Quercetin Inhibits Biofilm Formation by Decreasing the Production of EPS and Altering the Composition of EPS in Staphylococcus epidermidis
Staphylococcus epidermidis is an opportunistic pathogen, and its biofilm formation ability is an important virulent factor. Quercetin, a typical flavonoid ubiquitously used in dietary supplementation, is known for its antioxidant property, but its anti-biofilm activity against S. epidermidis remains...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.631058/full |
| _version_ | 1823960177033871360 |
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| author | Yongqi Mu Yongqi Mu Hong Zeng Hong Zeng Wei Chen Wei Chen Wei Chen Wei Chen Wei Chen |
| author_facet | Yongqi Mu Yongqi Mu Hong Zeng Hong Zeng Wei Chen Wei Chen Wei Chen Wei Chen Wei Chen |
| author_sort | Yongqi Mu |
| collection | DOAJ |
| description | Staphylococcus epidermidis is an opportunistic pathogen, and its biofilm formation ability is an important virulent factor. Quercetin, a typical flavonoid ubiquitously used in dietary supplementation, is known for its antioxidant property, but its anti-biofilm activity against S. epidermidis remains unknown. In this study, the anti-biofilm activity of quercetin was investigated using S. epidermidis ATCC35984, a strong biofilm-positive strain. An attempt was made to disclose the mechanisms of the anti-biofilm activity of quercetin. S. epidermidis exhibited a less cell surface hydrophobicity after quercetin treatment. Also, quercetin effectively inhibited S. epidermidis cells from adhering to the glass slides. Quercetin downregulated the intercellular adhesion (ica) locus and then polysaccharide intercellular adhesin (PIA) production was reduced. Therefore, S. epidermidis cells became less hydrophobic, which supported quercetin’s anti-biofilm effect. Our study suggests that quercetin from plants be given further attention as a potential anti-biofilm agent against the biofilm formation of S. epidermidis, even biofilm infections of other bacteria. |
| first_indexed | 2024-12-17T16:44:26Z |
| format | Article |
| id | doaj.art-a7c392c8a88c41999d94064678ede4d0 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-17T16:44:26Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a7c392c8a88c41999d94064678ede4d02022-12-21T21:40:47ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-03-011210.3389/fmicb.2021.631058631058Quercetin Inhibits Biofilm Formation by Decreasing the Production of EPS and Altering the Composition of EPS in Staphylococcus epidermidisYongqi Mu0Yongqi Mu1Hong Zeng2Hong Zeng3Wei Chen4Wei Chen5Wei Chen6Wei Chen7Wei Chen8Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar, ChinaCollege of Life Sciences, Tarim University, Alar, ChinaKey Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar, ChinaCollege of Life Sciences, Tarim University, Alar, ChinaKey Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar, ChinaCollege of Life Sciences, Tarim University, Alar, ChinaCollege of Animal Sciences Tarim University, Alar, ChinaKey Laboratory of Tarim Animal Husbandry and Science Technology of Xinjiang Production and Construction Corps, Tarim University, Alar, ChinaEngineering Laboratory for Tarim Animal Diseases Diagnosis and Control of Xinjiang Production and Construction Corps, Tarim University, Alar, ChinaStaphylococcus epidermidis is an opportunistic pathogen, and its biofilm formation ability is an important virulent factor. Quercetin, a typical flavonoid ubiquitously used in dietary supplementation, is known for its antioxidant property, but its anti-biofilm activity against S. epidermidis remains unknown. In this study, the anti-biofilm activity of quercetin was investigated using S. epidermidis ATCC35984, a strong biofilm-positive strain. An attempt was made to disclose the mechanisms of the anti-biofilm activity of quercetin. S. epidermidis exhibited a less cell surface hydrophobicity after quercetin treatment. Also, quercetin effectively inhibited S. epidermidis cells from adhering to the glass slides. Quercetin downregulated the intercellular adhesion (ica) locus and then polysaccharide intercellular adhesin (PIA) production was reduced. Therefore, S. epidermidis cells became less hydrophobic, which supported quercetin’s anti-biofilm effect. Our study suggests that quercetin from plants be given further attention as a potential anti-biofilm agent against the biofilm formation of S. epidermidis, even biofilm infections of other bacteria.https://www.frontiersin.org/articles/10.3389/fmicb.2021.631058/fullquercetinbiofilmhydrophobicityicaPIAStaphylococcus epidermidis |
| spellingShingle | Yongqi Mu Yongqi Mu Hong Zeng Hong Zeng Wei Chen Wei Chen Wei Chen Wei Chen Wei Chen Quercetin Inhibits Biofilm Formation by Decreasing the Production of EPS and Altering the Composition of EPS in Staphylococcus epidermidis Frontiers in Microbiology quercetin biofilm hydrophobicity ica PIA Staphylococcus epidermidis |
| title | Quercetin Inhibits Biofilm Formation by Decreasing the Production of EPS and Altering the Composition of EPS in Staphylococcus epidermidis |
| title_full | Quercetin Inhibits Biofilm Formation by Decreasing the Production of EPS and Altering the Composition of EPS in Staphylococcus epidermidis |
| title_fullStr | Quercetin Inhibits Biofilm Formation by Decreasing the Production of EPS and Altering the Composition of EPS in Staphylococcus epidermidis |
| title_full_unstemmed | Quercetin Inhibits Biofilm Formation by Decreasing the Production of EPS and Altering the Composition of EPS in Staphylococcus epidermidis |
| title_short | Quercetin Inhibits Biofilm Formation by Decreasing the Production of EPS and Altering the Composition of EPS in Staphylococcus epidermidis |
| title_sort | quercetin inhibits biofilm formation by decreasing the production of eps and altering the composition of eps in staphylococcus epidermidis |
| topic | quercetin biofilm hydrophobicity ica PIA Staphylococcus epidermidis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.631058/full |
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