Complementary Transcriptome and Proteome Analyses Provide Insight into the Floral Transition in Bamboo (<i>Dendrocalamus latiflorus</i> Munro)
Most woody bamboos bloom only once after long vegetative growth phases and die immediately afterwards. It is difficult, however, to determine the timing of the floral transition, as little information is available on the molecular mechanism of plant maturity in bamboos. To uncover the bamboo floral...
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MDPI AG
2020-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/22/8430 |
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| author | Xiaoyan Wang Yujiao Wang Guoqian Yang Lei Zhao Xuemei Zhang Dezhu Li Zhenhua Guo |
| author_facet | Xiaoyan Wang Yujiao Wang Guoqian Yang Lei Zhao Xuemei Zhang Dezhu Li Zhenhua Guo |
| author_sort | Xiaoyan Wang |
| collection | DOAJ |
| description | Most woody bamboos bloom only once after long vegetative growth phases and die immediately afterwards. It is difficult, however, to determine the timing of the floral transition, as little information is available on the molecular mechanism of plant maturity in bamboos. To uncover the bamboo floral transition mechanism, its morpho-physiological characteristics, transcriptomes and large-scale quantitative proteomes were investigated in leaves which were collected at different stages during floral transition in a woody bamboo, <i>Dendrocalamus latiflorus.</i> We identified many flowering time-associated genes and the continued increase and decrease genes were screened as flowering biomarker genes (e.g., the <i>MADS14</i> and <i>bHLH13</i> genes). These different genes were assigned to specific metabolic pathways by the Kyoto Encyclopedia of Genes and Genomes (KEGG). And the photoperiod pathways depending on the circadian rhythm may play an essential role in the bamboo floral transition. In addition, a total of 721 differently expressed proteins of leaves from the vegetative-to-reproductive stages were identified. Fifty-five genes were specifically differentially expressed at both the transcriptomic and proteomic levels, including genes related to photosynthesis and nucleotide sugar, which may be involved in the floral transition. This work provides insights into bamboo flowers and the management of forest breeding. |
| first_indexed | 2024-03-10T14:58:07Z |
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| id | doaj.art-a7d29a21a7dd40ab813e2db8dea20ae5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:58:07Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a7d29a21a7dd40ab813e2db8dea20ae52023-11-20T20:24:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-012122843010.3390/ijms21228430Complementary Transcriptome and Proteome Analyses Provide Insight into the Floral Transition in Bamboo (<i>Dendrocalamus latiflorus</i> Munro)Xiaoyan Wang0Yujiao Wang1Guoqian Yang2Lei Zhao3Xuemei Zhang4Dezhu Li5Zhenhua Guo6Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaMost woody bamboos bloom only once after long vegetative growth phases and die immediately afterwards. It is difficult, however, to determine the timing of the floral transition, as little information is available on the molecular mechanism of plant maturity in bamboos. To uncover the bamboo floral transition mechanism, its morpho-physiological characteristics, transcriptomes and large-scale quantitative proteomes were investigated in leaves which were collected at different stages during floral transition in a woody bamboo, <i>Dendrocalamus latiflorus.</i> We identified many flowering time-associated genes and the continued increase and decrease genes were screened as flowering biomarker genes (e.g., the <i>MADS14</i> and <i>bHLH13</i> genes). These different genes were assigned to specific metabolic pathways by the Kyoto Encyclopedia of Genes and Genomes (KEGG). And the photoperiod pathways depending on the circadian rhythm may play an essential role in the bamboo floral transition. In addition, a total of 721 differently expressed proteins of leaves from the vegetative-to-reproductive stages were identified. Fifty-five genes were specifically differentially expressed at both the transcriptomic and proteomic levels, including genes related to photosynthesis and nucleotide sugar, which may be involved in the floral transition. This work provides insights into bamboo flowers and the management of forest breeding.https://www.mdpi.com/1422-0067/21/22/8430floral transitioniTRAQbiomarker genemRNA-protein correlationbamboo |
| spellingShingle | Xiaoyan Wang Yujiao Wang Guoqian Yang Lei Zhao Xuemei Zhang Dezhu Li Zhenhua Guo Complementary Transcriptome and Proteome Analyses Provide Insight into the Floral Transition in Bamboo (<i>Dendrocalamus latiflorus</i> Munro) International Journal of Molecular Sciences floral transition iTRAQ biomarker gene mRNA-protein correlation bamboo |
| title | Complementary Transcriptome and Proteome Analyses Provide Insight into the Floral Transition in Bamboo (<i>Dendrocalamus latiflorus</i> Munro) |
| title_full | Complementary Transcriptome and Proteome Analyses Provide Insight into the Floral Transition in Bamboo (<i>Dendrocalamus latiflorus</i> Munro) |
| title_fullStr | Complementary Transcriptome and Proteome Analyses Provide Insight into the Floral Transition in Bamboo (<i>Dendrocalamus latiflorus</i> Munro) |
| title_full_unstemmed | Complementary Transcriptome and Proteome Analyses Provide Insight into the Floral Transition in Bamboo (<i>Dendrocalamus latiflorus</i> Munro) |
| title_short | Complementary Transcriptome and Proteome Analyses Provide Insight into the Floral Transition in Bamboo (<i>Dendrocalamus latiflorus</i> Munro) |
| title_sort | complementary transcriptome and proteome analyses provide insight into the floral transition in bamboo i dendrocalamus latiflorus i munro |
| topic | floral transition iTRAQ biomarker gene mRNA-protein correlation bamboo |
| url | https://www.mdpi.com/1422-0067/21/22/8430 |
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