| Summary: | Bone morphogenetic proteins (BMPs) are the structurally similar and highly conserved type of functional proteins that play an important role in hair follicle growth and development. <i>BMP7</i> was a differentially expressed gene in different patterns of Hu sheep lambskin identified using Agilent microarray. Since hair follicle is the basis of pattern formation of lambskin, and its growth and development is governed by dermal papilla cells (DPCs), to clarify the role of <i>BMP7</i> and hair follicle, our study was designed to investigate the regulation between <i>BMP7</i> and DPCs. Firstly, the CDS region of <i>BMP7</i> was cloned by 3’Race and PCR in Hu sheep and performed serious of bioinformatic analysis. Then, the effects of <i>BMP7</i> on DPCs were analyzed after overexpression and interference of <i>BMP7</i> in dermal papilla cells by CCK8, EdU, and PI assay. Additionally, qPCR was also conducted to clarify the relationship between <i>BMP7</i> and the TGF-β/Smad signaling pathway. A total of 1296 bp of the <i>BMP7</i> CDS region sequence was sucessfully cloned in Hu sheep, encoding a signal peptide of 431 amino acids, molecular weight was 49,316.9 Da and the isoelectric point (Pi) was 7.75. Nucleotide sequencing analysis of <i>BMP7</i> revealed that Hu sheep had high homology with <i>Bos taurus, Homo sapiens,</i> and <i>Canis lupus familiaris</i>. Structure domain prediction showed that TGF-β superfamily domain exist between 330th–431th amino acid, <i>BMP7</i> protein is a secreted protein. In <i>BMP7</i> up-regulated DPCs, DPCs proliferation rate and cell cycle were significantly higher than that of NC group (<i>p</i> < 0.05). Meanwhile, the expression level of <i>Smad3</i>, <i>Smad4</i>, <i>Samd6</i>, and <i>TGF-β1</i> in TGF-β/Smad signaling pathway were significantly lower than that in NC group (<i>p</i> < 0.05). In <i>BMP7</i> down-regulated DPCs, it presented the opposite result. In conclusion, our study showed that <i>BMP7</i> had a positive effect on DPCs by accelerating the proliferation and cell cycle of DPCs, and hypothesized that regulate hair follicles growth and development via TGF-β/Smad signaling pathway. These findings may provide a synergistic target for the subsequent research of hair follicle growth and development.
|
|---|