Simultaneous Carriage of mcr-1 and Other Antimicrobial Resistance Determinants in Escherichia coli From Poultry
The use of antimicrobial growth promoters (AGPs) in sub-therapeutic doses for long periods promotes the selection of resistant microorganisms and the subsequent risk of spreading this resistance to the human population and the environment. Global concern about antimicrobial resistance development an...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01679/full |
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| author | Johana E. Dominguez Johana E. Dominguez Leandro M. Redondo Leandro M. Redondo Roque A. Figueroa Espinosa Roque A. Figueroa Espinosa Daniela Cejas Daniela Cejas Gabriel O. Gutkind Gabriel O. Gutkind Pablo A. Chacana José A. Di Conza José A. Di Conza Mariano E. Fernández Miyakawa Mariano E. Fernández Miyakawa |
| author_facet | Johana E. Dominguez Johana E. Dominguez Leandro M. Redondo Leandro M. Redondo Roque A. Figueroa Espinosa Roque A. Figueroa Espinosa Daniela Cejas Daniela Cejas Gabriel O. Gutkind Gabriel O. Gutkind Pablo A. Chacana José A. Di Conza José A. Di Conza Mariano E. Fernández Miyakawa Mariano E. Fernández Miyakawa |
| author_sort | Johana E. Dominguez |
| collection | DOAJ |
| description | The use of antimicrobial growth promoters (AGPs) in sub-therapeutic doses for long periods promotes the selection of resistant microorganisms and the subsequent risk of spreading this resistance to the human population and the environment. Global concern about antimicrobial resistance development and transference of resistance genes from animal to human has been rising. The goal of our research was to evaluate the susceptibility pattern to different classes of antimicrobials of colistin-resistant Escherichia coli from poultry production systems that use AGPs, and characterize the resistance determinants associated to transferable platforms. E. coli strains (n = 41) were obtained from fecal samples collected from typical Argentine commercial broiler farms and susceptibility for 23 antimicrobials, relevant for human or veterinary medicine, was determined. Isolates were tested by PCR for the presence of mcr-1, extended spectrum β-lactamase encoding genes and plasmid-mediated quinolone resistance (PMQR) coding genes. Conjugation and susceptibility patterns of the transconjugant studies were performed. ERIC-PCR and REP-PCR analysis showed a high diversity of the isolates. Resistance to several antimicrobials was determined and all colistin-resistant isolates harbored the mcr-1 gene. CTX-M-2 cefotaximase was the main mechanism responsible for third generation cephalosporins resistance, and PMQR determinants were also identified. In addition, co-transference of the qnrB determinant on the mcr-1-positive transconjugants was corroborated, which suggests that these resistance genes are likely to be located in the same plasmid. In this work a wide range of antimicrobial resistance mechanisms were identified in E. coli strains isolated from the environment of healthy chickens highlighting the risk of antimicrobial abuse/misuse in animals under intensive production systems and its consequences for public health. |
| first_indexed | 2024-12-24T05:37:10Z |
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| issn | 1664-302X |
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| last_indexed | 2024-12-24T05:37:10Z |
| publishDate | 2018-07-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-a7dca691229943bcae01b44af733e9b42022-12-21T17:12:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-07-01910.3389/fmicb.2018.01679356016Simultaneous Carriage of mcr-1 and Other Antimicrobial Resistance Determinants in Escherichia coli From PoultryJohana E. Dominguez0Johana E. Dominguez1Leandro M. Redondo2Leandro M. Redondo3Roque A. Figueroa Espinosa4Roque A. Figueroa Espinosa5Daniela Cejas6Daniela Cejas7Gabriel O. Gutkind8Gabriel O. Gutkind9Pablo A. Chacana10José A. Di Conza11José A. Di Conza12Mariano E. Fernández Miyakawa13Mariano E. Fernández Miyakawa14Laboratorio de Bacteriología General, Instituto de Patobiología, Centro Nacional de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Tecnológicas, Buenos Aires, ArgentinaLaboratorio de Bacteriología General, Instituto de Patobiología, Centro Nacional de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Tecnológicas, Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Tecnológicas, Buenos Aires, ArgentinaLaboratorio de Resistencia