Phylogeography and Phylogenetic Evolution in Tibetan Sheep Based on <i>MT-CYB</i> Sequences
To date, molecular genetics and population studies in Tibetan sheep (<i>Ovis aries</i>) have been limited, and little is known about the phylogenetic evolution and phylogeography of Tibetan sheep populations. The aim of the present research was to explore phylogeography and phylogenetic...
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MDPI AG
2020-07-01
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| Online Access: | https://www.mdpi.com/2076-2615/10/7/1177 |
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| author | Jianbin Liu Zengkui Lu Chao Yuan Fan Wang Bohui Yang |
| author_facet | Jianbin Liu Zengkui Lu Chao Yuan Fan Wang Bohui Yang |
| author_sort | Jianbin Liu |
| collection | DOAJ |
| description | To date, molecular genetics and population studies in Tibetan sheep (<i>Ovis aries</i>) have been limited, and little is known about the phylogenetic evolution and phylogeography of Tibetan sheep populations. The aim of the present research was to explore phylogeography and phylogenetic evolution of Tibetan sheep populations, on the basis of mitochondrial DNA (mtDNA) gene <i>MT-CYB</i> (1140 base pairs). Our dataset consisted of 641 <i>MT-CYB</i> sequences from the same amount of animals belonging to 15 populations of Tibetan sheep living in the Qinghai–Tibetan Plateau, China. Haplotype and nucleotide diversities were 0.748 ± 0.010 and 0.003 ± 0.001, respectively. The analysis of phylogeography revealed the presence of two formerly described haplogroups in 15 populations of Tibetan sheep, however only one haplogroup was present in Awang sheep. Moreover, 641 Tibetan sheep were distributed into a minimum of two clusters by clustering analysis. The 15 Tibetan sheep populations and 19 reference populations of 878 individuals were separated into six main groups based on their substitutions per site, from which we constructed a phylogenetic tree. Minor differences in branching order of various taxa between trees acquired from either gene were observed. This study provides insights on the origins and phylogenetic evolution of populations residing in the Qinghai–Tibetan Plateau, which will aid information of future conservation programs aimed at conserving this valuable genetic resource. |
| first_indexed | 2024-03-10T18:31:49Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T18:31:49Z |
| publishDate | 2020-07-01 |
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| spelling | doaj.art-a7fa767f491149b58ea6e949eedb4edd2023-11-20T06:33:45ZengMDPI AGAnimals2076-26152020-07-01107117710.3390/ani10071177Phylogeography and Phylogenetic Evolution in Tibetan Sheep Based on <i>MT-CYB</i> SequencesJianbin Liu0Zengkui Lu1Chao Yuan2Fan Wang3Bohui Yang4Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaChina Agricultural Veterinarian Biology Science and Technology Co. Ltd., Lanzhou 730046, ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaTo date, molecular genetics and population studies in Tibetan sheep (<i>Ovis aries</i>) have been limited, and little is known about the phylogenetic evolution and phylogeography of Tibetan sheep populations. The aim of the present research was to explore phylogeography and phylogenetic evolution of Tibetan sheep populations, on the basis of mitochondrial DNA (mtDNA) gene <i>MT-CYB</i> (1140 base pairs). Our dataset consisted of 641 <i>MT-CYB</i> sequences from the same amount of animals belonging to 15 populations of Tibetan sheep living in the Qinghai–Tibetan Plateau, China. Haplotype and nucleotide diversities were 0.748 ± 0.010 and 0.003 ± 0.001, respectively. The analysis of phylogeography revealed the presence of two formerly described haplogroups in 15 populations of Tibetan sheep, however only one haplogroup was present in Awang sheep. Moreover, 641 Tibetan sheep were distributed into a minimum of two clusters by clustering analysis. The 15 Tibetan sheep populations and 19 reference populations of 878 individuals were separated into six main groups based on their substitutions per site, from which we constructed a phylogenetic tree. Minor differences in branching order of various taxa between trees acquired from either gene were observed. This study provides insights on the origins and phylogenetic evolution of populations residing in the Qinghai–Tibetan Plateau, which will aid information of future conservation programs aimed at conserving this valuable genetic resource.https://www.mdpi.com/2076-2615/10/7/1177Tibetan sheep<i>MT-CYB</i>phylogeographymaternal lineage |
| spellingShingle | Jianbin Liu Zengkui Lu Chao Yuan Fan Wang Bohui Yang Phylogeography and Phylogenetic Evolution in Tibetan Sheep Based on <i>MT-CYB</i> Sequences Animals Tibetan sheep <i>MT-CYB</i> phylogeography maternal lineage |
| title | Phylogeography and Phylogenetic Evolution in Tibetan Sheep Based on <i>MT-CYB</i> Sequences |
| title_full | Phylogeography and Phylogenetic Evolution in Tibetan Sheep Based on <i>MT-CYB</i> Sequences |
| title_fullStr | Phylogeography and Phylogenetic Evolution in Tibetan Sheep Based on <i>MT-CYB</i> Sequences |
| title_full_unstemmed | Phylogeography and Phylogenetic Evolution in Tibetan Sheep Based on <i>MT-CYB</i> Sequences |
| title_short | Phylogeography and Phylogenetic Evolution in Tibetan Sheep Based on <i>MT-CYB</i> Sequences |
| title_sort | phylogeography and phylogenetic evolution in tibetan sheep based on i mt cyb i sequences |
| topic | Tibetan sheep <i>MT-CYB</i> phylogeography maternal lineage |
| url | https://www.mdpi.com/2076-2615/10/7/1177 |
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