Effects of Expanded Human Adipose Tissue-Derived Mesenchymal Stem Cells on the Viability of Cryopreserved Fat Grafts in the Nude Mouse
<p>Adipose-derived mesenchymal stem cells (AdMSCs) augment the ability to contribute to microvascular remodeling <i>in vivo</i> and to modulate vascular stability in fresh fat grafts. Although cryopreserved adipose tissue is frequently used for soft tissue augmentation, the viabili...
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| Format: | Article |
| Language: | English |
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Ivyspring International Publisher
2011-01-01
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| Series: | International Journal of Medical Sciences |
| Online Access: | http://www.medsci.org/v08p0231.htm |
| _version_ | 1818213440441810944 |
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| author | Myung-Soon Ko, Ji-Youl Jung, Il-Seob Shin, Eun-Wha Choi, Jae-Hoon Kim, Sung Keun Kang, Jeong Chan Ra |
| author_facet | Myung-Soon Ko, Ji-Youl Jung, Il-Seob Shin, Eun-Wha Choi, Jae-Hoon Kim, Sung Keun Kang, Jeong Chan Ra |
| author_sort | Myung-Soon Ko, Ji-Youl Jung, Il-Seob Shin, Eun-Wha Choi, Jae-Hoon Kim, Sung Keun Kang, Jeong Chan Ra |
| collection | DOAJ |
| description | <p>Adipose-derived mesenchymal stem cells (AdMSCs) augment the ability to contribute to microvascular remodeling <i>in vivo</i> and to modulate vascular stability in fresh fat grafts. Although cryopreserved adipose tissue is frequently used for soft tissue augmentation, the viability of the fat graft is poor. The effects of culture-expanded human adipose tissue-derived mesenchymal stem cells (hAdMSCs) on the survival and quality of the cryopreserved fat graft were determined. hAdMSCs from the same donor were mixed with fat tissues cryopreserved at -70°C for 8 weeks and injected subcutaneously into 6-week-old BALB/c-nu nude mice. Graft volume and weight were measured, and histology was evaluated 4 and 15 weeks post-transplantation. The hAdMSC-treated group showed significantly enhanced graft volume and weight. The histological evaluation demonstrated significantly better fat cell integrity compared with the vehicle-treated control 4 weeks post-transplantation. No significant difference in graft weight, volume, or histological parameters was found among the groups 15 weeks post-transplantation. The hAdMSCs enhanced the survival and quality of transplanted cryopreserved fat tissues. Cultured and expanded hAdMSCs have reconstructive capacity in cryopreserved fat grafting by increasing the number of stem cells.</p> |
| first_indexed | 2024-12-12T06:04:19Z |
| format | Article |
| id | doaj.art-a7c6abef141943c49a8540ea1b73db0e |
| institution | Directory Open Access Journal |
| issn | 1449-1907 |
| language | English |
| last_indexed | 2024-12-12T06:04:19Z |
| publishDate | 2011-01-01 |
| publisher | Ivyspring International Publisher |
| record_format | Article |
| series | International Journal of Medical Sciences |
| spelling | doaj.art-a7c6abef141943c49a8540ea1b73db0e2022-12-22T00:35:18ZengIvyspring International PublisherInternational Journal of Medical Sciences1449-19072011-01-0183231238Effects of Expanded Human Adipose Tissue-Derived Mesenchymal Stem Cells on the Viability of Cryopreserved Fat Grafts in the Nude MouseMyung-Soon Ko, Ji-Youl Jung, Il-Seob Shin, Eun-Wha Choi, Jae-Hoon Kim, Sung Keun Kang, Jeong Chan Ra<p>Adipose-derived mesenchymal stem cells (AdMSCs) augment the ability to contribute to microvascular remodeling <i>in vivo</i> and to modulate vascular stability in fresh fat grafts. Although cryopreserved adipose tissue is frequently used for soft tissue augmentation, the viability of the fat graft is poor. The effects of culture-expanded human adipose tissue-derived mesenchymal stem cells (hAdMSCs) on the survival and quality of the cryopreserved fat graft were determined. hAdMSCs from the same donor were mixed with fat tissues cryopreserved at -70°C for 8 weeks and injected subcutaneously into 6-week-old BALB/c-nu nude mice. Graft volume and weight were measured, and histology was evaluated 4 and 15 weeks post-transplantation. The hAdMSC-treated group showed significantly enhanced graft volume and weight. The histological evaluation demonstrated significantly better fat cell integrity compared with the vehicle-treated control 4 weeks post-transplantation. No significant difference in graft weight, volume, or histological parameters was found among the groups 15 weeks post-transplantation. The hAdMSCs enhanced the survival and quality of transplanted cryopreserved fat tissues. Cultured and expanded hAdMSCs have reconstructive capacity in cryopreserved fat grafting by increasing the number of stem cells.</p>http://www.medsci.org/v08p0231.htm |
| spellingShingle | Myung-Soon Ko, Ji-Youl Jung, Il-Seob Shin, Eun-Wha Choi, Jae-Hoon Kim, Sung Keun Kang, Jeong Chan Ra Effects of Expanded Human Adipose Tissue-Derived Mesenchymal Stem Cells on the Viability of Cryopreserved Fat Grafts in the Nude Mouse International Journal of Medical Sciences |
| title | Effects of Expanded Human Adipose Tissue-Derived Mesenchymal Stem Cells on the Viability of Cryopreserved Fat Grafts in the Nude Mouse |
| title_full | Effects of Expanded Human Adipose Tissue-Derived Mesenchymal Stem Cells on the Viability of Cryopreserved Fat Grafts in the Nude Mouse |
| title_fullStr | Effects of Expanded Human Adipose Tissue-Derived Mesenchymal Stem Cells on the Viability of Cryopreserved Fat Grafts in the Nude Mouse |
| title_full_unstemmed | Effects of Expanded Human Adipose Tissue-Derived Mesenchymal Stem Cells on the Viability of Cryopreserved Fat Grafts in the Nude Mouse |
| title_short | Effects of Expanded Human Adipose Tissue-Derived Mesenchymal Stem Cells on the Viability of Cryopreserved Fat Grafts in the Nude Mouse |
| title_sort | effects of expanded human adipose tissue derived mesenchymal stem cells on the viability of cryopreserved fat grafts in the nude mouse |
| url | http://www.medsci.org/v08p0231.htm |
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