Bacteriana, Cátedra de Microbiología, Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Tecnológicas, Buenos Aires, ArgentinaLaboratorio de Resistencia Bacteriana, Cátedra de Microbiología, Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Tecnológicas, Buenos Aires, ArgentinaLaboratorio de Resistencia Bacteriana, Cátedra de Microbiología, Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Buenos Aires, ArgentinaLaboratorio de Bacteriología General, Instituto de Patobiología, Centro Nacional de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Tecnológicas, Buenos Aires, ArgentinaLaboratorio de Resistencia Bacteriana, Cátedra de Microbiología, Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Buenos Aires, ArgentinaLaboratorio de Bacteriología General, Instituto de Patobiología, Centro Nacional de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Tecnológicas, Buenos Aires, ArgentinaThe use of antimicrobial growth promoters (AGPs) in sub-therapeutic doses for long periods promotes the selection of resistant microorganisms and the subsequent risk of spreading this resistance to the human population and the environment. Global concern about antimicrobial resistance development and transference of resistance genes from animal to human has been rising. The goal of our research was to evaluate the susceptibility pattern to different classes of antimicrobials of colistin-resistant Escherichia coli from poultry production systems that use AGPs, and characterize the resistance determinants associated to transferable platforms. E. coli strains (n = 41) were obtained from fecal samples collected from typical Argentine commercial broiler farms and susceptibility for 23 antimicrobials, relevant for human or veterinary medicine, was determined. Isolates were tested by PCR for the presence of mcr-1, extended spectrum β-lactamase encoding genes and plasmid-mediated quinolone resistance (PMQR) coding genes. Conjugation and susceptibility patterns of the transconjugant studies were performed. ERIC-PCR and REP-PCR analysis showed a high diversity of the isolates. Resistance to several antimicrobials was determined and all colistin-resistant isolates harbored the mcr-1 gene. CTX-M-2 cefotaximase was the main mechanism responsible for third generation cephalosporins resistance, and PMQR determinants were also identified. In addition, co-transference of the qnrB determinant on the mcr-1-positive transconjugants was corroborated, which suggests that these resistance genes are likely to be located in the same plasmid. In this work a wide range of antimicrobial resistance mechanisms were identified in E. coli strains isolated from the environment of healthy chickens highlighting the risk of antimicrobial abuse/misuse in animals under intensive production systems and its consequences for public health.https://www.frontiersin.org/article/10.3389/fmicb.2018.01679/fullColistinmcr-1food-borne bacteriaEscherichia coliCTX-M-2qnrB |
| spellingShingle | Johana E. Dominguez Johana E. Dominguez Leandro M. Redondo Leandro M. Redondo Roque A. Figueroa Espinosa Roque A. Figueroa Espinosa Daniela Cejas Daniela Cejas Gabriel O. Gutkind Gabriel O. Gutkind Pablo A. Chacana José A. Di Conza José A. Di Conza Mariano E. Fernández Miyakawa Mariano E. Fernández Miyakawa Simultaneous Carriage of mcr-1 and Other Antimicrobial Resistance Determinants in Escherichia coli From Poultry Frontiers in Microbiology Colistin mcr-1 food-borne bacteria Escherichia coli CTX-M-2 qnrB |
| title | Simultaneous Carriage of mcr-1 and Other Antimicrobial Resistance Determinants in Escherichia coli From Poultry |
| title_full | Simultaneous Carriage of mcr-1 and Other Antimicrobial Resistance Determinants in Escherichia coli From Poultry |
| title_fullStr | Simultaneous Carriage of mcr-1 and Other Antimicrobial Resistance Determinants in Escherichia coli From Poultry |
| title_full_unstemmed | Simultaneous Carriage of mcr-1 and Other Antimicrobial Resistance Determinants in Escherichia coli From Poultry |
| title_short | Simultaneous Carriage of mcr-1 and Other Antimicrobial Resistance Determinants in Escherichia coli From Poultry |
| title_sort | simultaneous carriage of mcr 1 and other antimicrobial resistance determinants in escherichia coli from poultry |
| topic | Colistin mcr-1 food-borne bacteria Escherichia coli CTX-M-2 qnrB |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.01679/full |
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