Wrinkling on Stimuli-Responsive Functional Polymer Surfaces as a Promising Strategy for the Preparation of Effective Antibacterial/Antibiofouling Surfaces
Biocompatible smart interfaces play a crucial role in biomedical or tissue engineering applications, where their ability to actively change their conformation or physico-chemical properties permits finely tuning their surface attributes. Polyelectrolytes, such as acrylic acid, are a particular type...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/23/4262 |
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| author | Carmen M. González-Henríquez Fernando E. Rodríguez-Umanzor Matías N. Alegría-Gómez Claudio A. Terraza-Inostroza Enrique Martínez-Campos Raquel Cue-López Mauricio A. Sarabia-Vallejos Claudio García-Herrera Juan Rodríguez-Hernández |
| author_facet | Carmen M. González-Henríquez Fernando E. Rodríguez-Umanzor Matías N. Alegría-Gómez Claudio A. Terraza-Inostroza Enrique Martínez-Campos Raquel Cue-López Mauricio A. Sarabia-Vallejos Claudio García-Herrera Juan Rodríguez-Hernández |
| author_sort | Carmen M. González-Henríquez |
| collection | DOAJ |
| description | Biocompatible smart interfaces play a crucial role in biomedical or tissue engineering applications, where their ability to actively change their conformation or physico-chemical properties permits finely tuning their surface attributes. Polyelectrolytes, such as acrylic acid, are a particular type of smart polymers that present pH responsiveness. This work aims to fabricate stable hydrogel films with reversible pH responsiveness that could spontaneously form wrinkled surface patterns. For this purpose, the photosensitive reaction mixtures were deposited via spin-coating over functionalized glasses. Following vacuum, UV, or either plasma treatments, it is possible to spontaneously form wrinkles, which could increase cell adherence. The pH responsiveness of the material was evaluated, observing an abrupt variation in the film thickness as a function of the environmental pH. Moreover, the presence of the carboxylic acid functional groups at the interface was evidenced by analyzing the adsorption/desorption capacity using methylene blue as a cationic dye model. The results demonstrated that increasing the acrylic acid in the microwrinkled hydrogel effectively improved the adsorption and release capacity and the ability of the carboxylic groups to establish ionic interactions with methylene blue. Finally, the role of the acrylic acid groups and the surface topography (smooth or wrinkled) on the final antibacterial properties were investigated, demonstrating their efficacy against both gram-positive and gram-negative bacteria model strains (<i>E. coli</i> and <i>S. Aureus</i>). According to our findings, microwrinkled hydrogels presented excellent antibacterial properties improving the results obtained for planar (smooth) hydrogels. |
| first_indexed | 2024-03-10T04:46:40Z |
| format | Article |
| id | doaj.art-a7e18c98d6d142a493e5094c842b73d1 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T04:46:40Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-a7e18c98d6d142a493e5094c842b73d12023-11-23T02:55:21ZengMDPI AGPolymers2073-43602021-12-011323426210.3390/polym13234262Wrinkling on Stimuli-Responsive Functional Polymer Surfaces as a Promising Strategy for the Preparation of Effective Antibacterial/Antibiofouling SurfacesCarmen M. González-Henríquez0Fernando E. Rodríguez-Umanzor1Matías N. Alegría-Gómez2Claudio A. Terraza-Inostroza3Enrique Martínez-Campos4Raquel Cue-López5Mauricio A. Sarabia-Vallejos6Claudio García-Herrera7Juan Rodríguez-Hernández8Departamento de Química, Facultad de Ciencias Naturales, Matemáticas y del Medio Ambiente, Universidad Tecnológica Metropolitana, Santiago 7800003, ChileDepartamento de Química, Facultad de Ciencias Naturales, Matemáticas y del Medio Ambiente, Universidad Tecnológica Metropolitana, Santiago 7800003, ChileDepartamento de Química, Facultad de Ciencias Naturales, Matemáticas y del Medio Ambiente, Universidad Tecnológica Metropolitana, Santiago 7800003, ChileResearch Laboratory for Organic Polymer (RLOP), Facultad de Química y Farmacia, Pontificia Universidad Católica de Chile, Santiago 7810000, ChileGroup of Organic Synthesis and Bioevaluation, Instituto Pluridisciplinar, Universidad Complutense de Madrid, Associated Unit to the