysisiivirusiinfectioninidrosophilais2cellsrevealsdivergenteffectsoncentralcarbonmetabolismascomparedwithsilkwormbm5cells AT lucswevers metabolomicanalysisoficricketparalysisiivirusiinfectioninidrosophilais2cellsrevealsdivergenteffectsoncentralcarbonmetabolismascomparedwithsilkwormbm5cells AT lievenvanmeulebroek metabolomicanalysisoficricketparalysisiivirusiinfectioninidrosophilais2cellsrevealsdivergenteffectsoncentralcarbonmetabolismascomparedwithsilkwormbm5cells AT ivanmeeus metabolomicanalysisoficricketparalysisiivirusiinfectioninidrosophilais2cellsrevealsdivergenteffectsoncentralcarbonmetabolismascomparedwithsilkwormbm5cells AT lynnvanhaecke metabolomicanalysisoficricketparalysisiivirusiinfectioninidrosophilais2cellsrevealsdivergenteffectsoncentralcarbonmetabolismascomparedwithsilkwormbm5cells AT guysmagghe metabolomicanalysisoficricketparalysisiivirusiinfectioninidrosophilais2cellsrevealsdivergenteffectsoncentralcarbonmetabolismascomparedwithsilkwormbm5cells |