Virus-Induced Gene Silencing (VIGS) in Chinese Jujube
Virus-induced gene silencing (VIGS) is a fast and efficient method for assaying gene function in plants. At present, the VIGS system mediated by Tobacco rattle virus (TRV) has been successfully practiced in some species such as cotton and tomato. However, little research of VIGS systems has been rep...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/12/11/2115 |
| _version_ | 1797596940886605824 |
|---|---|
| author | Yao Zhang Nazi Niu Shijia Li Yin Liu Chaoling Xue Huibin Wang Mengjun Liu Jin Zhao |
| author_facet | Yao Zhang Nazi Niu Shijia Li Yin Liu Chaoling Xue Huibin Wang Mengjun Liu Jin Zhao |
| author_sort | Yao Zhang |
| collection | DOAJ |
| description | Virus-induced gene silencing (VIGS) is a fast and efficient method for assaying gene function in plants. At present, the VIGS system mediated by Tobacco rattle virus (TRV) has been successfully practiced in some species such as cotton and tomato. However, little research of VIGS systems has been reported in woody plants, nor in Chinese jujube. In this study, the TRV-VIGS system of jujube was firstly investigated. The jujube seedlings were grown in a greenhouse with a 16 h light/8 h dark cycle at 23 °C. After the cotyledon was fully unfolded, <i>Agrobacterium</i> mixture containing pTRV1 and pTRV2-ZjCLA with OD<sub>600</sub> = 1.5 was injected into cotyledon. After 15 days, the new leaves of jujube seedlings showed obvious photo-bleaching symptoms and significantly decreased expression of <i>ZjCLA</i>, indicating that the TRV-VIGS system had successfully functioned on jujube. Moreover, it found that two injections on jujube cotyledon could induce higher silencing efficiency than once injection. A similar silencing effect was then also verified in another gene, <i>ZjPDS</i>. These results indicate that the TRV-VIGS system in Chinese jujube has been successfully established and can be applied to evaluate gene function, providing a breakthrough in gene function verification methods. |
| first_indexed | 2024-03-11T02:59:55Z |
| format | Article |
| id | doaj.art-a7d57a4478ed4d97a21458dae1046c47 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T02:59:55Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-a7d57a4478ed4d97a21458dae1046c472023-11-18T08:23:34ZengMDPI AGPlants2223-77472023-05-011211211510.3390/plants12112115Virus-Induced Gene Silencing (VIGS) in Chinese JujubeYao Zhang0Nazi Niu1Shijia Li2Yin Liu3Chaoling Xue4Huibin Wang5Mengjun Liu6Jin Zhao7College of Life Science, Hebei Agricultural University, Baoding 071000, ChinaCollege of Life Science, Hebei Agricultural University, Baoding 071000, ChinaCollege of Life Science, Hebei Agricultural University, Baoding 071000, ChinaCollege of Life Science, Hebei Agricultural University, Baoding 071000, ChinaCollege of Life Science, Hebei Agricultural University, Baoding 071000, ChinaCollege of Life Science, Hebei Agricultural University, Baoding 071000, ChinaResearch Center of Chinese Jujube, Hebei Agricultural University, Baoding 071000, ChinaCollege of Life Science, Hebei Agricultural University, Baoding 071000, ChinaVirus-induced gene silencing (VIGS) is a fast and efficient method for assaying gene function in plants. At present, the VIGS system mediated by Tobacco rattle virus (TRV) has been successfully practiced in some species such as cotton and tomato. However, little research of VIGS systems has been reported in woody plants, nor in Chinese jujube. In this study, the TRV-VIGS system of jujube was firstly investigated. The jujube seedlings were grown in a greenhouse with a 16 h light/8 h dark cycle at 23 °C. After the cotyledon was fully unfolded, <i>Agrobacterium</i> mixture containing pTRV1 and pTRV2-ZjCLA with OD<sub>600</sub> = 1.5 was injected into cotyledon. After 15 days, the new leaves of jujube seedlings showed obvious photo-bleaching symptoms and significantly decreased expression of <i>ZjCLA</i>, indicating that the TRV-VIGS system had successfully functioned on jujube. Moreover, it found that two injections on jujube cotyledon could induce higher silencing efficiency than once injection. A similar silencing effect was then also verified in another gene, <i>ZjPDS</i>. These results indicate that the TRV-VIGS system in Chinese jujube has been successfully established and can be applied to evaluate gene function, providing a breakthrough in gene function verification methods.https://www.mdpi.com/2223-7747/12/11/2115virus-induced gene silencingtobacco rattle virusChinese jujubepTRV2-ZjCLAtwice injectionsgene function |
| spellingShingle | Yao Zhang Nazi Niu Shijia Li Yin Liu Chaoling Xue Huibin Wang Mengjun Liu Jin Zhao Virus-Induced Gene Silencing (VIGS) in Chinese Jujube Plants virus-induced gene silencing tobacco rattle virus Chinese jujube pTRV2-ZjCLA twice injections gene function |
| title | Virus-Induced Gene Silencing (VIGS) in Chinese Jujube |
| title_full | Virus-Induced Gene Silencing (VIGS) in Chinese Jujube |
| title_fullStr | Virus-Induced Gene Silencing (VIGS) in Chinese Jujube |
| title_full_unstemmed | Virus-Induced Gene Silencing (VIGS) in Chinese Jujube |
| title_short | Virus-Induced Gene Silencing (VIGS) in Chinese Jujube |
| title_sort | virus induced gene silencing vigs in chinese jujube |
| topic | virus-induced gene silencing tobacco rattle virus Chinese jujube pTRV2-ZjCLA twice injections gene function |
| url | https://www.mdpi.com/2223-7747/12/11/2115 |
| work_keys_str_mv | AT yaozhang virusinducedgenesilencingvigsinchinesejujube AT naziniu virusinducedgenesilencingvigsinchinesejujube AT shijiali virusinducedgenesilencingvigsinchinesejujube AT yinliu virusinducedgenesilencingvigsinchinesejujube AT chaolingxue virusinducedgenesilencingvigsinchinesejujube AT huibinwang virusinducedgenesilencingvigsinchinesejujube AT mengjunliu virusinducedgenesilencingvigsinchinesejujube AT jinzhao virusinducedgenesilencingvigsinchinesejujube |