SMART: On-Site Rapid Detection of Nucleic Acid from Plants, Animals, and Microorganisms in under 25 Minutes
The rapid on-site nucleic acid detection method is urgently required in many fields. In this study, we report a portable and highly integrated device for DNA detection that combines ultrafast DNA adsorption and rapid DNA amplification. The device, known as silicon film mediated recombinase polymeras...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/1/82 |
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| author | Jun-Yuan Ma Xiao-Fu Wang Cheng Peng Xiao-Yun Chen Xiao-Li Xu Wei Wei Lei Yang Jian Cai Jun-Feng Xu |
| author_facet | Jun-Yuan Ma Xiao-Fu Wang Cheng Peng Xiao-Yun Chen Xiao-Li Xu Wei Wei Lei Yang Jian Cai Jun-Feng Xu |
| author_sort | Jun-Yuan Ma |
| collection | DOAJ |
| description | The rapid on-site nucleic acid detection method is urgently required in many fields. In this study, we report a portable and highly integrated device for DNA detection that combines ultrafast DNA adsorption and rapid DNA amplification. The device, known as silicon film mediated recombinase polymerase amplification (RPA) for nucleic acid detection (SMART), can detect target DNA in less than 25 min from plants, animals, and microbes. Utilizing SMART, transgenic maize was rapidly detected with high selectivity and sensitivity. The sensitivity threshold of the SMART for transgenic maize genomic DNA was 50 copies. The detection results of genuine samples containing plants, animals, and microbes by SMART were consistent with the conventional polymerase chain reaction (PCR) method, demonstrating the high robustness of SMART. Additionally, SMART does not require expensive equipment and is fast, affordable, and user-friendly, making it suited for the broad-scale on-site detection of nucleic acids. |
| first_indexed | 2024-03-09T13:25:32Z |
| format | Article |
| id | doaj.art-a7c46999afaa463dbb9e4b0078e723b5 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T13:25:32Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-a7c46999afaa463dbb9e4b0078e723b52023-11-30T21:25:14ZengMDPI AGBiosensors2079-63742023-01-011318210.3390/bios13010082SMART: On-Site Rapid Detection of Nucleic Acid from Plants, Animals, and Microorganisms in under 25 MinutesJun-Yuan Ma0Xiao-Fu Wang1Cheng Peng2Xiao-Yun Chen3Xiao-Li Xu4Wei Wei5Lei Yang6Jian Cai7Jun-Feng Xu8State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaCollege of Biological and Food Engineering, Fuyang Normal University, Fuyang 236041, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaThe rapid on-site nucleic acid detection method is urgently required in many fields. In this study, we report a portable and highly integrated device for DNA detection that combines ultrafast DNA adsorption and rapid DNA amplification. The device, known as silicon film mediated recombinase polymerase amplification (RPA) for nucleic acid detection (SMART), can detect target DNA in less than 25 min from plants, animals, and microbes. Utilizing SMART, transgenic maize was rapidly detected with high selectivity and sensitivity. The sensitivity threshold of the SMART for transgenic maize genomic DNA was 50 copies. The detection results of genuine samples containing plants, animals, and microbes by SMART were consistent with the conventional polymerase chain reaction (PCR) method, demonstrating the high robustness of SMART. Additionally, SMART does not require expensive equipment and is fast, affordable, and user-friendly, making it suited for the broad-scale on-site detection of nucleic acids.https://www.mdpi.com/2079-6374/13/1/82SMARTon-site detectionultrafast DNA adsorptionRPA |
| spellingShingle | Jun-Yuan Ma Xiao-Fu Wang Cheng Peng Xiao-Yun Chen Xiao-Li Xu Wei Wei Lei Yang Jian Cai Jun-Feng Xu SMART: On-Site Rapid Detection of Nucleic Acid from Plants, Animals, and Microorganisms in under 25 Minutes Biosensors SMART on-site detection ultrafast DNA adsorption RPA |
| title | SMART: On-Site Rapid Detection of Nucleic Acid from Plants, Animals, and Microorganisms in under 25 Minutes |
| title_full | SMART: On-Site Rapid Detection of Nucleic Acid from Plants, Animals, and Microorganisms in under 25 Minutes |
| title_fullStr | SMART: On-Site Rapid Detection of Nucleic Acid from Plants, Animals, and Microorganisms in under 25 Minutes |
| title_full_unstemmed | SMART: On-Site Rapid Detection of Nucleic Acid from Plants, Animals, and Microorganisms in under 25 Minutes |
| title_short | SMART: On-Site Rapid Detection of Nucleic Acid from Plants, Animals, and Microorganisms in under 25 Minutes |
| title_sort | smart on site rapid detection of nucleic acid from plants animals and microorganisms in under 25 minutes |
| topic | SMART on-site detection ultrafast DNA adsorption RPA |
| url | https://www.mdpi.com/2079-6374/13/1/82 |
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