Feline Gastrointestinal Eosinophilic Sclerosing Fibroplasia—Extracellular Matrix Proteins and TGF-β1 Immunoexpression
Feline gastrointestinal eosinophilic sclerosing fibroplasia (FGESF) has been described as an inflammatory disorder with an eosinophilic component with etiopathogenesis that is still unknown. Sixteen intestinal samples from two veterinary diagnostic services (2014–2017) were included in the study. A...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/6/291 |
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| author | Néstor Porras Agustín Rebollada-Merino Fernando Rodríguez-Franco Andrés Calvo-Ibbitson Antonio Rodríguez-Bertos |
| author_facet | Néstor Porras Agustín Rebollada-Merino Fernando Rodríguez-Franco Andrés Calvo-Ibbitson Antonio Rodríguez-Bertos |
| author_sort | Néstor Porras |
| collection | DOAJ |
| description | Feline gastrointestinal eosinophilic sclerosing fibroplasia (FGESF) has been described as an inflammatory disorder with an eosinophilic component with etiopathogenesis that is still unknown. Sixteen intestinal samples from two veterinary diagnostic services (2014–2017) were included in the study. A histopathological criterion classified the cases into three grades (mild, moderate, and severe) according to the distribution of the lesions and the course. An immunohistochemical study of collagen I, collagen III, fibronectin, and transforming growth factor β1 (TGF-β1) was performed in each case. An immunohistochemical study of mild grades shows greater collagen III immunoexpression, compared to collagen I and fibronectin, which suggests an “early” stage of fibrosis. In more intense grades, an increased immunoexpression of collagen I, compared to collagen III, suggests a “late” stage of fibrosis. Otherwise, the highest expression of TGF-β1 was observed in the moderate phase, due to the high proliferation of reactive fibroblast and intense inflammation. The results suggest that the inflammatory infiltrate is the trigger for the elevation in TGF-β1, altering the collagen type III:I ratio. In conclusion, immunohistochemical studies can be a very useful method in diagnosing cases of FGESF of mild grades and could help to apply a differential diagnosis regarding feline eosinophilic chronic enteritis (CEE) in the context of inflammatory bowel disease (IBD). |
| first_indexed | 2024-03-09T22:16:30Z |
| format | Article |
| id | doaj.art-a7cd0157192d496793eecff66101247c |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T22:16:30Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-a7cd0157192d496793eecff66101247c2023-11-23T19:23:28ZengMDPI AGVeterinary Sciences2306-73812022-06-019629110.3390/vetsci9060291Feline Gastrointestinal Eosinophilic Sclerosing Fibroplasia—Extracellular Matrix Proteins and TGF-β1 ImmunoexpressionNéstor Porras0Agustín Rebollada-Merino1Fernando Rodríguez-Franco2Andrés Calvo-Ibbitson3Antonio Rodríguez-Bertos4VISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainDepartment of Internal Medicine and Animal Surgery, Faculty of Veterinary Medicine, Complutense University of Madrid, 28040 Madrid, SpainVetPatólogos, Av. Isabel de Farnesio, 27, 28660 Boadilla del Monte, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainFeline gastrointestinal eosinophilic sclerosing fibroplasia (FGESF) has been described as an inflammatory disorder with an eosinophilic component with etiopathogenesis that is still unknown. Sixteen intestinal samples from two veterinary diagnostic services (2014–2017) were included in the study. A histopathological criterion classified the cases into three grades (mild, moderate, and severe) according to the distribution of the lesions and the course. An immunohistochemical study of collagen I, collagen III, fibronectin, and transforming growth factor β1 (TGF-β1) was performed in each case. An immunohistochemical study of mild grades shows greater collagen III immunoexpression, compared to collagen I and fibronectin, which suggests an “early” stage of fibrosis. In more intense grades, an increased immunoexpression of collagen I, compared to collagen III, suggests a “late” stage of fibrosis. Otherwise, the highest expression of TGF-β1 was observed in the moderate phase, due to the high proliferation of reactive fibroblast and intense inflammation. The results suggest that the inflammatory infiltrate is the trigger for the elevation in TGF-β1, altering the collagen type III:I ratio. In conclusion, immunohistochemical studies can be a very useful method in diagnosing cases of FGESF of mild grades and could help to apply a differential diagnosis regarding feline eosinophilic chronic enteritis (CEE) in the context of inflammatory bowel disease (IBD).https://www.mdpi.com/2306-7381/9/6/291collagenfeline gastrointestinal eosinophilic sclerosing fibroplasiafibronectinintestinal fibrosisimmunohistochemistryTGF-β1 |
| spellingShingle | Néstor Porras Agustín Rebollada-Merino Fernando Rodríguez-Franco Andrés Calvo-Ibbitson Antonio Rodríguez-Bertos Feline Gastrointestinal Eosinophilic Sclerosing Fibroplasia—Extracellular Matrix Proteins and TGF-β1 Immunoexpression Veterinary Sciences collagen feline gastrointestinal eosinophilic sclerosing fibroplasia fibronectin intestinal fibrosis immunohistochemistry TGF-β1 |
| title | Feline Gastrointestinal Eosinophilic Sclerosing Fibroplasia—Extracellular Matrix Proteins and TGF-β1 Immunoexpression |
| title_full | Feline Gastrointestinal Eosinophilic Sclerosing Fibroplasia—Extracellular Matrix Proteins and TGF-β1 Immunoexpression |
| title_fullStr | Feline Gastrointestinal Eosinophilic Sclerosing Fibroplasia—Extracellular Matrix Proteins and TGF-β1 Immunoexpression |
| title_full_unstemmed | Feline Gastrointestinal Eosinophilic Sclerosing Fibroplasia—Extracellular Matrix Proteins and TGF-β1 Immunoexpression |
| title_short | Feline Gastrointestinal Eosinophilic Sclerosing Fibroplasia—Extracellular Matrix Proteins and TGF-β1 Immunoexpression |
| title_sort | feline gastrointestinal eosinophilic sclerosing fibroplasia extracellular matrix proteins and tgf β1 immunoexpression |
| topic | collagen feline gastrointestinal eosinophilic sclerosing fibroplasia fibronectin intestinal fibrosis immunohistochemistry TGF-β1 |
| url | https://www.mdpi.com/2306-7381/9/6/291 |
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