Noradrenaline and serotonin-dependent sensitization of MSCs to noradrenaline
Stem and progenitor cells are characterized by peculiar mechanisms of hormonal regulation. Here we describe a protocol of analysis of hormonal cross-talk in adipose tissue derived multipotent mesenchymal stem cells (MSCs). Specifically, cells were treated by a “sensitizing” hormone/neuromediator fol...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | MethodsX |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016124000414 |
| _version_ | 1797326313999040512 |
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| author | Vadim I. Chechekhin Konstantin Yu. Kulebyakin Natalia I. Kalinina Pyotr A. Tyurin-Kuzmin |
| author_facet | Vadim I. Chechekhin Konstantin Yu. Kulebyakin Natalia I. Kalinina Pyotr A. Tyurin-Kuzmin |
| author_sort | Vadim I. Chechekhin |
| collection | DOAJ |
| description | Stem and progenitor cells are characterized by peculiar mechanisms of hormonal regulation. Here we describe a protocol of analysis of hormonal cross-talk in adipose tissue derived multipotent mesenchymal stem cells (MSCs). Specifically, cells were treated by a “sensitizing” hormone/neuromediator followed by the measurement of cellular Ca2+ response to the “readout” hormone after various time intervals. This protocol was successfully used in studies demonstrating a permissive effect of noradrenaline and 5-HT on MSCs sensitivity to noradrenaline, which is a predictive marker of the development of obesity-associated arterial hypertension. |
| first_indexed | 2024-03-08T06:21:35Z |
| format | Article |
| id | doaj.art-a7c6cbc542cf4ea090fa4c5991f4a230 |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-03-08T06:21:35Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-a7c6cbc542cf4ea090fa4c5991f4a2302024-02-04T04:44:57ZengElsevierMethodsX2215-01612024-06-0112102587Noradrenaline and serotonin-dependent sensitization of MSCs to noradrenalineVadim I. Chechekhin0Konstantin Yu. Kulebyakin1Natalia I. Kalinina2Pyotr A. Tyurin-Kuzmin3Department of Biochemistry and Regenerative Biomedicine, Faculty of Medicine, Lomonosov Moscow State University, Moscow, 119991, RussiaDepartment of Biochemistry and Regenerative Biomedicine, Faculty of Medicine, Lomonosov Moscow State University, Moscow, 119991, RussiaDepartment of Biochemistry and Regenerative Biomedicine, Faculty of Medicine, Lomonosov Moscow State University, Moscow, 119991, RussiaCorresponding author.; Department of Biochemistry and Regenerative Biomedicine, Faculty of Medicine, Lomonosov Moscow State University, Moscow, 119991, RussiaStem and progenitor cells are characterized by peculiar mechanisms of hormonal regulation. Here we describe a protocol of analysis of hormonal cross-talk in adipose tissue derived multipotent mesenchymal stem cells (MSCs). Specifically, cells were treated by a “sensitizing” hormone/neuromediator followed by the measurement of cellular Ca2+ response to the “readout” hormone after various time intervals. This protocol was successfully used in studies demonstrating a permissive effect of noradrenaline and 5-HT on MSCs sensitivity to noradrenaline, which is a predictive marker of the development of obesity-associated arterial hypertension.http://www.sciencedirect.com/science/article/pii/S2215016124000414NoradrenalineSerotoninMesenchymal stem cellsAdipose tissueObesityObesity-associated hypertension |
| spellingShingle | Vadim I. Chechekhin Konstantin Yu. Kulebyakin Natalia I. Kalinina Pyotr A. Tyurin-Kuzmin Noradrenaline and serotonin-dependent sensitization of MSCs to noradrenaline MethodsX Noradrenaline Serotonin Mesenchymal stem cells Adipose tissue Obesity Obesity-associated hypertension |
| title | Noradrenaline and serotonin-dependent sensitization of MSCs to noradrenaline |
| title_full | Noradrenaline and serotonin-dependent sensitization of MSCs to noradrenaline |
| title_fullStr | Noradrenaline and serotonin-dependent sensitization of MSCs to noradrenaline |
| title_full_unstemmed | Noradrenaline and serotonin-dependent sensitization of MSCs to noradrenaline |
| title_short | Noradrenaline and serotonin-dependent sensitization of MSCs to noradrenaline |
| title_sort | noradrenaline and serotonin dependent sensitization of mscs to noradrenaline |
| topic | Noradrenaline Serotonin Mesenchymal stem cells Adipose tissue Obesity Obesity-associated hypertension |
| url | http://www.sciencedirect.com/science/article/pii/S2215016124000414 |
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