Geroprotective activity of trans-cinnamic acid isolated from the Baikal skullcap (Scutellaria baicalensis)
Trans-cinnamic acid is a phenolic compound with a wide range of bioactive properties, including antioxidant and antibacterial effects. It also has high potential in the food and cosmetic industries. We aimed to isolate trans-cinnamic acid from the Baikal skullcap (Scutellaria baicalensis) and study...
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| Format: | Article |
| Language: | Russian |
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Kemerovo State University
2022-10-01
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| Series: | Техника и технология пищевых производств |
| Subjects: | |
| Online Access: | https://fptt.ru/en/issues/20632/20620/ |
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| author | Anastasiya M. Fedorova Lyubov S. Dyshlyuk Irina S. Milentyeva Anna I. Loseva Olga A. Neverova Mohammed El Amine Khelef |
| author_facet | Anastasiya M. Fedorova Lyubov S. Dyshlyuk Irina S. Milentyeva Anna I. Loseva Olga A. Neverova Mohammed El Amine Khelef |
| author_sort | Anastasiya M. Fedorova |
| collection | DOAJ |
| description | Trans-cinnamic acid is a phenolic compound with a wide range of bioactive properties, including antioxidant and antibacterial effects. It also has high potential in the food and cosmetic industries. We aimed to isolate trans-cinnamic acid from the Baikal skullcap (Scutellaria baicalensis) and study its geroprotective activity on Caenorhabditis elegans nematodes used as a model organism.
Our study objects included the S. baicalensis root culture and its extract, trans-cinnamic acid isolated from the extract, and C. elegans nematodes. Trans-cinnamic acid was isolated by high-performance liquid chromatography. The acid’s geroprotective activity was studied by evaluating its effect at concentrations of 10, 50, 100, and 200 μmol/L on the lifespan, stress resistance, and reproductivity of C. elegans. For the lifespan study, the nematodes were cultivated at 20°C for 61 days. To assess their resistance to oxidative stress, 15 μL of 1M paraquat was added to each well of the plate. Thermal stress resistance was determined by raising the temperature to 33°C. For the reproductivity study, the nematodes were cultivated in the S-medium with the addition of Escherichia coli OP50 and trans-cinnamic acid at required concentrations for 72 h.
The maximum increase in lifespan (9.8%) was observed in the nematodes treated with 50 μmol/L of trans-cinnamic acid. Under oxidative stress, all the concentrations of trans-cinnamic acid increased the survival of nematodes, while under thermal stress, trans-cinnamic acid reduced the percentage of surviving nematodes. At a concentration of 100 μmol/L, trans-cinnamic acid increased the nematodes’ reproduction by 1.48 times.
Based on our data, trans-cinnamic acid isolated from S. baicalensis can be recommended as a bioactive compound with geroprotective activity. However, further research is needed on other model organisms with detailed toxicity studies. |
| first_indexed | 2024-04-12T16:22:03Z |
| format | Article |
| id | doaj.art-a7beef08567f49e0b0f7ee61cac59aa2 |
| institution | Directory Open Access Journal |
| issn | 2074-9414 2313-1748 |
| language | Russian |
| last_indexed | 2024-04-12T16:22:03Z |
| publishDate | 2022-10-01 |
| publisher | Kemerovo State University |
| record_format | Article |
| series | Техника и технология пищевых производств |
| spelling | doaj.art-a7beef08567f49e0b0f7ee61cac59aa22022-12-22T03:25:32ZrusKemerovo State UniversityТехника и технология пищевых производств2074-94142313-17482022-10-0152358259110.21603/2074-9414-2022-3-2388Geroprotective activity of trans-cinnamic acid isolated from the Baikal skullcap (Scutellaria baicalensis)Anastasiya M. Fedorova0https://orcid.org/0000-0002-8071-4411Lyubov S. Dyshlyuk1https://orcid.org/0000-0002-7333-8411Irina S. Milentyeva2https://orcid.org/0000-0002-3536-562XAnna I. Loseva3https://orcid.org/0000-0003-4037-2653Olga A. Neverova4https://orcid.org/0000-0001-7921-0569Mohammed El Amine Khelef5https://orcid.org/0000-0002-9371-7670Kemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaMoscow State University of Food Production, Moscow, RussiaTrans-cinnamic acid is a phenolic compound with a wide range of bioactive properties, including antioxidant and antibacterial effects. It also has high potential in the food and cosmetic industries. We aimed to isolate trans-cinnamic acid from the Baikal skullcap (Scutellaria baicalensis) and study its geroprotective activity on Caenorhabditis elegans nematodes used as a model organism. Our study objects included the S. baicalensis root culture and its extract, trans-cinnamic acid isolated from the extract, and C. elegans nematodes. Trans-cinnamic acid was isolated by high-performance liquid chromatography. The acid’s geroprotective activity was studied by evaluating its effect at concentrations of 10, 50, 100, and 200 μmol/L on the lifespan, stress resistance, and reproductivity of C. elegans. For the lifespan study, the nematodes were cultivated at 20°C for 61 days. To assess their resistance to oxidative stress, 15 μL of 1M paraquat was added to each well of the plate. Thermal stress resistance was determined by raising the temperature to 33°C. For the reproductivity study, the nematodes were cultivated in the S-medium with the addition of Escherichia coli OP50 and trans-cinnamic acid at required concentrations for 72 h. The maximum increase in lifespan (9.8%) was observed in the nematodes treated with 50 μmol/L of trans-cinnamic acid. Under oxidative stress, all the concentrations of trans-cinnamic acid increased the survival of nematodes, while under thermal stress, trans-cinnamic acid reduced the percentage of surviving nematodes. At a concentration of 100 μmol/L, trans-cinnamic acid increased the nematodes’ reproduction by 1.48 times. Based on our data, trans-cinnamic acid isolated from S. baicalensis can be recommended as a bioactive compound with geroprotective activity. However, further research is needed on other model organisms with detailed toxicity studies.https://fptt.ru/en/issues/20632/20620/trans-cinnamic acidroot culturescutellaria baicalensiscaenorhabditis elegansgeroprotectorlife expectancystress resistancereproduction |
| spellingShingle | Anastasiya M. Fedorova Lyubov S. Dyshlyuk Irina S. Milentyeva Anna I. Loseva Olga A. Neverova Mohammed El Amine Khelef Geroprotective activity of trans-cinnamic acid isolated from the Baikal skullcap (Scutellaria baicalensis) Техника и технология пищевых производств trans-cinnamic acid root culture scutellaria baicalensis caenorhabditis elegans geroprotector life expectancy stress resistance reproduction |
| title | Geroprotective activity of trans-cinnamic acid isolated from the Baikal skullcap (Scutellaria baicalensis) |
| title_full | Geroprotective activity of trans-cinnamic acid isolated from the Baikal skullcap (Scutellaria baicalensis) |
| title_fullStr | Geroprotective activity of trans-cinnamic acid isolated from the Baikal skullcap (Scutellaria baicalensis) |
| title_full_unstemmed | Geroprotective activity of trans-cinnamic acid isolated from the Baikal skullcap (Scutellaria baicalensis) |
| title_short | Geroprotective activity of trans-cinnamic acid isolated from the Baikal skullcap (Scutellaria baicalensis) |
| title_sort | geroprotective activity of trans cinnamic acid isolated from the baikal skullcap scutellaria baicalensis |
| topic | trans-cinnamic acid root culture scutellaria baicalensis caenorhabditis elegans geroprotector life expectancy stress resistance reproduction |
| url | https://fptt.ru/en/issues/20632/20620/ |
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