PRMT4 is a novel coactivator of c-Myb-dependent transcription in haematopoietic cell lines.
Protein arginine methyltransferase 4 (PRMT4)-dependent methylation of arginine residues in histones and other chromatin-associated proteins plays an important role in the regulation of gene expression. However, the exact mechanism of how PRMT4 activates transcription remains elusive. Here, we identi...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC3591284?pdf=render |
| _version_ | 1819016620938362880 |
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| author | Gundula Streubel Caroline Bouchard Hannah Berberich Marc S Zeller Sophia Teichmann Jürgen Adamkiewicz Rolf Müller Karl-Heinz Klempnauer Uta-Maria Bauer |
| author_facet | Gundula Streubel Caroline Bouchard Hannah Berberich Marc S Zeller Sophia Teichmann Jürgen Adamkiewicz Rolf Müller Karl-Heinz Klempnauer Uta-Maria Bauer |
| author_sort | Gundula Streubel |
| collection | DOAJ |
| description | Protein arginine methyltransferase 4 (PRMT4)-dependent methylation of arginine residues in histones and other chromatin-associated proteins plays an important role in the regulation of gene expression. However, the exact mechanism of how PRMT4 activates transcription remains elusive. Here, we identify the chromatin remodeller Mi2α as a novel interaction partner of PRMT4. PRMT4 binds Mi2α and its close relative Mi2β, but not the other components of the repressive Mi2-containing NuRD complex. In the search for the biological role of this interaction, we find that PRMT4 and Mi2α/β interact with the transcription factor c-Myb and cooperatively coactivate c-Myb target gene expression in haematopoietic cell lines. This coactivation requires the methyltransferase and ATPase activity of PRMT4 and Mi2, respectively. Chromatin immunoprecipitation analysis shows that c-Myb target genes are direct transcriptional targets of PRMT4 and Mi2. Knockdown of PRMT4 or Mi2α/β in haematopoietic cells of the erythroid lineage results in diminished transcriptional induction of c-Myb target genes, attenuated cell growth and survival, and deregulated differentiation resembling the effects caused by c-Myb depletion. These findings reveal an important and so far unknown connection between PRMT4 and the chromatin remodeller Mi2 in c-Myb signalling. |
| first_indexed | 2024-12-21T02:50:32Z |
| format | Article |
| id | doaj.art-a7e528087cad46298917476ed0bdf21d |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-21T02:50:32Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-a7e528087cad46298917476ed0bdf21d2022-12-21T19:18:27ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042013-01-0193e100334310.1371/journal.pgen.1003343PRMT4 is a novel coactivator of c-Myb-dependent transcription in haematopoietic cell lines.Gundula StreubelCaroline BouchardHannah BerberichMarc S ZellerSophia TeichmannJürgen AdamkiewiczRolf MüllerKarl-Heinz KlempnauerUta-Maria BauerProtein arginine methyltransferase 4 (PRMT4)-dependent methylation of arginine residues in histones and other chromatin-associated proteins plays an important role in the regulation of gene expression. However, the exact mechanism of how PRMT4 activates transcription remains elusive. Here, we identify the chromatin remodeller Mi2α as a novel interaction partner of PRMT4. PRMT4 binds Mi2α and its close relative Mi2β, but not the other components of the repressive Mi2-containing NuRD complex. In the search for the biological role of this interaction, we find that PRMT4 and Mi2α/β interact with the transcription factor c-Myb and cooperatively coactivate c-Myb target gene expression in haematopoietic cell lines. This coactivation requires the methyltransferase and ATPase activity of PRMT4 and Mi2, respectively. Chromatin immunoprecipitation analysis shows that c-Myb target genes are direct transcriptional targets of PRMT4 and Mi2. Knockdown of PRMT4 or Mi2α/β in haematopoietic cells of the erythroid lineage results in diminished transcriptional induction of c-Myb target genes, attenuated cell growth and survival, and deregulated differentiation resembling the effects caused by c-Myb depletion. These findings reveal an important and so far unknown connection between PRMT4 and the chromatin remodeller Mi2 in c-Myb signalling.http://europepmc.org/articles/PMC3591284?pdf=render |
| spellingShingle | Gundula Streubel Caroline Bouchard Hannah Berberich Marc S Zeller Sophia Teichmann Jürgen Adamkiewicz Rolf Müller Karl-Heinz Klempnauer Uta-Maria Bauer PRMT4 is a novel coactivator of c-Myb-dependent transcription in haematopoietic cell lines. PLoS Genetics |
| title | PRMT4 is a novel coactivator of c-Myb-dependent transcription in haematopoietic cell lines. |
| title_full | PRMT4 is a novel coactivator of c-Myb-dependent transcription in haematopoietic cell lines. |
| title_fullStr | PRMT4 is a novel coactivator of c-Myb-dependent transcription in haematopoietic cell lines. |
| title_full_unstemmed | PRMT4 is a novel coactivator of c-Myb-dependent transcription in haematopoietic cell lines. |
| title_short | PRMT4 is a novel coactivator of c-Myb-dependent transcription in haematopoietic cell lines. |
| title_sort | prmt4 is a novel coactivator of c myb dependent transcription in haematopoietic cell lines |
| url | http://europepmc.org/articles/PMC3591284?pdf=render |
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