Responses of microRNA in digestive glands of mussel Mytilus galloprovincialis exposed to polystyrene nanoplastics
Polystyrene nanoplastics (PS-NPs) are typical accumulated nanoplastics in the marine environment and organisms, and have strong potential risks to marine ecological environment and human health. MiRNAs could respond to and participate in the response process of environmental stressors. However, the...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322012520 |
| _version_ | 1797961297077207040 |
|---|---|
| author | Zhen Lu Sihan Wu Zeyu Xiao Jun Song Huifeng Wu Xiao Peng |
| author_facet | Zhen Lu Sihan Wu Zeyu Xiao Jun Song Huifeng Wu Xiao Peng |
| author_sort | Zhen Lu |
| collection | DOAJ |
| description | Polystyrene nanoplastics (PS-NPs) are typical accumulated nanoplastics in the marine environment and organisms, and have strong potential risks to marine ecological environment and human health. MiRNAs could respond to and participate in the response process of environmental stressors. However, the response of miRNAs to nanoplastics has not been fully explored. In this study, miRNA responses of digestive glands in mussels Mytilus galloprovincialis treated by 200 nm PS-NPs (20, 200, 2000 μg/L) for 7 days were characterized by BGISEQ-500 deep sequencing and bioinformatics analysis, along with histopathological quantification with planimetric parameters on hematoxylin and eosin (H&E) staining. Results showed that one novel miRNA (novel_mir63) and seven known miRNAs (miR-34_2, miR-34_5, miR-281_8, let-7–5p_6, miR-10, miR-124, miR-29b-3p) were significantly (adjusted P-value < 0.05) differentially expressed after PS-NPs treatments, and most of them were down-regulated expect for novel_mir63 and miR-34_2. Function analysis of target genes corresponding to these differentially expressed miRNAs indicated that PS-NPs disturbed the process related to metabolism, aging, cardiac function, neural excitation, and repairment. Among them, acetyl-CoA C-acetyltransferase and purine metabolism pathway played vital connection roles. Meanwhile, significantly morphology changes of digestive tubes obtained from H&E stained sections also implied severely disrupted metabolic capability in digestive glands, reflected by significantly increased mean diverticular radius (MDR) and mean luminal radius (MLR) values and the ratio of MLR to mean epithelial thickness (MET), and significantly decreased MET value and MET/MDR. Overall, these findings have revealed new characterization of miRNAs and their target genes in mussel M. galloprovincialis under PS-NPs stress, and provide important clues to further elucidate the toxicity mechanisms of PS-NPs. |
| first_indexed | 2024-04-11T00:56:46Z |
| format | Article |
| id | doaj.art-a7dd5860651b455ca79da268f6f6b98d |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-11T00:56:46Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-a7dd5860651b455ca79da268f6f6b98d2023-01-05T04:30:35ZengElsevierEcotoxicology and Environmental Safety0147-65132023-01-01249114412Responses of microRNA in digestive glands of mussel Mytilus galloprovincialis exposed to polystyrene nanoplasticsZhen Lu0Sihan Wu1Zeyu Xiao2Jun Song3Huifeng Wu4Xiao Peng5CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), YICCAS, Yantai 264003, PR China; Center for Biomedical Optics and Photonics (CBOP) & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems, Shenzhen University, Shenzhen 518060, PR ChinaCenter for Biomedical Optics and Photonics (CBOP) & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems, Shenzhen University, Shenzhen 518060, PR ChinaCenter for Biomedical Optics and Photonics (CBOP) & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems, Shenzhen University, Shenzhen 518060, PR ChinaCenter for Biomedical Optics and Photonics (CBOP) & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems, Shenzhen University, Shenzhen 518060, PR ChinaCAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), YICCAS, Yantai 264003, PR China; Corresponding authors.Center for Biomedical Optics and Photonics (CBOP) & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems, Shenzhen