Enteroaggregative Escherichia coli in mid-Norway: A prospective, case control study.
<h4>Background</h4>The use of molecular methods has led to increased detection of Enteroaggregative Escherichia coli (EAEC) in faecal samples. Studies have yielded conflicting results regarding the clinical relevance of this finding. The objective of this study was to investigate the pre...
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| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0301625&type=printable |
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| author | Ingvild Haugan Marit Gudrun Husby Bjørg Skjøtskift Dorothea Aamnes Mostue Andreas Brun Lene Christin Olsen Melanie Rae Simpson Heidi Lange Jan Egil Afset |
| author_facet | Ingvild Haugan Marit Gudrun Husby Bjørg Skjøtskift Dorothea Aamnes Mostue Andreas Brun Lene Christin Olsen Melanie Rae Simpson Heidi Lange Jan Egil Afset |
| author_sort | Ingvild Haugan |
| collection | DOAJ |
| description | <h4>Background</h4>The use of molecular methods has led to increased detection of Enteroaggregative Escherichia coli (EAEC) in faecal samples. Studies have yielded conflicting results regarding the clinical relevance of this finding. The objective of this study was to investigate the prevalence of EAEC in faecal samples from patients with diarrhoea and healthy controls and describe characteristics of EAEC positive persons.<h4>Methods</h4>From March 1st, 2017 to February 28th, 2019, we investigated all consecutive faecal samples from patients with diarrhoea received at the laboratory and collected faecal samples from randomly invited healthy controls from mid-Norway. Real-time multiplex PCR was used for detection of bacterial, viral, and parasitic pathogens. We registered sex, age, urban versus non-urban residency, and travel history for all participants. Statistical analyses were performed with Pearson chi-squared test, Kruskal-Wallis test, and Mann-Whitney U test.<h4>Results</h4>We identified EAEC in 440 of 9487 (4.6%) patients with diarrhoea and 8 of 375 (2.2%) healthy controls. The EAEC prevalence was 19.1% among those with diarrhoea and recent foreign travel and 2.2% in those without travel history independent of diarrhoea. Concomitant pathogens were detected in 64.3% of EAEC-positive patients with diarrhoea. The median age was 28.5 in those with EAEC-positive diarrhoea and 38 in those with EAEC-negative diarrhoea (p <0.01). In patients with diarrhoea, travel was reported in 72% of those with EAEC and concomitant pathogens, and 54% and 12% in those with only EAEC and no EAEC, respectively (p <0.01).<h4>Conclusions</h4>EAEC was a common detection, particularly in patients with diarrhoea and recent international travel, and was found together with other intestinal pathogens in the majority of cases. Our results suggest that domestically acquired EAEC is not associated with diarrhoea. Patients with EAEC-positive diarrhoea and concomitant pathogens were young and often reported recent travel history compared to other patients with diarrhoea. |
| first_indexed | 2024-04-24T06:03:23Z |
| format | Article |
| id | doaj.art-a7c4424017ad4ac5a78147e35d401009 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-24T06:03:23Z |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a7c4424017ad4ac5a78147e35d4010092024-04-23T05:31:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01194e030162510.1371/journal.pone.0301625Enteroaggregative Escherichia coli in mid-Norway: A prospective, case control study.Ingvild HauganMarit Gudrun HusbyBjørg SkjøtskiftDorothea Aamnes MostueAndreas BrunLene Christin OlsenMelanie Rae SimpsonHeidi LangeJan Egil Afset<h4>Background</h4>The use of molecular methods has led to increased detection of Enteroaggregative Escherichia coli (EAEC) in faecal samples. Studies have yielded conflicting results regarding the clinical relevance of this finding. The objective of this study was to investigate the prevalence of EAEC in faecal samples from patients with diarrhoea and healthy controls and describe characteristics of EAEC positive persons.<h4>Methods</h4>From March 1st, 2017 to February 28th, 2019, we investigated all consecutive faecal samples from patients with diarrhoea received at the laboratory and collected faecal samples from randomly invited healthy controls from mid-Norway. Real-time multiplex PCR was used for detection of bacterial, viral, and parasitic pathogens. We registered sex, age, urban versus non-urban residency, and travel history for all participants. Statistical analyses were performed with Pearson chi-squared test, Kruskal-Wallis test, and Mann-Whitney U test.<h4>Results</h4>We identified EAEC in 440 of 9487 (4.6%) patients with diarrhoea and 8 of 375 (2.2%) healthy controls. The EAEC prevalence was 19.1% among those with diarrhoea and recent foreign travel and 2.2% in those without travel history independent of diarrhoea. Concomitant pathogens were detected in 64.3% of EAEC-positive patients with diarrhoea. The median age was 28.5 in those with EAEC-positive diarrhoea and 38 in those with EAEC-negative diarrhoea (p <0.01). In patients with diarrhoea, travel was reported in 72% of those with EAEC and concomitant pathogens, and 54% and 12% in those with only EAEC and no EAEC, respectively (p <0.01).<h4>Conclusions</h4>EAEC was a common detection, particularly in patients with diarrhoea and recent international travel, and was found together with other intestinal pathogens in the majority of cases. Our results suggest that domestically acquired EAEC is not associated with diarrhoea. Patients with EAEC-positive diarrhoea and concomitant pathogens were young and often reported recent travel history compared to other patients with diarrhoea.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0301625&type=printable |
| spellingShingle | Ingvild Haugan Marit Gudrun Husby Bjørg Skjøtskift Dorothea Aamnes Mostue Andreas Brun Lene Christin Olsen Melanie Rae Simpson Heidi Lange Jan Egil Afset Enteroaggregative Escherichia coli in mid-Norway: A prospective, case control study. PLoS ONE |
| title | Enteroaggregative Escherichia coli in mid-Norway: A prospective, case control study. |
| title_full | Enteroaggregative Escherichia coli in mid-Norway: A prospective, case control study. |
| title_fullStr | Enteroaggregative Escherichia coli in mid-Norway: A prospective, case control study. |
| title_full_unstemmed | Enteroaggregative Escherichia coli in mid-Norway: A prospective, case control study. |
| title_short | Enteroaggregative Escherichia coli in mid-Norway: A prospective, case control study. |
| title_sort | enteroaggregative escherichia coli in mid norway a prospective case control study |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0301625&type=printable |
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