CD20 positivity in classical Hodgkin′s lymphoma: Diagnostic challenge or targeting opportunity
<b>Background:</b> It is now well established that Hodgkin cells are clonal B cells with a CD30 and CD15 phenotype. However, on immunohistochemistry, in our experience and the experience of others, CD20 positivity in an otherwise typical classical Hodgkin′s Lymphoma is not un...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2009-01-01
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| Series: | Indian Journal of Pathology and Microbiology |
| Subjects: | |
| Online Access: | http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2009;volume=52;issue=1;spage=6;epage=9;aulast=Barakzai |
| _version_ | 1828895902866079744 |
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| author | Barakzai Muhammad Pervez Shahid |
| author_facet | Barakzai Muhammad Pervez Shahid |
| author_sort | Barakzai Muhammad |
| collection | DOAJ |
| description | <b>Background:</b> It is now well established that Hodgkin cells are clonal B cells with a CD30 and CD15 phenotype. However, on immunohistochemistry, in our experience and the experience of others, CD20 positivity in an otherwise typical classical Hodgkin′s Lymphoma is not uncommon and if associated with CD15 negativity poses a potential diagnostic trap and is likely to be called B-NHL. <b> Objective: </b> To assess the frequency of B-cell related antigens CD20 and CD79a in classical Hodgkin′s Lymphoma. <b> Materials and Methods: </b> A total of 91 consecutive cases of classical Hodgkin′s Lymphoma were analyzed for co-expression of CD20 and CD79a. Both males and females of all ages were included in this study. All cases of nodular lymphocyte predominant Hodgkin′s Lymphoma were excluded. All the cases were stained with a panel of antibodies including LCA, CD20, CD79a, CD30, CD15, CD3, EMA and Alk. Protein. <b> Results: </b> All 91 cases of classical Hodgkin′s Lymphoma showed negativity for LCA and positivity for CD30. Eighteen cases (19.8%) showed distinct membrane staining with CD20 in most of the large atypical cells. However, out of these, only 7 cases (7.7%) showed CD79a co-expression, which was largely focal. CD15 negativity with CD20 positivity was seen in 7 (7.7%) cases of otherwise typical classical Hodgkin′s Lymphoma. <b> Conclusions/Recommendations:</b> CD20 expression is frequent in classical Hodgkin′s Lymphoma and our results are in consensus with reported literature on this subject. In these cases, LCA negativity of large cells was extremely useful in clinching the right diagnosis. |
| first_indexed | 2024-12-13T14:34:09Z |
| format | Article |
| id | doaj.art-a7c8083442284d4badf71a1cb832bf05 |
| institution | Directory Open Access Journal |
| issn | 0377-4929 |
| language | English |
| last_indexed | 2024-12-13T14:34:09Z |
| publishDate | 2009-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Pathology and Microbiology |
| spelling | doaj.art-a7c8083442284d4badf71a1cb832bf052022-12-21T23:41:45ZengWolters Kluwer Medknow PublicationsIndian Journal of Pathology and Microbiology0377-49292009-01-0152169CD20 positivity in classical Hodgkin′s lymphoma: Diagnostic challenge or targeting opportunityBarakzai MuhammadPervez Shahid<b>Background:</b> It is now well established that Hodgkin cells are clonal B cells with a CD30 and CD15 phenotype. However, on immunohistochemistry, in our experience and the experience of others, CD20 positivity in an otherwise typical classical Hodgkin′s Lymphoma is not uncommon and if associated with CD15 negativity poses a potential diagnostic trap and is likely to be called B-NHL. <b> Objective: </b> To assess the frequency of B-cell related antigens CD20 and CD79a in classical Hodgkin′s Lymphoma. <b> Materials and Methods: </b> A total of 91 consecutive cases of classical Hodgkin′s Lymphoma were analyzed for co-expression of CD20 and CD79a. Both males and females of all ages were included in this study. All cases of nodular lymphocyte predominant Hodgkin′s Lymphoma were excluded. All the cases were stained with a panel of antibodies including LCA, CD20, CD79a, CD30, CD15, CD3, EMA and Alk. Protein. <b> Results: </b> All 91 cases of classical Hodgkin′s Lymphoma showed negativity for LCA and positivity for CD30. Eighteen cases (19.8%) showed distinct membrane staining with CD20 in most of the large atypical cells. However, out of these, only 7 cases (7.7%) showed CD79a co-expression, which was largely focal. CD15 negativity with CD20 positivity was seen in 7 (7.7%) cases of otherwise typical classical Hodgkin′s Lymphoma. <b> Conclusions/Recommendations:</b> CD20 expression is frequent in classical Hodgkin′s Lymphoma and our results are in consensus with reported literature on this subject. In these cases, LCA negativity of large cells was extremely useful in clinching the right diagnosis.http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2009;volume=52;issue=1;spage=6;epage=9;aulast=BarakzaiHodgkin′s lymphomaimmunohistochemistry |
| spellingShingle | Barakzai Muhammad Pervez Shahid CD20 positivity in classical Hodgkin′s lymphoma: Diagnostic challenge or targeting opportunity Indian Journal of Pathology and Microbiology Hodgkin′s lymphoma immunohistochemistry |
| title | CD20 positivity in classical Hodgkin′s lymphoma: Diagnostic challenge or targeting opportunity |
| title_full | CD20 positivity in classical Hodgkin′s lymphoma: Diagnostic challenge or targeting opportunity |
| title_fullStr | CD20 positivity in classical Hodgkin′s lymphoma: Diagnostic challenge or targeting opportunity |
| title_full_unstemmed | CD20 positivity in classical Hodgkin′s lymphoma: Diagnostic challenge or targeting opportunity |
| title_short | CD20 positivity in classical Hodgkin′s lymphoma: Diagnostic challenge or targeting opportunity |
| title_sort | cd20 positivity in classical hodgkin x2032 s lymphoma diagnostic challenge or targeting opportunity |
| topic | Hodgkin′s lymphoma immunohistochemistry |
| url | http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2009;volume=52;issue=1;spage=6;epage=9;aulast=Barakzai |
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