Comparative Study on Pharmacokinetics of Four Long-Acting Injectable Formulations of Enrofloxacin in Pigs
A comparative study on pharmacokinetics of four long-acting enrofloxacin injectable formulations was investigated in 36 healthy pigs after intramuscular injection according to the recommended single dose @ 2.5 mg/kg body weight. The drug concentrations in the plasma were computed using high-performa...
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2020.604628/full |
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| author | Salah Uddin Ahmad Salah Uddin Ahmad Salah Uddin Ahmad Salah Uddin Ahmad Jichao Sun Jichao Sun Jichao Sun Fusheng Cheng Fusheng Cheng Fusheng Cheng Bing Li Bing Li Bing Li Safia Arbab Safia Arbab Safia Arbab Xuzheng Zhou Xuzheng Zhou Xuzheng Zhou Jiyu Zhang Jiyu Zhang Jiyu Zhang |
| author_facet | Salah Uddin Ahmad Salah Uddin Ahmad Salah Uddin Ahmad Salah Uddin Ahmad Jichao Sun Jichao Sun Jichao Sun Fusheng Cheng Fusheng Cheng Fusheng Cheng Bing Li Bing Li Bing Li Safia Arbab Safia Arbab Safia Arbab Xuzheng Zhou Xuzheng Zhou Xuzheng Zhou Jiyu Zhang Jiyu Zhang Jiyu Zhang |
| author_sort | Salah Uddin Ahmad |
| collection | DOAJ |
| description | A comparative study on pharmacokinetics of four long-acting enrofloxacin injectable formulations was investigated in 36 healthy pigs after intramuscular injection according to the recommended single dose @ 2.5 mg/kg body weight. The drug concentrations in the plasma were computed using high-performance liquid chromatography (HPLC) with fluorescence detection. WinNonLin5.2.1 software was used to analyze the experimental data and compared it under one-way ANOVA using SPSS software with a 95% confidence interval (CI). The main pharmacokinetic parameters, that is, the maximum plasma concentrations (Cmax), the time to maximum concentration (Tmax), area under the time curve concentration (AUCall) and Terminal half-life (T1/2) were 733.84 ± 129.87, 917.00 ± 240.13, 694.84 ± 163.49, 621.98 ± 227.25 ng/ml, 2.19 ± 0.0.66, 1.50 ± 0.37, 2.89 ± 0.24, 0.34 ± 0.13 h, 7754.43 ± 2887.16, 8084.11 ± 1543.98, 7369.42 ± 2334.99, 4194.10 ± 1186.62 ng h/ml, 10.48 ± 2.72, 10.37 ± 2.38, 10.20 ± 2.81, and 10.61 ± 0.86 h for 10% enrofloxacin (Alkali), 20% enrofloxacin (Acidic), Yangkang and control drug Nuokang® respectively. There were significant differences among Cmax, Tmax, and AUCall of three formulations compare with that of the reference formulation. No significant differences were observed among the T1/2 for tested formulations compare with the reference formulation. The pharmacokinetic parameters showed that the tested formulations were somewhat better compared to the reference one. The calculated PK/PD indices were effective for bacteria such as Actinobacillus pleuropneumoniae and Pasteurella multocida with values higher than the cut-off points (Cmax/MIC90≥10–12 and AUC/MIC90 ≥ 125). However, they were not effective against bacteria like Haemophilus parasuis, Streptococcus suis, E. coli, and Bordetella bronchiseptica where lower values were obtained. |
| first_indexed | 2024-12-20T14:47:02Z |
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| id | doaj.art-a7eb2d28a5624dc2a5759fa9ccfae4db |
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| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-20T14:47:02Z |
| publishDate | 2021-01-01 |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-a7eb2d28a5624dc2a5759fa9ccfae4db2022-12-21T19:37:06ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-01-01710.3389/fvets.2020.604628604628Comparative Study on Pharmacokinetics of Four Long-Acting Injectable Formulations of Enrofloxacin in PigsSalah Uddin Ahmad0Salah Uddin Ahmad1Salah Uddin Ahmad2Salah Uddin Ahmad3Jichao Sun4Jichao Sun5Jichao Sun6Fusheng Cheng7Fusheng Cheng8Fusheng Cheng9Bing Li10Bing Li11Bing Li12Safia Arbab13Safia Arbab14Safia Arbab15Xuzheng Zhou16Xuzheng Zhou17Xuzheng Zhou18Jiyu Zhang19Jiyu Zhang20Jiyu Zhang21Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Lanzhou, ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, ChinaFaculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, BangladeshKey Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Lanzhou, ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Lanzhou, ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Lanzhou, ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Lanzhou, ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Lanzhou, ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Lanzhou, ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, ChinaA comparative study on pharmacokinetics of four long-acting enrofloxacin injectable formulations was investigated in 36 healthy pigs after intramuscular injection according to the recommended single dose @ 2.5 mg/kg body weight. The drug concentrations in the plasma were computed using high-performance liquid chromatography (HPLC) with fluorescence detection. WinNonLin5.2.1 software was used to analyze the experimental data and compared it under one-way ANOVA using SPSS software with a 95% confidence interval (CI). The main pharmacokinetic parameters, that is, the maximum plasma concentrations (Cmax), the time to maximum concentration (Tmax), area under the time curve concentration (AUCall) and Terminal half-life (T1/2) were 733.84 ± 129.87, 917.00 ± 240.13, 694.84 ± 163.49, 621.98 ± 227.25 ng/ml, 2.19 ± 0.0.66, 1.50 ± 0.37, 2.89 ± 0.24, 0.34 ± 0.13 h, 7754.43 ± 2887.16, 8084.11 ± 1543.98, 7369.42 ± 2334.99, 4194.10 ± 1186.62 ng h/ml, 10.48 ± 2.72, 10.37 ± 2.38, 10.20 ± 2.81, and 10.61 ± 0.86 h for 10% enrofloxacin (Alkali), 20% enrofloxacin (Acidic), Yangkang and control drug Nuokang® respectively. There were significant differences among Cmax, Tmax, and AUCall of three formulations compare with that of the reference formulation. No significant differences were observed among the T1/2 for tested formulations compare with the reference formulation. The pharmacokinetic parameters showed that the tested formulations were somewhat better compared to the reference one. The calculated PK/PD indices were effective for bacteria such as Actinobacillus pleuropneumoniae and Pasteurella multocida with values higher than the cut-off points (Cmax/MIC90≥10–12 and AUC/MIC90 ≥ 125). However, they were not effective against bacteria like Haemophilus parasuis, Streptococcus suis, E. coli, and Bordetella bronchiseptica where lower values were obtained.https://www.frontiersin.org/articles/10.3389/fvets.2020.604628/fullpharmacokineticslong-actingenrofloxacinHPLCpig |
| spellingShingle | Salah Uddin Ahmad Salah Uddin Ahmad Salah Uddin Ahmad Salah Uddin Ahmad Jichao Sun Jichao Sun Jichao Sun Fusheng Cheng Fusheng Cheng Fusheng Cheng Bing Li Bing Li Bing Li Safia Arbab Safia Arbab Safia Arbab Xuzheng Zhou Xuzheng Zhou Xuzheng Zhou Jiyu Zhang Jiyu Zhang Jiyu Zhang Comparative Study on Pharmacokinetics of Four Long-Acting Injectable Formulations of Enrofloxacin in Pigs Frontiers in Veterinary Science pharmacokinetics long-acting enrofloxacin HPLC pig |
| title | Comparative Study on Pharmacokinetics of Four Long-Acting Injectable Formulations of Enrofloxacin in Pigs |
| title_full | Comparative Study on Pharmacokinetics of Four Long-Acting Injectable Formulations of Enrofloxacin in Pigs |
| title_fullStr | Comparative Study on Pharmacokinetics of Four Long-Acting Injectable Formulations of Enrofloxacin in Pigs |
| title_full_unstemmed | Comparative Study on Pharmacokinetics of Four Long-Acting Injectable Formulations of Enrofloxacin in Pigs |
| title_short | Comparative Study on Pharmacokinetics of Four Long-Acting Injectable Formulations of Enrofloxacin in Pigs |
| title_sort | comparative study on pharmacokinetics of four long acting injectable formulations of enrofloxacin in pigs |
| topic | pharmacokinetics long-acting enrofloxacin HPLC pig |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2020.604628/full |
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