ICTP-IQM-CSIC, 28040 Madrid, SpainGroup of Organic Synthesis and Bioevaluation, Instituto Pluridisciplinar, Universidad Complutense de Madrid, Associated Unit to the ICTP-IQM-CSIC, 28040 Madrid, SpainDepartamento de Ingeniería Mecánica, Facultad de Ingeniería, Universidad de Santiago de Chile, Santiago 9170022, ChileDepartamento de Ingeniería Mecánica, Facultad de Ingeniería, Universidad de Santiago de Chile, Santiago 9170022, ChilePolymer Functionalization Group, Departamento de Química Macromolecular Aplicada, Instituto de Ciencia y Tecnología de Polímeros-Consejo Superior de Investigaciones Científicas (ICTP-CSIC), 28006 Madrid, SpainBiocompatible smart interfaces play a crucial role in biomedical or tissue engineering applications, where their ability to actively change their conformation or physico-chemical properties permits finely tuning their surface attributes. Polyelectrolytes, such as acrylic acid, are a particular type of smart polymers that present pH responsiveness. This work aims to fabricate stable hydrogel films with reversible pH responsiveness that could spontaneously form wrinkled surface patterns. For this purpose, the photosensitive reaction mixtures were deposited via spin-coating over functionalized glasses. Following vacuum, UV, or either plasma treatments, it is possible to spontaneously form wrinkles, which could increase cell adherence. The pH responsiveness of the material was evaluated, observing an abrupt variation in the film thickness as a function of the environmental pH. Moreover, the presence of the carboxylic acid functional groups at the interface was evidenced by analyzing the adsorption/desorption capacity using methylene blue as a cationic dye model. The results demonstrated that increasing the acrylic acid in the microwrinkled hydrogel effectively improved the adsorption and release capacity and the ability of the carboxylic groups to establish ionic interactions with methylene blue. Finally, the role of the acrylic acid groups and the surface topography (smooth or wrinkled) on the final antibacterial properties were investigated, demonstrating their efficacy against both gram-positive and gram-negative bacteria model strains (<i>E. coli</i> and <i>S. Aureus</i>). According to our findings, microwrinkled hydrogels presented excellent antibacterial properties improving the results obtained for planar (smooth) hydrogels.https://www.mdpi.com/2073-4360/13/23/4262pH-sensitive hydrogelpoly(acrylic acid)quartz crystal microbalance (QCM)ellipsometric measurementantibacterial activity |
| spellingShingle | Carmen M. González-Henríquez Fernando E. Rodríguez-Umanzor Matías N. Alegría-Gómez Claudio A. Terraza-Inostroza Enrique Martínez-Campos Raquel Cue-López Mauricio A. Sarabia-Vallejos Claudio García-Herrera Juan Rodríguez-Hernández Wrinkling on Stimuli-Responsive Functional Polymer Surfaces as a Promising Strategy for the Preparation of Effective Antibacterial/Antibiofouling Surfaces Polymers pH-sensitive hydrogel poly(acrylic acid) quartz crystal microbalance (QCM) ellipsometric measurement antibacterial activity |
| title | Wrinkling on Stimuli-Responsive Functional Polymer Surfaces as a Promising Strategy for the Preparation of Effective Antibacterial/Antibiofouling Surfaces |
| title_full | Wrinkling on Stimuli-Responsive Functional Polymer Surfaces as a Promising Strategy for the Preparation of Effective Antibacterial/Antibiofouling Surfaces |
| title_fullStr | Wrinkling on Stimuli-Responsive Functional Polymer Surfaces as a Promising Strategy for the Preparation of Effective Antibacterial/Antibiofouling Surfaces |
| title_full_unstemmed | Wrinkling on Stimuli-Responsive Functional Polymer Surfaces as a Promising Strategy for the Preparation of Effective Antibacterial/Antibiofouling Surfaces |
| title_short | Wrinkling on Stimuli-Responsive Functional Polymer Surfaces as a Promising Strategy for the Preparation of Effective Antibacterial/Antibiofouling Surfaces |
| title_sort | wrinkling on stimuli responsive functional polymer surfaces as a promising strategy for the preparation of effective antibacterial antibiofouling surfaces |
| topic | pH-sensitive hydrogel poly(acrylic acid) quartz crystal microbalance (QCM) ellipsometric measurement antibacterial activity |
| url | https://www.mdpi.com/2073-4360/13/23/4262 |
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