University, Shenzhen 518060, PR China; Corresponding authors.Polystyrene nanoplastics (PS-NPs) are typical accumulated nanoplastics in the marine environment and organisms, and have strong potential risks to marine ecological environment and human health. MiRNAs could respond to and participate in the response process of environmental stressors. However, the response of miRNAs to nanoplastics has not been fully explored. In this study, miRNA responses of digestive glands in mussels Mytilus galloprovincialis treated by 200 nm PS-NPs (20, 200, 2000 μg/L) for 7 days were characterized by BGISEQ-500 deep sequencing and bioinformatics analysis, along with histopathological quantification with planimetric parameters on hematoxylin and eosin (H&E) staining. Results showed that one novel miRNA (novel_mir63) and seven known miRNAs (miR-34_2, miR-34_5, miR-281_8, let-7–5p_6, miR-10, miR-124, miR-29b-3p) were significantly (adjusted P-value < 0.05) differentially expressed after PS-NPs treatments, and most of them were down-regulated expect for novel_mir63 and miR-34_2. Function analysis of target genes corresponding to these differentially expressed miRNAs indicated that PS-NPs disturbed the process related to metabolism, aging, cardiac function, neural excitation, and repairment. Among them, acetyl-CoA C-acetyltransferase and purine metabolism pathway played vital connection roles. Meanwhile, significantly morphology changes of digestive tubes obtained from H&E stained sections also implied severely disrupted metabolic capability in digestive glands, reflected by significantly increased mean diverticular radius (MDR) and mean luminal radius (MLR) values and the ratio of MLR to mean epithelial thickness (MET), and significantly decreased MET value and MET/MDR. Overall, these findings have revealed new characterization of miRNAs and their target genes in mussel M. galloprovincialis under PS-NPs stress, and provide important clues to further elucidate the toxicity mechanisms of PS-NPs.http://www.sciencedirect.com/science/article/pii/S0147651322012520Polystyrene nanoplastics (PS-NPs)MusselMicroRNAs (miRNA)Toxicological effectsMetabolism |
| spellingShingle | Zhen Lu Sihan Wu Zeyu Xiao Jun Song Huifeng Wu Xiao Peng Responses of microRNA in digestive glands of mussel Mytilus galloprovincialis exposed to polystyrene nanoplastics Ecotoxicology and Environmental Safety Polystyrene nanoplastics (PS-NPs) Mussel MicroRNAs (miRNA) Toxicological effects Metabolism |
| title | Responses of microRNA in digestive glands of mussel Mytilus galloprovincialis exposed to polystyrene nanoplastics |
| title_full | Responses of microRNA in digestive glands of mussel Mytilus galloprovincialis exposed to polystyrene nanoplastics |
| title_fullStr | Responses of microRNA in digestive glands of mussel Mytilus galloprovincialis exposed to polystyrene nanoplastics |
| title_full_unstemmed | Responses of microRNA in digestive glands of mussel Mytilus galloprovincialis exposed to polystyrene nanoplastics |
| title_short | Responses of microRNA in digestive glands of mussel Mytilus galloprovincialis exposed to polystyrene nanoplastics |
| title_sort | responses of microrna in digestive glands of mussel mytilus galloprovincialis exposed to polystyrene nanoplastics |
| topic | Polystyrene nanoplastics (PS-NPs) Mussel MicroRNAs (miRNA) Toxicological effects Metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322012520 |
| work_keys_str_mv | AT zhenlu responsesofmicrornaindigestiveglandsofmusselmytilusgalloprovincialisexposedtopolystyrenenanoplastics AT sihanwu responsesofmicrornaindigestiveglandsofmusselmytilusgalloprovincialisexposedtopolystyrenenanoplastics AT zeyuxiao responsesofmicrornaindigestiveglandsofmusselmytilusgalloprovincialisexposedtopolystyrenenanoplastics AT junsong responsesofmicrornaindigestiveglandsofmusselmytilusgalloprovincialisexposedtopolystyrenenanoplastics AT huifengwu responsesofmicrornaindigestiveglandsofmusselmytilusgalloprovincialisexposedtopolystyrenenanoplastics AT xiaopeng responsesofmicrornaindigestiveglandsofmusselmytilusgalloprovincialisexposedtopolystyrenenanoplastics |